{"x/ax9Bu2XGGX7uMK2Ss":{"protocol":{"name":"Make a liquid culture carrying a plasmid","v":5,"_id":"9TiKPrpdykEiSHFcy"},"params":{"4c0984e0a4d80995f3099e5c33188ce1":{"experiment":"qK76aj9dfYXZyZ6Rh","v":0,"type":"experiment","text":"E. coli OneShot with pUC19","product":"Culture"},"87f8a6ab85c9ced3702b4ea641ad4bb5":{"type":"experiment","text":"Lysogeny broth + ","v":1,"experiment":"aJkHPGccpLkiga4w5","product":"Medium"}},"finishDate":1403859600,"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"A","0ac71517d2d368cdbabf8b9914716902":"pUC19"},"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"OneShot"}},"type":{"name":"Bacterial culture carrying a plasmid","v":3,"_id":"EZz9pqmLYbZXhdQuZ"},"text":"E. coli OneShot with pUC19","allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","name":"Bacteria carrying a plasmid","v":2},{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"EZz9pqmLYbZXhdQuZ","v":3,"name":"Bacterial culture carrying a plasmid"}]}},"values":{"0":{"0":"15","1":"A"},"1":{"0":"37","1":2}}},"x/KDXh8d3RnXgWTHMQh":{"params":{"87f8a6ab85c9ced3702b4ea641ad4bb5":{"experiment":"aJkHPGccpLkiga4w5","v":1,"type":"experiment","text":"Lysogeny broth + ","product":"Medium"},"4c0984e0a4d80995f3099e5c33188ce1":{"text":"E. coli OneShot with pUC19","type":"experiment","v":0,"experiment":"ax9Bu2XGGX7uMK2Ss","product":"Culture"}},"protocol":{"name":"Make a liquid culture carrying a plasmid","v":5,"_id":"9TiKPrpdykEiSHFcy"},"finishDate":1403859900,"products":{"6926f2fd88f899a22a561f0e808aa8a9":{"properties":{"YW7epnToTmqmHEEwx":{"e1520b5997a532c7889f6e8883920ab8":"E. coli","1ed8ac857097c4fbbbc15cd373ef96bc":"OneShot"},"PpBkfrLAqTw2CwNZX":{"10990dcf66373fff11ef907bc2b1ef97":"A","0ac71517d2d368cdbabf8b9914716902":"pUC19"}},"text":"E. coli OneShot with pUC19","type":{"v":3,"name":"Bacterial culture carrying a plasmid","_id":"EZz9pqmLYbZXhdQuZ"},"allTypes":[{"_id":"PpBkfrLAqTw2CwNZX","v":2,"name":"Bacteria carrying a plasmid"},{"_id":"YW7epnToTmqmHEEwx","v":0,"name":"Bacteria"},{"_id":"EZz9pqmLYbZXhdQuZ","name":"Bacterial culture carrying a plasmid","v":3}]}},"values":{"0":{"1":"A","0":"1"},"1":{"0":"37","1":0.0833333333333333}}},"x/ES24yayg4haA44ZeD":{"values":{"0":{"1":"A","0":"50"}},"products":{"fccc3bbdd62f7ea544bf80e802c28b89":{"type":{"v":0,"name":"Agar plate with bacteria carrying a plasmid","_id":"D2DD2v2FYpYQLFwji"},"text":"E. coli OneShot with pUC19","allTypes":[{"_id":"D2DD2v2FYpYQLFwji","v":0,"name":"Agar plate with bacteria carrying a plasmid"}],"properties":{"D2DD2v2FYpYQLFwji":{"1ed8ac857097c4fbbbc15cd373ef96bc":"OneShot","0ac71517d2d368cdbabf8b9914716902":"pUC19","e1520b5997a532c7889f6e8883920ab8":"E. coli","e4963ca4d4576f5cb6f8af52e3d038b6":"A"}}}},"finishDate":1403947800,"params":{"a1610acb29b294b95abcdeb4b9fdd74b":{"text":"E. coli OneShot with pUC19","type":"experiment","experiment":"KDXh8d3RnXgWTHMQh","v":0,"product":"Culture"}},"protocol":{"_id":"kxBySpW3SnbWuYMRw","name":"Streak