{"x/5QhtDbcBEqpCrjzQT":{"protocol":{"v":1,"name":"Make competent cells not yet carrying any plasmid","_id":"F2oNT7tGTyGFCjAB9"},"params":{"0ce97191c161424bd6b9642a75f54aca":{"product":"Culture","type":"experiment","text":"E. coli BL21(DE3)","experiment":"BpjoAZEF4hQB6qMnb","v":0}},"values":{},"finishDate":1406757600,"products":{"a0b4e4a2b22f540a0527e129d1f98684":{"type":{"name":"Chemically competent bacteria without any plasmid","v":0,"_id":"Xwooe4QfsLfskZNvG"},"text":"E. coli BL21(DE3)","allTypes":[{"_id":"nQbZRzR4ZeKkYMr8X","name":"Bacteria without any plasmid","v":0},{"v":0,"name":"Bacteria","_id":"YW7epnToTmqmHEEwx"},{"name":"Chemically competent bacteria without any plasmid","v":0,"_id":"Xwooe4QfsLfskZNvG"}],"properties":{"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)"}}}}},"x/nKLd5PXc2abg2hsGM":{"protocol":{"_id":"3cuNZHSvzbmc5wGLz","name":"Make a liquid culture without any plasmid","v":3},"params":{"87f8a6ab85c9ced3702b4ea641ad4bb5":{"v":1,"experiment":"aJkHPGccpLkiga4w5","type":"experiment","text":"Lysogeny broth + ","product":"Medium"},"4c0984e0a4d80995f3099e5c33188ce1":{"product":"Glycerol stock","experiment":"sP5kW8nnQ57CuJrY3","v":0,"text":"E. coli BL21(DE3)","type":"experiment"}},"finishDate":1406810220,"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"allTypes":[{"_id":"nQbZRzR4ZeKkYMr8X","v":0,"name":"Bacteria without any plasmid"},{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"8p9DTRXiSoymBbC3T","name":"Bacterial culture without any plasmid","v":1}],"type":{"name":"Bacterial culture without any plasmid","v":1,"_id":"8p9DTRXiSoymBbC3T"},"text":"E. coli BL21(DE3)","properties":{"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"BL21(DE3)"}}}},"values":{"1":{"0":"37","1":14.0666666666667},"0":{"0":"1","1":""}}},"x/aZsH9DxW73RSa5PrW":{"products":{"a0b4e4a2b22f540a0527e129d1f98684":{"properties":{"YW7epnToTmqmHEEwx":{"1ed8ac857097c4fbbbc15cd373ef96bc":"Turbo","e1520b5997a532c7889f6e8883920ab8":"E. coli"}},"allTypes":[{"_id":"nQbZRzR4ZeKkYMr8X","name":"Bacteria without any plasmid","v":0},{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"name":"Chemically competent bacteria without any plasmid","v":0,"_id":"Xwooe4QfsLfskZNvG"}],"type":{"_id":"Xwooe4QfsLfskZNvG","v":0,"name":"Chemically competent bacteria without any plasmid"},"text":"E. coli Turbo"}},"finishDate":1406930400,"values":{},"protocol":{"_id":"F2oNT7tGTyGFCjAB9","name":"Make competent cells not yet carrying any plasmid","v":1},"params":{"0ce97191c161424bd6b9642a75f54aca":{"product":"Culture","text":"E. coli Turbo","type":"experiment","experiment":"sxEwa4wmYAs7KsbQa","v":0}}},"x/A7aAaEvuWbNoFJkPT":{"params":{"5d37875fd1572e9ebc236d502ab9a20d":[{"values":{"10":{"0":"3.0"}},"paramValue":{"text":"DNA Ladder 1 kb (NEB)","type":"supply","supply":"HmEHKAkHuCtqDh9gd","v":0}},{"values":{"10":{"0":"20.8"}},"paramValue":{"product":"DNA","type":"experiment","text":"pSBX1T3-BBa_I13507 (E/S)","experiment":"uFeJY26RDky8eikPb","v":0}},{"values":{"10":{"0":"20.8"}},"paramValue":{"product":"DNA","type":"experiment","text":"pSBX1T3-BBa_I13507 (E/S)","v":0,"experiment":"uFeJY26RDky8eikPb"}},{"values":{"10":{"0":"20.8"}},"paramValue":{"type":"experiment","text":"pSBX4C5-BBa_I13507 (E/S)","v":0,"experiment":"Fgd6FSmqeLFwJLpzJ","product":"DNA"}},{"values":{"10":{"0":"20.8"}},"paramValue":{"product":"DNA","experiment":"Fgd6FSmqeLFwJLpzJ","v":0,"text":"pSBX4C5-BBa_I13507 (E/S)","type":"experiment"}},{"values":{"10":{"0":"20.8"}},"paramValue":{"type":"supply","text":"DNA Ladder 1 kb (NEB)","v":0,"supply":"HmEHKAkHuCtqDh9gd"}}]},"protocol":{"v":1,"name":"Gel Electrophoresis","_id":"JPmjNykwWASQiD9PX"},"values":{"11":{"1":"50","0":""}},"sid":"50","finishDate":1407003720,"products":{"12327177bfe077ccd10daa3d5ca9809c":{"allTypes":[{"v":0,"name":"Agarose Gel","_id":"QCyBocmukGKrzbkYb"}],"type":{"_id":"QCyBocmukGKrzbkYb","v":0,"name":"Agarose Gel"},"text":"","properties":{}}}},"x/RshcfopcqDkgw7Coj":{"products":{"9e2316f931255166f5f9a14d1e18a620":{"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"pSBX4C5-BBa_I13507 (E/S)","6f8ac39f837ee4598b7358ce18aec601":"R112","4da843835f15245d46602c3d57b579b6":"11"}},"allTypes":[{"v":3,"name":"dsDNA","_id":"k9LEWTcbmQn53Gzo7"}],"text":"pSBX4C5-BBa_I13507 (E/S)","type":{"_id":"k9LEWTcbmQn53Gzo7","name":"dsDNA","v":3}}},"finishDate":1407013200,"values":{"1":{"0":"R112"},"2":{"0":"11"},"0":{"1":"pSBX4C5-BBa_I13507 (E/S)","0":"50-4-1, 50-5-1"}},"protocol":{"_id":"hmhE7AHgjXE8Khw6M","name":"Gel extraction","v":2},"params":{"b7e16d839da3d1122a97f9d091ce6f91":[{"paramValue":{"product":"Gel","text":"","type":"experiment","v":0,"experiment":"A7aAaEvuWbNoFJkPT"},"values":{}}]}},"x/DQuqJeoBMhMGGadJP":{"params":{"64ce87c57c5b9a1a536ccd08a47740b3":{"product":"Agar plate","text":"E. coli TOP10 with pSBX4C5-T7RBS-IGEMHD_v3","type":"experiment","v":0,"experiment":"QyZDssCdmLc3NbXxE"}},"protocol":{"_id":"maDx7F6ry8Mw5JmKf","v":0,"name":"Pick a colony carrying a plasmid from an agar plate"},"values":{},"products":{"3bbfb159bcd6317e17bc0125824b5a25":{"type":{"name":"Bacteria carrying a plasmid","v":2,"_id":"PpBkfrLAqTw2CwNZX"},"text":"E. coli TOP10 with pSBX4C5-T7RBS-IGEMHD_v3","allTypes":[{"_id":"YW7epnToTmqmHEEwx","name":"Bacteria","v":0},{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2}],"properties":{"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"TOP10"},"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"C","0ac71517d2d368cdbabf8b9914716902":"pSBX4C5-T7RBS-IGEMHD_v3"}}}},"finishDate":1407013200},"x/wYJQifthWrcLajhaT":{"params":{"5d37875fd1572e9ebc236d502ab9a20d":[{"paramValue":{"product":"Excised DNA fragment","experiment":"RshcfopcqDkgw7Coj","v":0,"type":"experiment","text":"pSBX4C5-BBa_I13507 (E/S)"},"values":{"1":{"0":"6.468"}}},{"values":{"1":{"0":"0.282"}},"paramValue":{"text":"T7RBS-IGEMHD_v3","type":"experiment","experiment":"sLge6jeo2xtv3ptz5","v":0,"product":"dsDNA"}}]},"protocol":{"_id":"n4Sg8s8AfhMEtQtFH","v":1,"name":"Plasmid