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From 2014.igem.org

Contents 1 Protocols 1.1 Media, Plates and Solutions 1.1.1 CCMB 1.1.2 LB-Agar 1.1.3 Luria Broth 1.1.4 Super Optimal Broth (S.O.B.) 1.2 Basic Cloning 1.2.1 Polymerase Chain Reactions 1.2.2 Restriction Endonuclease Reaction (Digestion) 1.2.3 Ligation 1.3 Escherichia coli Protocols (XL1-Blue and XL10-Gold) 1.3.1 Competent Cell Culturing 1.3.2 Transformations 1.3.3 Plating 1.3.4 Overnights 1.3.5 DNA-Extraction and mini-preps 1.3.6 Glycerol Stocks 1.4 Saccharomyces cerevisiae (PYE1 Yeast) 1.4.1 Transformations 1.4.2 OVernight Culturing and Passaging 1.4.3 Glycerol Stocks 1.5 Flow Cytometry and Fluorescence Activated Cell Sorting 1.5.1 Dilutions 1.5.2 Final Preparations 1.6 Circluar Dichroism, Thermal Melts and Guandinium Hydrogen Chloride Melts

Protocols

Media, Plates and Solutions

CCMB

LB-Agar

Luria Broth

Super Optimal Broth (S.O.B.)

Basic Cloning

Polymerase Chain Reactions

Restriction Endonuclease Reaction (Digestion)

Ligation

Escherichia coli Protocols (XL1-Blue and XL10-Gold)

Competent Cell Culturing

Transformations

Plating

Overnights

DNA-Extraction and mini-preps

Glycerol Stocks

Saccharomyces cerevisiae (PYE1 Yeast)

Transformations

OVernight Culturing and Passaging

Glycerol Stocks

Flow Cytometry and Fluorescence Activated Cell Sorting

Dilutions

Final Preparations

Circluar Dichroism, Thermal Melts and Guandinium Hydrogen Chloride Melts

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