Team:WashU StLouis/Attributions

From 2014.igem.org

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<tr><td > <h3> iGEM Team attributions page</h3></td>
 
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Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster.
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<h3>iGEM team members</h3>
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<div style="margin-left: 40px;">The Brauer Group: Jeffrey worked with
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Benjamin Huang as part of the Brauer Group. They were in charge of the
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light regulation aspect of the WashU iGEM project. Ben and Jeffrey
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spent most of the summer cloning and testing the light regulated
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transcription elements. <br>
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<td > <h3> Why do we have this requirement?</h3></td>
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<br>
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<div style="margin-left: 40px;">Ben also spent a significant amount of
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<td > <h3>Attribution Template </h3></td>  
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time coding and making the team wiki page look pretty awesome. He did
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most of the coding for the site through self-learning of html and often
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sought Google for help involving web design elements. He did not use
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any outside resources or companies to code the site, but borrowed
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inspiration and snippets of code from previous iGEM teams: in
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particular 2013 WLC-Milwaukee, 2013 TMU-Tokyo, and 2012 WashU. He also
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began work on the kinetic model system of the light regulation system.
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<p>
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He continued cloning work during the school year, along with taking over organization of the team and keeping up with the many deadlines when Jeffrey went
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We have this requirement to help the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, just be sure to report the work your team did and the work that was done by others.  
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abroad.<br>
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</p>
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<br>
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Jeffrey worked on managing the team, setting up meetings, and having
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<p>
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conversations with the school administration and other iGEM teams. He
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is something that is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.  
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also worked to create our team apparel.<br>
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</div>
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<br>
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The Rebstock Group: Caroline Focht worked with Richard Li as part of
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A great example of complete attribution comes from the <a href="https://2011.igem.org/Team:Imperial_College_London/Team">Imperial College London 2011 team</a> (scroll down to the bottom of their team page to see attributions).  
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the Rebstock Group. They were in charge of the plasmid design and Nif
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activity testing aspect of the WashU iGEM project. Caroline and Richard
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spent most of their time in summer in protocol development and nitrogen
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fixation activity testing. They continued running experiments and
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biobricked during the school year.<br>
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<br>
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<div style="margin-left: 40px;">Caroline also concentrated on WashU
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Here are some of the fields we recommend you have on this page. If there are other areas not listed below, but applicable to your team/project, please feel free to also list them on your attributions page. Please feel free to remove any areas not applicable to your project.  
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iGEM Outreach program, which produced numerous educational videos, and
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sought out other iGEM teams for collaborations. She also produced most
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of our logos and graphics for the website and presentations.<br>
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<ol>
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<br>
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<li>General Support</li>
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Richard was lab manger of iGEM at Washington University in St. Louis,
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thus in addition to the wet lab work he was also in charge of
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<li>Project support and advice</li>
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fundraising and securing for the project. <br>
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</div>
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<li>Fundraising help and advice</li>
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</div>
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<br>
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<li>Lab support</li>
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<div style="text-align: center;">
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<h3>Attributions:</h3>
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<li>Difficult technique support</li>
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</div>
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<span style="text-decoration: underline; font-style: italic;">Project
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<li>Project advisor support</li>
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Support:</span><br>
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<br>
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<li>Wiki support</li>
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<div style="margin-left: 40px;">Dr. Himadri Pakrasi and Dr. Tae Seok
