http://2014.igem.org/wiki/index.php?title=Team:Waterloo/Lab_Book&feed=atom&action=history
Team:Waterloo/Lab Book - Revision history
2024-03-28T17:53:34Z
Revision history for this page on the wiki
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Super.suzie at 03:28, 18 October 2014
2014-10-18T03:28:31Z
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<a href="http://2014.igem.org/wiki/index.php?title=Team:Waterloo/Lab_Book&diff=397365&oldid=386315">Show changes</a>
Super.suzie
http://2014.igem.org/wiki/index.php?title=Team:Waterloo/Lab_Book&diff=386315&oldid=prev
Alexanian: no more boilerplate
2014-10-18T02:04:33Z
<p>no more boilerplate</p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Gel purified the inserts and ligated all the three parts together<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Gel purified the inserts and ligated all the three parts together<br></div></td></tr>
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Alexanian
http://2014.igem.org/wiki/index.php?title=Team:Waterloo/Lab_Book&diff=377646&oldid=prev
Jdmanson at 00:48, 18 October 2014
2014-10-18T00:48:56Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="tabcontents"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="tabcontents"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view0"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view0"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h2>CRISPRi</h2></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/e/e2/CRISPRi_1.png"></a><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/e/e2/CRISPRi_1.png"></a><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/5/53/CRISPRi_2.png"></a><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/5/53/CRISPRi_2.png"></a><br></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 284:</td>
<td colspan="2" class="diff-lineno">Line 283:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view1"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view1"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h2>RNAi</h2></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/e/ea/RNAi_1.png"></a><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/e/ea/RNAi_1.png"></a><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/3/32/RNAi_2.png"></a><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/3/32/RNAi_2.png"></a><br></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 319:</td>
<td colspan="2" class="diff-lineno">Line 317:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view2"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline"><h2>YFP</h2></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/1/1d/YFP_1.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><img src="https://static.igem.org/mediawiki/2014/1/1d/YFP_1.png"></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><img src="https://static.igem.org/mediawiki/2014/5/59/YFP_2.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><img src="https://static.igem.org/mediawiki/2014/5/59/YFP_2.png"></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><img src="https://static.igem.org/mediawiki/2014/0/00/YFP_3.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><img src="https://static.igem.org/mediawiki/2014/0/00/YFP_3.png"></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><img src="https://static.igem.org/mediawiki/2014/1/18/YFP_4.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><img src="https://static.igem.org/mediawiki/2014/1/18/YFP_4.png"></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><img src="https://static.igem.org/mediawiki/2014/9/96/YFP_5.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><img src="https://static.igem.org/mediawiki/2014/9/96/YFP_5.png"></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 374:</td>
<td colspan="2" class="diff-lineno">Line 371:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view3"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view3"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h2>CONJUGATION</h2></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/2/28/Conjugation_1.png"></a><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/2/28/Conjugation_1.png"></a><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/0/0a/Conjugation_2.png"></a><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/0/0a/Conjugation_2.png"></a><br></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 393:</td>
<td colspan="2" class="diff-lineno">Line 389:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view4"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view4"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h2>OTHER</h2></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/8/8e/Other_1.png"></a><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/8/8e/Other_1.png"></a><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/7/7d/Other_2.png"></a><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/7/7d/Other_2.png"></a><br></div></td></tr>
</table>
Jdmanson
http://2014.igem.org/wiki/index.php?title=Team:Waterloo/Lab_Book&diff=377163&oldid=prev
Jdmanson at 00:44, 18 October 2014
2014-10-18T00:44:59Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 00:44, 18 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 285:</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view1"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view1"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>RNAi</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>RNAi</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/e/ea/RNAi_1.png"></a><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/e/ea/RNAi_1.png"></a><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/3/32/RNAi_2.png"></a><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/3/32/RNAi_2.png"></a><br></div></td></tr>
</table>
Jdmanson
http://2014.igem.org/wiki/index.php?title=Team:Waterloo/Lab_Book&diff=377026&oldid=prev
Jdmanson at 00:43, 18 October 2014
