Team:Waterloo/Acknowledgements

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  <h2><b>We would like to thank everyone involved:</b></h2>
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We would like to acknowledge and extend our heartfelt gratitude to the following people and groups for lending us valuable supports throughout our project year. We could not have accomplished what we have done with your help and guidance.
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<h3>Overall</h3>
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Thank you to the Department of Biology and Faculty of Science from the University of Waterloo for providing us with our new laboratory. We are excited to use it for the next project year!
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<p>Special thanks to Dr. Trevor Charles for continuing to provide the team with laboratory space as we made this transition and for supporting the team since its beginning in the iGEM competition.</p>
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<p>We would also like to thank our faculty advisors; Dr. Brian Ingalls, Dr. Trevor Charles, Dr. Barb Moffatt, Dr. Marc Aucoin and Dr. Andrew Doxey and our grad advisors for helping us with the design of the project and giving us insightful feedback and suggestions.</p>
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<h3>Lab and Design</h3>
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<li style="list-style-type:circle;">All members of the Charles' lab at University of Waterloo, Department of Biology for your continuous support and guidance for the last few years.</li>
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<li style="list-style-type:circle;">Dr. Jeffery Bose from University of Kansas (KUMC), Dr. Kenneth Bayles and Jennifer Endres from University of Nebraska (NUMC) for providing us pJB38 and other related parts, and the sequences of YFP, DsRed, spC and aadD genes. Thank you for also providing guidance to our knowledge of integrating YFP into our <i>S. epidermidis</i> host.</li>
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<li style="list-style-type:circle;">Dr. Timothy Foster from Trinity College in Dublin, Ireland, for providing us with the <i>E. coli</i> strain DC10B that has the appropriate methylation system that can facilitate transformation of <i>Staphylococcus</i>.</li>
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<li style="list-style-type:circle;">Karen Miinch and Gracia Murase for providing us with <i>S. epidermidis</i> strain.</li>
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<li style="list-style-type:circle;">Christopher Randall from Dr Alex O’Neill’s lab at the University of Leeds (U.K.) for pGO1 plasmid and advice on propagating the plasmid in <i>S. aureus</i>.</li>
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<li style="list-style-type:circle;">Dr. Niels Bols, Dr. John Pham, and Dr. Nathan Vo from Bols' lab for helping us using the fluorescent plate reader.</li>
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<li style="list-style-type:circle;">Dr. Niles Patrick Donegan from the Ambrose Lab in the Department of Microbiology & Immunology at The Geisel School of Medicine at Dartmouth for providing the xylose inducible promoter and relaying the corresponding literature.</li>
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<li style="list-style-type:circle;">Dr. Sang Yup Lee from Korean Advanced Institute of Science and Technology (KAIST) for providing us guidance on how to design sRNA constructs.</li>
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<li style="list-style-type:circle;">Dr. Michael Murphy and Angele from University of British Columbia, Department of Microbiology and Immunology for sending us <i>S. aureus</i> strain Newman.
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<h3>Mathematical Modeling</h3>
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  <h3><b> SJAM </b></h3>
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We would like to thank Dr. Brian Ingalls and Dr. Matthew Scott for their outstanding continued support.
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  Waterloo iGEM team visited a local high school, Sir John A. MacDonald Secondary High School, to talk to their Science Club. We introduced them to the world of synthetic biology and the iGEM competition. We also talked about the more recent addition of the high school level iGEM competition and what kind of projects have come out of it, in hopes of inspiring them to pursue this vastly expanding field of science. We ended our visit with a fun activity where we showed students how to transform <i>Eschericia coli</i> and an RFP gene. They then got a chance to create bacterial art using the original and transformed bacteria to see the difference in the gene's expression!
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<h3>Human Practices & Outreach</h3>
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  <h3> <b> Shad Valley </b> </h3>
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  Shad Valley is an initiative focused on enriching the education of exceptional high school students in Canada. To give students a more hands-on experience, our team hosted a small workshop for selected students to perform a bacteria transformation using and RFP gene. This was done over three workshops as the team went through lab safety, general experimental guidelines, guiding students through the experiment, check their results, and a Q&A period.  
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  <h3> <b> YouTube </b> </h3>
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  Waterloo’s iGEM team has come out with a video series on lab techniques to compliment students' practical education in science. It is our hope that this becomes a tool which will give its audience the confidence and knowledge to perform their experiments with better safety and fewer errors
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<li style="list-style-type:circle;">The Quantum Nano Centre at the University of Waterloo for providing an appropriate laboratory space for us to host the Shad Valley workshop.</li>
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<li style="list-style-type:circle;">The Department of Chemistry from the University of Waterloo NE224L course for making available the activities that we used to standardize the Bacterial Transformations workshop.</li>
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<li style="list-style-type:circle;">Dr. Marianna Foldvari from the University of Waterloo School of Pharmacy for direction in understanding the ingredients of a topical medication using bacteria, and offering related literature.</li>
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Latest revision as of 23:26, 21 November 2014

