Team:Hong Kong-CUHK/project-5.html

From 2014.igem.org

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<h2>Further direction</h2>
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        <h2>Future Application</h2>
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<p>The project is done little as the gene sequence of hydrogenase and nitrogenase cluster are of very long sequence (about 30kb in total). Following the failure with Gibson assembly for many times, the cluster is difficult to be made. Up to the time of wiki deadline, the hydrogenase and nitrogenase cluster is not finished. Lengthening the overlapping base pairs of Gibson assembly and other techniques such as overlapping pcr or synthesis may be an alternative way for finishing the project.</p>
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<a href="https://2014.igem.org/Team:Hong_Kong-CUHK/home.html?page=link-project">Back to the main Project Page</a>
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                    <strong><u>Control enzymatic reactions</u>
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                    <li>Voltage switch allows fast and easy control of enzymatic reaction rate by electricity.</li>
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                    <li>We are working on demonstrating this with laccase and dioxygenase, and also BiFC</li>
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                    <strong><u>Bacterial Screening</u>
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                    <li>Utilize the BiFC</li>
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                    <li>Load cells onto multi-well plates</li>
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                    <li>Each well serves as a Pixel</li>
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                    <img width="600" height="200" src="https://static.igem.org/mediawiki/2013/9/96/Sry12.png" alt="1">
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                    <strong><u>Degradation of cellular proteins</u>
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                    <li>Utilize the Ubiquitin/26S proteasome system</li>
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                    <li>Linking the voltage switch with E1/E2/E3 protein</li>
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                    <li>Control degradation rate of cellular proteins by controlling ubiquitinylation rate</li>
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                    <strong><u>Study genes using protein complementation</u>
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                    <li>Split genes into two parts, and insert into our system</li>
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                    <li>Use voltage switch to control the split and refold of a gene.</li>
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                    <li>Possible tool for the screening of new protein complementation compatible proteins.</li>
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                    <strong><u>Study Membrane Potential of Organelles</u>
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                    <li>Target voltage switch system with BiFC onto the membrane of organelles interested</li>
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                    <li>Obtain fluorescent signal</li>
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                    <li>Estimate membrane potential</li>
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Latest revision as of 04:16, 27 November 2014

Further direction

The project is done little as the gene sequence of hydrogenase and nitrogenase cluster are of very long sequence (about 30kb in total). Following the failure with Gibson assembly for many times, the cluster is difficult to be made. Up to the time of wiki deadline, the hydrogenase and nitrogenase cluster is not finished. Lengthening the overlapping base pairs of Gibson assembly and other techniques such as overlapping pcr or synthesis may be an alternative way for finishing the project.

<a href="https://2014.igem.org/Team:Hong_Kong-CUHK/home.html?page=link-project">Back to the main Project Page</a>

Retrieved from "http://2014.igem.org/Team:Hong_Kong-CUHK/project-5.html"