2014-10-18T00:43:52Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 00:43, 18 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view0"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view0"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>CRISPRi</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>CRISPRi</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/e/e2/CRISPRi_1.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/e/e2/CRISPRi_1.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/5/53/CRISPRi_2.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/5/53/CRISPRi_2.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/d/d6/CRISPR_3.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/d/d6/CRISPR_3.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/f/f9/CRISPR_4.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/f/f9/CRISPR_4.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/1/1d/CRISPR_5.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/1/1d/CRISPR_5.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/5/5c/CRISPRi_6.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/5/5c/CRISPRi_6.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/8/82/CIRSPRi_7.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/8/82/CIRSPRi_7.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/0/0b/CRISPRi_8.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/0/0b/CRISPRi_8.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/3/37/CRISPRi_9.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/3/37/CRISPRi_9.png"></a><br<ins class="diffchange diffchange-inline">></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 6, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 6, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digested pSB1C3 backbone with EcoRI and PstI and gel purified<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digested pSB1C3 backbone with EcoRI and PstI and gel purified<br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>RNAi</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>RNAi</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/e/ea/RNAi_1.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/e/ea/RNAi_1.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/3/32/RNAi_2.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/3/32/RNAi_2.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/a/a5/RNAi_3.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/a/a5/RNAi_3.png"></a><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 10, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 10, 2014</strong><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>YFP</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>YFP</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/1/1d/YFP_1.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/1/1d/YFP_1.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/5/59/YFP_2.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/5/59/YFP_2.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/0/00/YFP_3.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/0/00/YFP_3.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/1/18/YFP_4.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/1/18/YFP_4.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/9/96/YFP_5.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/9/96/YFP_5.png"></a><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 375:</td>
<td colspan="2" class="diff-lineno">Line 376:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view3"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view3"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>CONJUGATION</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>CONJUGATION</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/2/28/Conjugation_1.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/2/28/Conjugation_1.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/0/0a/Conjugation_2.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/0/0a/Conjugation_2.png"></a><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> content 3 <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> content 3 <br></div></td></tr>
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<td colspan="2" class="diff-lineno">Line 395:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view4"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view4"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>OTHER</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>OTHER</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/8/8e/Other_1.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/8/8e/Other_1.png"></a><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/7/7d/Other_2.png" <del class="diffchange diffchange-inline">class="icon floatLeft"/</del>></a><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/7/7d/Other_2.png"></a><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> asdfasdfasdfasdfasgasdgasdg</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> asdfasdfasdfasdfasgasdgasdg</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> asgasdgad</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> asgasdgad</div></td></tr>
</table>
Jdmanson
http://2014.igem.org/wiki/index.php?title=Team:Waterloo/Lab_Book&diff=376022&oldid=prev
Jdmanson at 00:35, 18 October 2014
2014-10-18T00:35:16Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 00:35, 18 October 2014</td>
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<td colspan="2" class="diff-lineno">Line 22:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view0"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view0"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>CRISPRi</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>CRISPRi</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><strong>June 6, 2014</strong><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img src="https://static.igem.org/mediawiki/2014/e/e2/CRISPRi_1.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img src="https://static.igem.org/mediawiki/2014/5/53/CRISPRi_2.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img src="https://static.igem.org/mediawiki/2014/d/d6/CRISPR_3.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img src="https://static.igem.org/mediawiki/2014/f/f9/CRISPR_4.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img src="https://static.igem.org/mediawiki/2014/1/1d/CRISPR_5.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img src="https://static.igem.org/mediawiki/2014/5/5c/CRISPRi_6.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img src="https://static.igem.org/mediawiki/2014/8/82/CIRSPRi_7.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img src="https://static.igem.org/mediawiki/2014/0/0b/CRISPRi_8.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img src="https://static.igem.org/mediawiki/2014/3/37/CRISPRi_9.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><strong>June 6, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digested pSB1C3 backbone with EcoRI and PstI and gel purified<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digested pSB1C3 backbone with EcoRI and PstI and gel purified<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 9, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 9, 2014</strong><br></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 275:</td>