Acknowledgements:

We would like to acknowledge and extend our heartfelt gratitude to the following people and groups for lending us valuable supports throughout our project year. We could not have accomplished what we have done with your help and guidance.

Overall

Thank you to the Department of Biology and Faculty of Science from the University of Waterloo for providing us with our new laboratory. We are excited to use it for the next project year!

Special thanks to Dr. Trevor Charles for continuing to provide the team with laboratory space as we made this transition and for supporting the team since its beginning in the iGEM competition.

We would also like to thank our faculty advisors; Dr. Brian Ingalls, Dr. Trevor Charles, Dr. Barb Moffatt, Dr. Marc Aucoin and Dr. Andrew Doxey and our grad advisors for helping us with the design of the project and giving us insightful feedback and suggestions.

Lab and Design

  • All members of the Charles' lab at University of Waterloo, Department of Biology for your continuous support and guidance for the last few years.
  • Dr. Jeffery Bose from University of Kansas (KUMC), Dr. Kenneth Bayles and Jennifer Endres from University of Nebraska (NUMC) for providing us pJB38 and other related parts, and the sequences of YFP, DsRed, spC and aadD genes. Thank you for also providing guidance to our knowledge of integrating YFP into our S. epidermidis host.
  • Dr. Timothy Foster from Trinity College in Dublin, Ireland, for providing us with the E. coli strain DC10B that has the appropriate methylation system that can facilitate transformation of Staphylococcus.
  • Karen Miinch and Gracia Murase for providing us with S. epidermidis strain.
  • Christopher Randall from Dr Alex O’Neill’s lab at the University of Leeds (U.K.) for pGO1 plasmid and advice on propagating the plasmid in S. aureus.
  • Dr. Niels Bols, Dr. John Pham, and Dr. Nathan Vo from Bols' lab for helping us using the fluorescent plate reader.
  • Dr. Niles Patrick Donegan from the Ambrose Lab in the Department of Microbiology & Immunology at The Geisel School of Medicine at Dartmouth for providing the xylose inducible promoter and relaying the corresponding literature.
  • Dr. Sang Yup Lee from Korean Advanced Institute of Science and Technology (KAIST) for providing us guidance on how to design sRNA constructs.
  • Dr. Michael Murphy and Angele from University of British Columbia, Department of Microbiology and Immunology for sending us S. aureus strain Newman.

    Mathematical Modeling

    We would like to thank Dr. Brian Ingalls and Dr. Matthew Scott for their outstanding continued support.

    Human Practices & Outreach

  • The Quantum Nano Centre at the University of Waterloo for providing an appropriate laboratory space for us to host the Shad Valley workshop.
  • The Department of Chemistry from the University of Waterloo NE224L course for making available the activities that we used to standardize the Bacterial Transformations workshop.
  • Dr. Marianna Foldvari from the University of Waterloo School of Pharmacy for direction in understanding the ingredients of a topical medication using bacteria, and offering related literature.