ligation with T4 DNA Ligase (NEB)"},"finishDate":1407013200,"products":{"63aa3271e10f79baaba8465140e427e9":{"text":"pSBX4C5-T7RBS-IGEMHD_v3 (S73#3)","type":{"_id":"cvt43tybpDTWdk2xR","name":"Plasmid","v":4},"allTypes":[{"_id":"k9LEWTcbmQn53Gzo7","v":3,"name":"dsDNA"},{"name":"Plasmid","v":4,"_id":"cvt43tybpDTWdk2xR"}],"properties":{"k9LEWTcbmQn53Gzo7":{"6f8ac39f837ee4598b7358ce18aec601":"S73#3","4da843835f15245d46602c3d57b579b6":"5.192753038","49ee3087348e8d44e1feda1917443987":"pSBX4C5-T7RBS-IGEMHD_v3"},"cvt43tybpDTWdk2xR":{"c29e15397d29fc94dad70016db7ab2e9":"C"}}}},"values":{"1":{"2":"S73#3","3":"C","1":"pSBX4C5-T7RBS-IGEMHD_v3","4":"5.192753038"},"0":{"0":"0","1":"7.5"}}},"x/TSRsASYGTtPMWxfhz":{"finishDate":1407016800,"products":{"79bb23c1a3d1c2c9104acc24452a9636":{"allTypes":[{"name":"dsDNA","v":3,"_id":"k9LEWTcbmQn53Gzo7"},{"v":4,"name":"Plasmid","_id":"cvt43tybpDTWdk2xR"}],"type":{"v":4,"name":"Plasmid","_id":"cvt43tybpDTWdk2xR"},"text":"pSBX4A5-T7RBS-LambdaLys-IGEMHD_v3 (A85)","properties":{"cvt43tybpDTWdk2xR":{"c29e15397d29fc94dad70016db7ab2e9":"A"},"k9LEWTcbmQn53Gzo7":{"6f8ac39f837ee4598b7358ce18aec601":"A85","4da843835f15245d46602c3d57b579b6":null,"49ee3087348e8d44e1feda1917443987":"pSBX4A5-T7RBS-LambdaLys-IGEMHD_v3"}}}},"values":{"2":{"5":null,"1":"64","3":"A","2":"pSBX4A5-T7RBS-LambdaLys-IGEMHD_v3","4":"A85","0":"72"},"1":{"0":"10"},"0":{"0":"1","3":"0","1":"9.53"}},"params":{"653ac32c6b9de96c55a6fcac6480ec00":{"product":"Excised DNA fragment","experiment":"2BRvSFvEmuqdDoD35","v":0,"type":"experiment","text":"pSBX4A5_taataa_atg"},"a458be0f08b7e4ff3c0f633c100176c0":[{"values":{"0":{"2":"0.47"}},"paramValue":{"v":0,"experiment":"kDEdxoHTJYMo8aeYp","text":"Lambda-Lys_pSBX_pSBX","type":"experiment","product":"Excised DNA fragment"}}]},"protocol":{"v":1,"name":"CPEC","_id":"z46dHCjoZpLkNgm4k"}},"x/XnL8PrNDhcjzrMLz8":{"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"PpBkfrLAqTw2CwNZX":{"0ac71517d2d368cdbabf8b9914716902":"pSBX4C5-T7RBS-IGEMHD_v3","10990dcf66373fff11ef907bc2b1ef97":"C"},"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"TOP10"}},"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","v":2,"name":"Bacteria carrying a plasmid"},{"_id":"YW7epnToTmqmHEEwx","name":"Bacteria","v":0},{"v":3,"name":"Bacterial culture carrying a plasmid","_id":"EZz9pqmLYbZXhdQuZ"}],"type":{"v":3,"name":"Bacterial culture carrying a plasmid","_id":"EZz9pqmLYbZXhdQuZ"},"text":"E. coli TOP10 with pSBX4C5-T7RBS-IGEMHD_v3"}},"finishDate":1407016800,"values":{"0":{"0":"","1":"C"},"1":{"1":0,"0":"37"}},"protocol":{"_id":"9TiKPrpdykEiSHFcy","v":5,"name":"Make a liquid culture carrying a plasmid"},"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"product":"Colony","type":"experiment","text":"E. coli TOP10 with pSBX4C5-T7RBS-IGEMHD_v3","v":0,"experiment":"DQuqJeoBMhMGGadJP"},"87f8a6ab85c9ced3702b4ea641ad4bb5":{"type":"experiment","text":"Lysogeny broth + ","v":1,"experiment":"aJkHPGccpLkiga4w5","product":"Medium"}}}}