<td colspan="2" class="diff-lineno">Line 284:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view1"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view1"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>RNAi</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>RNAi</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline"><img src="https://static.igem.org/mediawiki/2014/e/ea/RNAi_1.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img src="https://static.igem.org/mediawiki/2014/3/32/RNAi_2.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img src="https://static.igem.org/mediawiki/2014/a/a5/RNAi_3.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 10, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 10, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Received pJB38 integrative plasmid, pKAN, pSPC, pYFP-F, pYFP-R, pRFP-F, and pRFP-R from Dr. Bose, Dr. Bayles, and Dr. Endres from the University of Nebraska, Nebraska, U.S.A in E. coli DH5α. Inoculated colonies into over night liquid cultures for frozen stocks and miniprep<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Received pJB38 integrative plasmid, pKAN, pSPC, pYFP-F, pYFP-R, pRFP-F, and pRFP-R from Dr. Bose, Dr. Bayles, and Dr. Endres from the University of Nebraska, Nebraska, U.S.A in E. coli DH5α. Inoculated colonies into over night liquid cultures for frozen stocks and miniprep<br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>YFP</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>YFP</h2></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <img src="https://static.igem.org/mediawiki/2014/1/1d/YFP_1.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <img src="https://static.igem.org/mediawiki/2014/5/59/YFP_2.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <img src="https://static.igem.org/mediawiki/2014/0/00/YFP_3.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <img src="https://static.igem.org/mediawiki/2014/1/18/YFP_4.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <img src="https://static.igem.org/mediawiki/2014/9/96/YFP_5.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> content 2 <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> content 2 <br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view3"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view3"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>CONJUGATION</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>CONJUGATION</h2></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <img src="https://static.igem.org/mediawiki/2014/2/28/Conjugation_1.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <img src="https://static.igem.org/mediawiki/2014/0/0a/Conjugation_2.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> content 3 <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> content 3 <br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view4"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="anchor" id="view4"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>OTHER</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>OTHER</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>asdfasdfasdfasdfasgasdgasdg</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img src="https://static.igem.org/mediawiki/2014/8/8e/Other_1.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img src="https://static.igem.org/mediawiki/2014/7/7d/Other_2.png" class="icon floatLeft"/></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>asdfasdfasdfasdfasgasdgasdg</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> asgasdgad</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> asgasdgad</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> sgadsga</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> sgadsga</div></td></tr>
</table>
Jdmanson
http://2014.igem.org/wiki/index.php?title=Team:Waterloo/Lab_Book&diff=363966&oldid=prev
Super.suzie at 22:48, 17 October 2014
2014-10-17T22:48:56Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 22:48, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>RNAi</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>RNAi</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <strong><del class="diffchange diffchange-inline">October X</del>, 2014</strong><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <strong><ins class="diffchange diffchange-inline">June 10</ins>, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">TEXT</del><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Received pJB38 integrative plasmid, pKAN, pSPC, pYFP-F, pYFP-R, pRFP-F, and pRFP-R from Dr. Bose, Dr. Bayles, and Dr. Endres from the University of Nebraska, Nebraska, U.S.A in E. coli DH5α. Inoculated colonies into over night liquid cultures for frozen stocks and miniprep</ins><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <strong><del class="diffchange diffchange-inline">October X</del>, 2014</strong><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <strong><ins class="diffchange diffchange-inline">June 16</ins>, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">TEXT</del><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Received synthesized YFP construct (1st half of integration insert) from BioBasic and transformed the DNA into E. coli DH5α</ins><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <strong><del class="diffchange diffchange-inline">October X</del>, 2014</strong><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <strong><ins class="diffchange diffchange-inline">June 18</ins>, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">TEXT</del><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Inoculated 4 colonies of the YFP construct from the transformation into over night liquid culture for frozen stocks and miniprep<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <strong>June 19, 2014</strong><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Minipreped the 4 cultures and did a diagnostic digest before making frozen stocks<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <strong>June 20, 2014</strong><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Received the synthesized 2nd half of the integration insert containing the aadD gene from BioBasic. Transformed DNA into E. coli DH5α<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <strong>June 