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Moon served as mentors during our iGEM project. We had a few meetings
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<li>Presentation coaching</li>
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with them in order to present our project proposals and check on our
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progress updates, and helped to give us feedback and troubleshoot when
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<li>Policy & Practices support</li>
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necessary. They provided the funding for lab supplies.<br>
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<br>
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team.</li>
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<div style="margin-left: 40px;">Dr. Pakrasi: Dr. Pakrasi is Myron and
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Sonya Glassberg/Albert and Blanche Greensfelder Distinguished
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University Professor, also Director of International Center for
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Advanced Renewable Energy and Sustainability (I-CARES). His research
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interests are: Photosynthesis, Nitrogen Fixation, Systems and Synthetic
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Biology. Pakrasi Lab’s current focus is on Bioenergy Production in
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Cyanobacteria.<br>
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<br>
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Dr. Tae Seok Moon: Dr. Moon is a Principal Investigator and Assistant
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Professor in the Department of Energy, Environmental &amp; Chemical
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Engineering (EECE) at Washington University in St. Louis and heads the
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Moon Research Group (MRG). He generously allowed Ben and Jeffrey
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unfettered access to his lab and material equipment at all hours of the
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day (and night). Dr. Moon also financed ordering of primers and DNA
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sequence verification.<br>
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</div>
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<br>
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Our team would not be complete without our list of direct advisors:
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Andrew Ng, Bert Berla, Cheryl Immethun, and Dr. Deng Liu.<br>
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<br>
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<div style="margin-left: 40px;">Andrew Ng:&nbsp; Andrew is an
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undergraduate biomedical engineer interested in the applications of
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systems and synthetic biology to cyanobacteria. He was also a past
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participant in iGEM at WashU. As the major peer advisor for iGEM
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project this year, Andrew provided invaluable advice and guidance on
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all aspects of both Rebstock and Brauer Group’s work, ranging from
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protocol design and lab technique training to providing material
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support for the GC activity testing. <br>
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<br>
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Bertram Berla:&nbsp; Bert is a PhD candidate in Energy, Environmental,
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and Chemical Engineering. He is working to apply techniques developed
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to optimize the operation of chemical reactors to get more useful stuff
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out of our favorite micro-reactors: cyanobacteria. Bert assisted
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Richard and Caroline on experimental design. <br>
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<br>
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Cheryl Immethun: Ms. Immethun is currently a PhD candidate in the MRG
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with Dr. Moon in the EECE department at Washington University in St.
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Louis. She serves as a senior iGEM advisor and over the course of the
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summer, provided invaluable advice and guidance on all aspects of the
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Brauer Group’s work, ranging from primer design and DNA cloning issues
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to providing material support for the light experiment. She also aided
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with cloning during the school year closer to the deadline as work
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conflicts slowed down progress.<br>
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<br>
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Deng Liu: Deng is interested in the research on cyanobacteria,
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especially on the synthetic biology and transcriptional regulation in
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cyanobacteria. Deng helped iGEM members on details of experimental
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design, plasmid design, and cell culturing.<br>
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</div>
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<br>
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<br>
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Our Non-iGEM affiliated advisors worked in their respective labs and
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would help us on a daily basis, whether it giving valuable input on
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experimental or cloning protocols or feedback during our project
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presentations. The list includes:<br>
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<br>
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<div style="margin-left: 40px;">Allie Hoynes-O’Connor, Ray Henson, Ryan
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Lee , and Tatenda Shopera: The graduate students of the MRG helped the
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Brauer Group by aiding in answering every day questions and making sure
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we contributed to the well-being and material readiness of the lab as
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well as following all safety and labeling protocols. Ms.