bacteria carrying a plasmid onto an agar plate","v":0}},"x/wEjk3Y9hwqpf4r6iN":{"params":{"63aa3271e10f79baaba8465140e427e9":{"text":"pKH6 (O13)","type":"experiment","v":0,"experiment":"huPFq9XyepadmQfMz","product":"Purified plasmid"}},"protocol":{"name":"Dilute plasmid DNA","v":0,"_id":"ATv6a4HMeQZMa9WYX"},"products":{"a925072cd10bc1a0a6669b05e867cd0b":{"properties":{"cvt43tybpDTWdk2xR":{"c29e15397d29fc94dad70016db7ab2e9":"C"},"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"pKH6","4da843835f15245d46602c3d57b579b6":"1","6f8ac39f837ee4598b7358ce18aec601":"O13-2"}},"text":"pKH6 (O13-2)","type":{"name":"Plasmid","v":4,"_id":"cvt43tybpDTWdk2xR"},"allTypes":[{"_id":"k9LEWTcbmQn53Gzo7","name":"dsDNA","v":3},{"name":"Plasmid","v":4,"_id":"cvt43tybpDTWdk2xR"}]}},"finishDate":1403953200,"values":{"0":{"0":"","2":"1","1":"O13-2"}}},"x/jvDXAvxREGn6LNzce":{"finishDate":1403953200,"products":{"a925072cd10bc1a0a6669b05e867cd0b":{"text":"pSB1C3-BBa_E0240 (O5-2)","type":{"_id":"cvt43tybpDTWdk2xR","name":"Plasmid","v":4},"allTypes":[{"_id":"k9LEWTcbmQn53Gzo7","v":3,"name":"dsDNA"},{"name":"Plasmid","v":4,"_id":"cvt43tybpDTWdk2xR"}],"properties":{"cvt43tybpDTWdk2xR":{"c29e15397d29fc94dad70016db7ab2e9":"C"},"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"pSB1C3-BBa_E0240","6f8ac39f837ee4598b7358ce18aec601":"O5-2","4da843835f15245d46602c3d57b579b6":"1"}}}},"values":{"0":{"0":"","1":"O5-2","2":"1"}},"params":{"63aa3271e10f79baaba8465140e427e9":{"text":"pSB1C3-BBa_E0240 (O5)","type":"experiment","experiment":"eJcYAQyRtDs7p6v9E","v":0,"product":"Purified plasmid"}},"protocol":{"_id":"ATv6a4HMeQZMa9WYX","name":"Dilute plasmid DNA","v":0}},"x/NjuFxo2x67pjSWSJu":{"values":{"0":{"1":"O18-2","2":"1","0":""}},"products":{"a925072cd10bc1a0a6669b05e867cd0b":{"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"pHYRSF1-02","6f8ac39f837ee4598b7358ce18aec601":"O18-2","4da843835f15245d46602c3d57b579b6":"1"},"cvt43tybpDTWdk2xR":{"c29e15397d29fc94dad70016db7ab2e9":"C"}},"type":{"name":"Plasmid","v":4,"_id":"cvt43tybpDTWdk2xR"},"text":"pHYRSF1-02 (O18-2)","allTypes":[{"name":"dsDNA","v":3,"_id":"k9LEWTcbmQn53Gzo7"},{"v":4,"name":"Plasmid","_id":"cvt43tybpDTWdk2xR"}]}},"finishDate":1403953200,"protocol":{"v":0,"name":"Dilute plasmid DNA","_id":"ATv6a4HMeQZMa9WYX"},"params":{"63aa3271e10f79baaba8465140e427e9":{"product":"Purified plasmid","experiment":"9fQJLPSWkzRoJ7P8W","v":0,"text":"pHYRSF1-02 (O18)","type":"experiment"}}},"x/GHLp5ySiA96n633XD":{"protocol":{"v":0,"name":"Dilute plasmid DNA","_id":"ATv6a4HMeQZMa9WYX"},"params":{"63aa3271e10f79baaba8465140e427e9":{"type":"experiment","text":"pHYRSF1 (O17)","experiment":"asHXq4i2fLEskTAuq","v":0,"product":"Purified