21, 2014</strong><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Streak purified 4 colonies of aadD containing construct<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <strong>June 22, 2014</strong><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Inoculated the 4 colonies of aadD containing construct into over night liquid cultures for miniprep and frozen stocks<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <strong>June 23, 2014</strong><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Minipreped the 4 cultures and did a diagnostic digest before making frozen stocks</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Digested the following for modified 3A assembly:<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">- YFP construct with EcoRI and SpeI<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">- aadD containing construct with XbaI and SalI<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">- pJB38 backbone with EcoRI and SalI<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Gel purified the inserts and ligated all the three parts together</ins><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> content 1 <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> content 1 <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> content 1 <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> content 1 <br></div></td></tr>
</table>
Super.suzie
http://2014.igem.org/wiki/index.php?title=Team:Waterloo/Lab_Book&diff=363286&oldid=prev
Super.suzie at 22:42, 17 October 2014
2014-10-17T22:42:41Z
<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 22:42, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Inoculated overnight cultures to fresh LB + antibiotic + xylose in triplicate at 1/100 dilution<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Inoculated overnight cultures to fresh LB + antibiotic + xylose in triplicate at 1/100 dilution<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Grew until 0.5-0.6 OD and read fluorescence with plate reader unsuccessful<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Grew until 0.5-0.6 OD and read fluorescence with plate reader unsuccessful<br></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </div></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <div class="anchor" id="view1"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <h2>RNAi</h2></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <p></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>October X, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>October X, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>TEXT<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>TEXT<br></div></td></tr>
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<td colspan="2" class="diff-lineno">Line 282:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>October X, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>October X, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>TEXT<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>TEXT<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </div></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <div class="anchor" id="view1"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h2>RNAi</h2></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <p></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> content 1 <br></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> content 1 <br></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> content 1 <br></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> content 1 <br></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> content 1 <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> content 1 <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> content 1 <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> content 1 <br></div></td></tr>
</table>
Super.suzie
http://2014.igem.org/wiki/index.php?title=Team:Waterloo/Lab_Book&diff=362665&oldid=prev
Super.suzie at 22:35, 17 October 2014
2014-10-17T22:35:53Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 22:35, 17 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 257:</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Transformation of dCas9-ermM-oriV-pSB1A3 into DH5α and DC10B<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Transformation of dCas9-ermM-oriV-pSB1A3 into DH5α and DC10B<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Measure absorbance spectra for dsRed-ermM-oriV<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Measure absorbance spectra for dsRed-ermM-oriV<br></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <strong>October 13, 2014</strong><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Ligation of dCas9-ermM-oriV and pSB1A3<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <strong>October 14, 2014</strong><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Digest for CRISPR assay <br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">co-transformation successful<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Promoter characterization:<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">3 colonies each :xylose promoter-GFP, sarA promoter-GFP, J23101-GFP, GFP, DH5α, xylose promoter-GFP with xylose<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Inoculated from overnight cultures into 5ml LB in 1/100 dilution with antibiotics<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Incubated for 3 hours and read absorbance and fluorescence on plate reader<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Xylose promoter unsuccessful – use fresh xylose next time<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Reinoculated cultures for next day<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <strong>October 15, 2014</strong><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">CRISPR assay<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Inoculated overnight cultures to fresh LB + antibiotic + xylose in triplicate at 1/100 dilution<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Grew until 0.5-0.6 OD and read fluorescence with plate reader unsuccessful<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <strong>October X, 2014</strong><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">TEXT<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <strong>October X, 2014</strong><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">TEXT<br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>October X, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>October X, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>TEXT<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>TEXT<br></div></td></tr>