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Hoynes-O’Connor served as our lab safety officer and gave us guidance
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and training on lab safety procedures. Mr. Henson also gave guidance
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and plasmid material in the Brauer Group’s early attempts at
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constructing a sequence verifiable chromophore. <br>
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<br>
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Kenneth Ng and Soo Ji Kim: Kenneth and Soo Ji are laboratory technician
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staff in the MRG and helped resupply the lab’s vital operating stock
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materials and equipment in addition to giving advice and answering
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questions the iGEM students had. Kenneth provided his advanced
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expertise in adjusting and optimizing protocols drawn from his vast
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knowledge due to a highly inquisitive nature. <br>
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<br>
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Nancy Duan: Nancy is the technician in the Rebstock lab. She takes
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responsibilities of maintaining cyanobacteria culture and assisting
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iGEM in doing experiments such as PCR, sub-cloning, plasmid preparation
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etc. <br>
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<br>
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Jingjie Yu: Jingjie works on developing a new cyanobacteria model
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system for synthetic biology purpose, especially, for biofuel
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production. During iGEM project, she provided detailed advice on nearly
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all aspect of experiment for Rebstock group.<br>
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</div>
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<br>
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At Monsanto, a group of scientists specializing in nitrogen fixation
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sat in on our team’s presentation about the outlook of our nitrogen
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fixation project and offered plenty of suggestions through the advisory
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panel meeting. The diagnostic panel meeting with scientists of Monsanto
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later proved to be productive for the design and procedure of the lab
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work.<br>
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</div>
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<br>
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<span style="font-style: italic; text-decoration: underline;">Modeling
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Support:</span><br>
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<br>
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<div style="margin-left: 40px;">Andrew Ng: Andrew also helped to create
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the list of kinetic equations for our model. <br>
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<br>
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Brandon Nicklas: Brandon Nicklas is an undergraduate at Penn State; he
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helped us to design a kinetic model for our light regulation system. Unfortunately we did not continue with the model as we were missing some experimental values that we ran out of time to find. <br>
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<br>
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Rajib Saha: Rajib is a PhD candidate at Penn State; he worked as a
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liaison between Penn State and WashU and guided Brandon and gave us
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feedback on the modeling.<br>
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</div>
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<br>
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<span style="text-decoration: underline; font-style: italic;">Human
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Practices Support:</span><br>
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<br>
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<div style="margin-left: 40px;">Beyond the support of lab work design,
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Monsanto, and in particular Jose Prado, project lead of a paper
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published about how projects go from concept to market, provided human
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resources and materials including data sets, Power-point slides and
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presentations to enhance WashU’s iGEM video series.<br>
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</div>
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<br>
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<span style="text-decoration: underline; font-style: italic;">Financial
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&amp; Lab Facilities:</span><br>
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<br>
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<div style="margin-left: 40px;">Pakrasi Group: Dr. Pakrasi and his
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research group at Washington University in St. Louis provided material
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and working space support for both Rebstock and Brauer Group. <br>
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<br>
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Moon Group: Dr. Moon and his research group provided material and
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working space support for the Brauer group.<br>
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<br>
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Zhang Group: Dr. Zhang and his research group at Washington University
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in St. Louis provided material support when we were lacking certain
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chemicals and let us use their temperature controlled growth chamber in
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which we conducted our light regulation experiments. <br>
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<br>
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Sigma-Aldrich has supported WashU iGEM program since 2012. This year,
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Tonya Jackson, the Product Manager of Genomics and Research, provided
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help by not only offering funding for our project, gave us feedback on
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our presentation, and also provided us a sample of a new prototype
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media “EnPresso B” to test and enhance growth of our colonies.<br>
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<br>
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Monsanto’s sponsorship continued in 2014 through material and
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advisorial means. On Wednesday, June 4, our team was invited to visit
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Monsanto Headquarters and its affiliated facilities. The tour gave team
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members a terrific impression about industrial-sized biotechnology
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development procedure and growth capabilities.<br>
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<br>
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WashU iGEM would like to thank <a href="http://artsci.wustl.edu">Washington
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University School of Arts &amp; Sciences</a>, <a
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href="http://engineering.wustl.edu">Washington University School of
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Engineering</a>, <a href="http://undergradresearch.wustl.edu">Washington
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University Office of Undergraduate Research</a>, <a
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href="http://icares.wustl.edu/Pages/Home.aspx">Washington University
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International Center for Advanced Renewable Energy and Sustainability</a>,
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<a href="http://www.sigmaaldrich.com/united-states.html">Sigma-Aldrich</a>
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and <a href="http://www.monsanto.com/pages/default.aspx">Monsanto</a>
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for providing funding for stipends and materials. Lastly, we would like
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to thank <a href="http://www.snapgene.com">SnapGene</a> for their
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software to aid our plasmid designs. <br>
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</div>
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Latest revision as of 03:17, 18 October 2014