plasmid"}},"values":{"0":{"0":"","1":"O17-2","2":"1"}},"finishDate":1403953200,"products":{"a925072cd10bc1a0a6669b05e867cd0b":{"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"pHYRSF1","4da843835f15245d46602c3d57b579b6":"1","6f8ac39f837ee4598b7358ce18aec601":"O17-2"},"cvt43tybpDTWdk2xR":{"c29e15397d29fc94dad70016db7ab2e9":"C"}},"text":"pHYRSF1 (O17-2)","type":{"v":4,"name":"Plasmid","_id":"cvt43tybpDTWdk2xR"},"allTypes":[{"name":"dsDNA","v":3,"_id":"k9LEWTcbmQn53Gzo7"},{"_id":"cvt43tybpDTWdk2xR","v":4,"name":"Plasmid"}]}}},"x/cpSEeBbQy7Fm2ERCA":{"products":{"c3aa9e8a1d807a1fafce9630fe0e797b":{"type":{"_id":"k9LEWTcbmQn53Gzo7","v":1,"name":"dsDNA"},"text":"pSB1C3_NpuDnaE-N_FLAG-NpuDnaE-C","allTypes":[{"name":"dsDNA","v":1,"_id":"k9LEWTcbmQn53Gzo7"}],"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"pSB1C3_NpuDnaE-N_FLAG-NpuDnaE-C"}}}},"finishDate":1403958600,"values":{"0":{"0":"19"},"4":{"7":"40","11":"pSB1C3_NpuDnaE-N_FLAG-NpuDnaE-C","2":"98","9":72,"5":"20","1":"30","4":"66","8":"12","0":"98","3":"5","10":300,"6":"72"}},"protocol":{"name":"Phusion Flash PCR ","v":5,"_id":"WSMMH3bg6CcrcAJ3x"},"params":{"5d37875fd1572e9ebc236d502ab9a20d":[{"paramValue":{"product":"Plasmid DNA","v":0,"experiment":"jvDXAvxREGn6LNzce","text":"pSB1C3-BBa_E0240 (O5-2)","type":"experiment"},"values":{"3":{"0":"1"}}}],"dd152b9c02bb725f7af5038f5b1649ac":[{"values":{"2":{"0":"2.5"}},"paramValue":{"supply":"zF2cMKPqTyxeiaQX4","v":0,"type":"supply","text":"pSB1C3_F_NpuDnaE-N (13)"}},{"paramValue":{"text":"pSB1C3_R_FLAG-NpuDnaE-C (14)","type":"supply","supply":"oNTENexnJr757EemM","v":0},"values":{"2":{"0":"2.5"}}}]}},"x/5LAYenuu8CZj33EQr":{"protocol":{"_id":"WSMMH3bg6CcrcAJ3x","name":"Phusion Flash PCR ","v":5},"params":{"5d37875fd1572e9ebc236d502ab9a20d":[{"values":{"3":{"0":"1"}},"paramValue":{"type":"experiment","text":"pHYRSF1-02 (O18-2)","experiment":"NjuFxo2x67pjSWSJu","v":0,"product":"Plasmid DNA"}}],"dd152b9c02bb725f7af5038f5b1649ac":[{"values":{"2":{"0":"2.5"}},"paramValue":{"text":"NpuDnaE-C_F_pSB1C3-FLAG (15)","type":"supply","supply":"uAsXMz5Ln3nXAPnqm","v":0}},{"values":{"2":{"0":"2.5"}},"paramValue":{"type":"supply","text":"NpuDnaE-C_R_6His-BsaI-ccdB (21)","supply":"S3tX3yCPZALuuCu9f","v":0}}]},"finishDate":1403958600,"products":{"c3aa9e8a1d807a1fafce9630fe0e797b":{"allTypes":[{"_id":"k9LEWTcbmQn53Gzo7","name":"dsDNA","v":1}],"text":"NpuDnaE-C_pSB1C3-FLAG_6His-BsaI-ccdB","type":{"v":1,"name":"dsDNA","_id":"k9LEWTcbmQn53Gzo7"},"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"NpuDnaE-C_pSB1C3-FLAG_6His-BsaI-ccdB"}}}},"values":{"0":{"0":"19"},"4":{"2":"98","11":"NpuDnaE-C_pSB1C3-FLAG_6His-BsaI-ccdB","5":"20","9":72,"1":"30","4":"72","8":"10","7":"15","3":"5","10":300,"6":"72","0":"98"}}},"x/dRNY3FKA9bpJxonPE":{"values":{"0":{"0":"19"},"4":{"0":"98","3":"5","6":"72","10":300,"7":"15","9":72,"1":"30","5":"20","2":"98","11":"NpuDnaE-N_BsaI-ccdB_pSB1C3","8":"10","4":"72"}},"finishDate":1403958600,"products":{"c3aa9e8a1d807a1fafce9630fe0e797b":{"allTypes":[{"v":1,"name":"dsDNA","_id":"k9LEWTcbmQn53Gzo7"}],"type":{"name":"dsDNA","v":1,"_id":"k9LEWTcbmQn53Gzo7"},"text":"NpuDnaE-N_BsaI-ccdB_pSB1C3","properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"NpuDnaE-N_BsaI-ccdB_pSB1C3"}}}},"params":{"5d37875fd1572e9ebc236d502ab9a20d":[{"paramValue":{"experiment":"GHLp5ySiA96n633XD","v":0,"type":"experiment","text":"pHYRSF1 (O17-2)","product":"Plasmid DNA"},"values":{"3":{"0":"1"}}}],"dd152b9c02bb725f7af5038f5b1649ac":[{"values":{"2":{"0":"2.5"}},"paramValue":{"text":"NpuDnaE-N_F_BsaI-ccdB (19)","type":"supply","v":0,"supply":"KRd7NmwJTKTYyskCe"}},{"values":{"2":{"0":"2.5"}},"paramValue":{"type":"supply","text":"NpuDnaE-N_R_pSB1C3 (20)","v":0,"supply":"bQ2ct4MmsNhL2keWL"}}]},"protocol":{"name":"Phusion Flash PCR ","v":5,"_id":"WSMMH3bg6CcrcAJ3x"}},"x/2mE2eJKxLMKTrvPK3":{"finishDate":1403958600,"products":{"c3aa9e8a1d807a1fafce9630fe0e797b":{"text":"NpuDnaE-C_pSB1C3-FLAG_BsaI-ccdB","type":{"_id":"k9LEWTcbmQn53Gzo7","v":1,"name":"dsDNA"},"allTypes":[{"name":"dsDNA","v":1,"_id":"k9LEWTcbmQn53Gzo7"}],"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"NpuDnaE-C_pSB1C3-FLAG_BsaI-ccdB"}}}},"values":{"4":{"3":"5","10":300,"6":"72","0":"98","2":"98","11":"NpuDnaE-C_pSB1C3-FLAG_BsaI-ccdB","9":72,"5":"20","1":"30","4":"72","8":"10","7":"15"},"0":{"0":"19"}},"params":{"dd152b9c02bb725f7af5038f5b1649ac":[{"paramValue":{"v":0,"supply":"uAsXMz5Ln3nXAPnqm","text":"NpuDnaE-C_F_pSB1C3-FLAG (15)","type":"supply"},"values":{"2":{"0":"2.5"}}},{"values":{"2":{"0":"2.5"}},"paramValue":{"type":"supply","text":"NpuDnaE-C_R_BsaI-ccdB (16)","supply":"gQpZCbJ6kK8qtAJkf","v":0}}],"5d37875fd1572e9ebc236d502ab9a20d":[{"values":{"3":{"0":"1"}},"paramValue":{"experiment":"NjuFxo2x67pjSWSJu","v":0,"text":"pHYRSF1-02 (O18-2)","type":"experiment","product":"Plasmid DNA"}}]},"protocol":{"v":5,"name":"Phusion Flash PCR ","_id":"WSMMH3bg6CcrcAJ3x"}},"x/D9whLDgLDHrGqvJbZ":{"protocol":{"_id":"JPmjNykwWASQiD9PX","name":"Gel Electrophoresis","v":1},"params":{"5d37875fd1572e9ebc236d502ab9a20d":[{"paramValue":{"text":"GeneRuler 1 kb Plus (Fermentas) ","type":"supply","v":1,"supply":"pKYt3PtA4JPJRis2q"},"values":{"10":{"0":"3.0"}}},{"paramValue":{"experiment":"cpSEeBbQy7Fm2ERCA","v":0,"type":"experiment","text":"pSB1C3_NpuDnaE-N_FLAG-NpuDnaE-C","product":"Amplificate"},"values":{"10":{"0":"10.0"}}},{"paramValue":{"experiment":"5LAYenuu8CZj33EQr","v":0,"text":"NpuDnaE-C_pSB1C3-FLAG_6His-BsaI-ccdB","type":"experiment","product":"Amplificate"},"values":{"10":{"0":"10.0"}}},{"values":{"10":{"0":"10.0"}},"paramValue":{"text":"ccdB_NpuDnaE-C-6His-BsaI_BasI-NpuDnaE-N","type":"experiment","v":0,"experiment":"aG2n4wiCx5JSR7Jv7","product":"Amplificate"}},{"values":{"10":{"0":"10.0"}},"paramValue":{"experiment":"dRNY3FKA9bpJxonPE","v":0,"text":"NpuDnaE-N_BsaI-ccdB_pSB1C3","type":"experiment","product":"Amplificate"}},{"paramValue":{"product":"Amplificate","type":"experiment","text":"NpuDnaE-C_pSB1C3-FLAG_BsaI-ccdB","experiment":"2mE2eJKxLMKTrvPK3","v":0},"values":{"10":{"0":"10.0"}}},{"values":{"10":{"0":"10.0"}},"paramValue":{"text":"ccdB_NpuDnaE-C-BsaI _BasI-NpuDnaE-N","type":"experiment","v":0,"experiment":"mvNL6iYietBwhWXdL","product":"Amplificate"}},{"paramValue":{"v":1,"supply":"pKYt3PtA4JPJRis2q","text":"GeneRuler 1 kb Plus (Fermentas) ","type":"supply"},"values":{"10":{"0":"3.0"}}}]},"products":{"12327177bfe077ccd10daa3d5ca9809c":{"type":{"_id":"QCyBocmukGKrzbkYb","v":0,"name":"Agarose Gel"},"text":"","allTypes":[{"name":"Agarose Gel","v":0,"_id":"QCyBocmukGKrzbkYb"}],"properties":{}}},"finishDate":1403958600,"sid":"5","values":{"11":{"1":"45","0":"100"}}},"x/aG2n4wiCx5JSR7Jv7":{"params":{"dd152b9c02bb725f7af5038f5b1649ac":[{"values":{"2":{"0":"2.5"}},"paramValue":{"supply":"v5ccMpEYE4mhKJnik","v":0,"type":"supply","text":"ccdB_F_NpuDnaE-C-6His-BsaI (22)"}},{"values":{"2":{"0":"2.5"}},"paramValue":{"text":"ccdB_R_BasI-NpuDnaE-N (18)","type":"supply","v":0,"supply":"KRqpwLkrbeowT5uQt"}}],"5d37875fd1572e9ebc236d502ab9a20d":[{"values":{"3":{"0":"1"}},"paramValue":{"product":"Plasmid DNA","experiment":"wEjk3Y9hwqpf4r6iN","v":0,"text":"pKH6 (O13-2)","type":"experiment"}}]},"protocol":{"_id":"WSMMH3bg6CcrcAJ3x","name":"Phusion Flash PCR ","v":5},"finishDate":1403958600,"products":{"c3aa9e8a1d807a1fafce9630fe0e797b":{"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"ccdB_NpuDnaE-C-6His-BsaI_BasI-NpuDnaE-N"}},"allTypes":[{"_id":"k9LEWTcbmQn53Gzo7","name":"dsDNA","v":1}],"text":"ccdB_NpuDnaE-C-6His-BsaI_BasI-NpuDnaE-N","type":{"_id":"k9LEWTcbmQn53Gzo7","v":1,"name":"dsDNA"}}},"values":{"0":{"0":"19"},"4":{"7":"15","5":"20","9":72,"1":"30","2":"98","11":"ccdB_NpuDnaE-C-6His-BsaI_BasI-NpuDnaE-N","8":"10","4":"72","0":"98","3":"5","6":"72","10":300}}},"x/mvNL6iYietBwhWXdL":{"values":{"0":{"0":"19"},"4":{"8":"10","4":"72","9":72,"1":"30","5":"20","11":"ccdB_NpuDnaE-C-BsaI _BasI-NpuDnaE-N","2":"98","7":"15","6":"72","10":300,"3":"5","0":"98"}},"products":{"c3aa9e8a1d807a1fafce9630fe0e797b":{"properties":{"k9LEWTcbmQn53Gzo7":{"49ee3087348e8d44e1feda1917443987":"ccdB_NpuDnaE-C-BsaI _BasI-NpuDnaE-N"}},"type":{"v":1,"name":"dsDNA","_id":"k9LEWTcbmQn53Gzo7"},"text":"ccdB_NpuDnaE-C-BsaI _BasI-NpuDnaE-N","allTypes":[{"_id":"k9LEWTcbmQn53Gzo7","name":"dsDNA","v":1}]}},"finishDate":1403958600,"protocol":{"name":"Phusion Flash PCR ","v":5,"_id":"WSMMH3bg6CcrcAJ3x"},"params":{"dd152b9c02bb725f7af5038f5b1649ac":[{"paramValue":{"supply":"YEHcWxErENfxBaxS4","v":0,"type":"supply","text":"ccdB_F_NpuDnaE-C-BsaI  (17)"},"values":{"2":{"0":"2.5"}}},{"values":{"2":{"0":"2.5"}},"paramValue":{"text":"ccdB_R_BasI-NpuDnaE-N (18)","type":"supply","v":0,"supply":"KRqpwLkrbeowT5uQt"}}],"5d37875fd1572e9ebc236d502ab9a20d":[{"paramValue":{"product":"Plasmid DNA","text":"pKH6 (O13-2)","type":"experiment","v":0,"experiment":"wEjk3Y9hwqpf4r6iN"},"values":{"3":{"0":"1"}}}]}}}