</table>
Super.suzie
http://2014.igem.org/wiki/index.php?title=Team:Waterloo/Lab_Book&diff=361730&oldid=prev
Super.suzie at 22:25, 17 October 2014
2014-10-17T22:25:13Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 22:25, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digested pSB1C3 backbone with EcoRI and PstI and gel purified<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digested pSB1C3 backbone with EcoRI and PstI and gel purified<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 9, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 9, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Received synthesized sgRNA 1 and sgRNA 2 constructs from BioBasic and transformed the DNA into E. coli DH5α <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Received synthesized sgRNA 1 and sgRNA 2 constructs from BioBasic and transformed the DNA into <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>DH5α <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 11, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 11, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Inoculated sgRNA 1 and 2 colonies from the transformation into over night liquid culture for frozen stocks and miniprep<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Inoculated sgRNA 1 and 2 colonies from the transformation into over night liquid culture for frozen stocks and miniprep<br></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 34:</td>
<td colspan="2" class="diff-lineno">Line 34:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digested pSB1A3 linearized backbone with EcoRI and PstI, then column purified. <br>Ligated pSB1A3 with the sgRNA 1 and 2 (E/P) inserts individually and with both sgRNA 1 (E/S) and sgRNA 2 (X/P) together <br>Ligated pSB1C3 backbone with sgRNA 1 (E/P) and sgRNA 2 (E/P) individually for BioBricks<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digested pSB1A3 linearized backbone with EcoRI and PstI, then column purified. <br>Ligated pSB1A3 with the sgRNA 1 and 2 (E/P) inserts individually and with both sgRNA 1 (E/S) and sgRNA 2 (X/P) together <br>Ligated pSB1C3 backbone with sgRNA 1 (E/P) and sgRNA 2 (E/P) individually for BioBricks<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 20, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 20, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Transformed all ligations into E. coli DH5α<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Transformed all ligations into <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>DH5α<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 21, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>June 21, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Streak purified 4 colonies from each ligation<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Streak purified 4 colonies from each ligation<br></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 58:</td>
<td colspan="2" class="diff-lineno">Line 58:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Miniprepped and sent clones for sequencing<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Miniprepped and sent clones for sequencing<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>July 9, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>July 9, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Received synthesized dCas9 cassette from BioBasic and transformed the DNA into E. coli DH5α<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Received synthesized dCas9 cassette from BioBasic and transformed the DNA into <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>DH5α<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>July 10, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>July 10, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digested the dCas9 construct with EcoRI and PstI then gel purified the insert<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digested the dCas9 construct with EcoRI and PstI then gel purified the insert<br></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 64:</td>
<td colspan="2" class="diff-lineno">Line 64:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ligated the dCas9 cassette (E/P) into pSB1A3 (E/P) and pSB1C3 (E/P)<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ligated the dCas9 cassette (E/P) into pSB1A3 (E/P) and pSB1C3 (E/P)<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>July 11, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>July 11, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Transformed all ligations into E. coli DH5α<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Transformed all ligations into <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>DH5α<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>July 12, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>July 12, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Streak purified 8 colonies from each ligation<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Streak purified 8 colonies from each ligation<br></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 161:</td>
<td colspan="2" class="diff-lineno">Line 161:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Streak purified DsRed-ermM-oriV and sgRNA from stock<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Streak purified DsRed-ermM-oriV and sgRNA from stock<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>September 5, 2014</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <strong>September 5, 2014</strong><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Testing erythromycin resistance in <del class="diffchange diffchange-inline">e</del>. coli <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Testing erythromycin resistance in <ins class="diffchange diffchange-inline"><i>E</ins>. coli<ins class="diffchange diffchange-inline"></i> </ins><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>DsRed-ermM-oriV<sub><i>S. aureus</i></sub> in pUC57, DC10B<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>DsRed-ermM-oriV<sub><i>S. aureus</i></sub> in pUC57, DC10B<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>sgRNA in pUC57, DH5α (negative control)<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>sgRNA in pUC57, DH5α (negative control)<br></div></td></tr>
</table>
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