Team:Washington

From 2014.igem.org

(Difference between revisions)
Line 80: Line 80:
<!--requirements section -->
<!--requirements section -->
-
<tr><td colspan="3"> <h3> Requirements </h3></td></tr>
+
<tr><td colspan="3"> <h3> Overview</h3></td></tr>
<tr>
<tr>
-
<td width="45%" valign="top">  
+
<td style="border:1px solid white; color: #39275B; font-family:'Lucida Sans Unicode', 'Lucida Grande', sans-serif" colspan="3" align="center" height="150px" >
 +
<p> Stabilizing proteins is an incredibly important and time consuming task in the field of protein engineering. Our team has developed a high throughput method to select for the increased expression and stability of engineered proteins, making them more amenable to large-scale production in Escherichia coli and other downstream applications. Our method involves the insertion of proteins into a Gal4-VP16 transactivator that binds a promoter directly upstream of a GFP gene. This allows for the subsequent selection of mutants associated with higher GFP output, correlating to higher stability, using fluorescence-activated cell sorting. Additionally, our method utilizes degrons to alter the dynamic range of this GFP output. This revolutionary method is a potentially generalizable alternative to current, labor intensive approaches for the selection of stable protein variants. Engineered proteins selected through this method could be produced in bacteria and aid in the development of thermostable, de novo protein therapeutics. </p>
 +
</td>
-
<p> Please be sure to keep these links, your audience will want to find your: </p>
 
-
<!-- Links to other team pages -->
 
-
<ul>
 
-
<li><a href="https://2014.igem.org/Team:Washington">Home</a> </li>
 
-
<li><a href="https://2014.igem.org/Team:Washington/Team">Team</a> </li>
 
-
<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Washington">Official Team Profile</a> </li>
 
-
<li><a href="https://2014.igem.org/Team:Washington/Project">Project</a> </li>
 
-
<li><a href="https://2014.igem.org/Team:Washington/Parts">Parts</a> </li>
 
-
<li><a href="https://2014.igem.org/Team:Washington/Modeling">Modeling</a> </li>
 
-
<li><a href="https://2014.igem.org/Team:Washington/Notebook">Notebook</a> </li>
 
-
<li><a href="https://2014.igem.org/Team:Washington/Safety">Safety</a> </li>
 
-
<li><a href="https://2014.igem.org/Team:Washington/Attributions">Attributions</a> </li>
 
-
</ul>
 
-
</td>
 
-
 
-
<td > </td>
 
-
<td width="45%">
 
-
 
-
<p>There are a few wiki requirements teams must follow:</p>
 
-
<ul>
 
-
<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
 
-
<li>All pages must be created under the team’s name space.</li>
 
-
<li>As part of your documentation, keep the links from the menu to the left. </li>
 
-
<li>Do not use flash in wiki code. </li>
 
-
<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
 
-
</ul>
 
-
<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
 
-
 
-
</td>
 
</tr>
</tr>
Line 124: Line 97:
<!--tips  -->
<!--tips  -->
-
<tr><td colspan="3" > <h3> Tips  </h3></td></tr>
+
<tr><td colspan="3" >&nbsp;</td></tr>
<tr>
<tr>
-
<td width="45%" valign="top">  
+
<td width="55%" valign="top">&nbsp;</td>
-
<p>We are currently working on providing teams with some easy to use design templates.
+
-
<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
+
-
<ul>
+
<td width="0%"></td>
-
<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
+
<td width="45%">&nbsp;</td>
-
<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
+
-
<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
+
-
<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
+
-
<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
+
-
</ul>
+
-
 
+
-
<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
+
-
</td>
+
-
 
+
-
<td> </td>
+
-
<td width="45%">  
+
-
 
+
-
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
-
 
+
-
<ul>
+
-
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
-
<li>Be clear about what you are doing and what you plan to do.</li>
+
-
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
-
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
-
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+
-
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
+
-
<li>Have lots of fun! </li>
+
-
</ul>
+
-
</br>
+
-
</td>
+
</tr>
</tr>
</table>
</table>

Revision as of 02:55, 6 September 2014


University of Washington iGEM 2014

Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!


Click here to edit this page!

Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

Overview

Stabilizing proteins is an incredibly important and time consuming task in the field of protein engineering. Our team has developed a high throughput method to select for the increased expression and stability of engineered proteins, making them more amenable to large-scale production in Escherichia coli and other downstream applications. Our method involves the insertion of proteins into a Gal4-VP16 transactivator that binds a promoter directly upstream of a GFP gene. This allows for the subsequent selection of mutants associated with higher GFP output, correlating to higher stability, using fluorescence-activated cell sorting. Additionally, our method utilizes degrons to alter the dynamic range of this GFP output. This revolutionary method is a potentially generalizable alternative to current, labor intensive approaches for the selection of stable protein variants. Engineered proteins selected through this method could be produced in bacteria and aid in the development of thermostable, de novo protein therapeutics.

 
   

Retrieved from "http://2014.igem.org/Team:Washington"