Team:Wageningen UR

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<h1><b>Saving the bananas</b></h1>
<h1><b>Saving the bananas</b></h1>
 
 
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<p> Every time you go to the supermarket or to the local market to buy bananas you take for granted that there will be bananas readily available for you to enjoy and savour. However, banana plants are under threat and a world without bananas is more plausible than we all might imagine. Banana plants are threatened by <i>Fusarium oxysporum</i>, a soil pathogen that affects plants entering through its root system causing its death. Bananas, apart from being a delicious fruit, have economic importance in many tropical regions. These are the reasons why the <b>iGEM team Wageningen 2014</b> has designed and created BananaGuard, a genetic system in <i>Pseudomonas putida</i> which is able to detect the presence of <i>Fusarium</i> in the soil and produce a combination of antifungals that will inhibit the growth of the pathogen. If you want to read more about the system and our work during the last six months, the <b>iGEM team Wageningen 2014</b> invites you to continue surfing around our Wiki.</p>
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<p> Every time you go to the supermarket or to the local market to buy bananas you take for granted that there will be bananas readily available for you to enjoy and savour. However, banana plants are under threat and a world without bananas is more plausible than we all might imagine. Banana plants are threatened by <i>Fusarium oxysporum</i>. This pathogenic soil fungus enters plants through their root systems and lives off of them until they die. This fungus currently threatens all banana plants worldwide. Bananas, apart from being a delicious fruit, have economic importance in many tropical regions and are an important part of the diet. These are the reasons why the <b>iGEM team Wageningen 2014</b> has designed and created BananaGuard, a genetic system in <i>Pseudomonas putida</i> that detects the presence of <i>F. oxysporum</i> in the soil and produces a combination of antifungals to remove the fungal threat. If you want to read more about the system and our work during the last six months, the <b>iGEM team Wageningen 2014</b> invites you to continue surfing around our Wiki.</p>
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<figure>
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<video width="720" height="405" controls="" style="display:block;margin-left:auto;margin-right:auto;">
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  <source src="https://static.igem.org/mediawiki/2014/c/c3/Wageningen_UR_synenergene_movie.mp4" type="video/mp4">
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  <source src="https://static.igem.org/mediawiki/2014/2/20/Wageningen_UR_synenergene_movie.mov" type="video/mov">
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  <source src="https://static.igem.org/mediawiki/2014/0/08/Wageningen_UR_synenergene_movie.ogg" type="video/ogg">
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  <p>Your browser does not support this video. Please <a href="http://youtu.be/grzNW8j1nrs">watch it on YouTube</a> or upgrade your browser.</p>
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</video>
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<figcaption>Visualisation of BananaGuard. <a href="http://youtu.be/grzNW8j1nrs" class="soft_link">Watch this video on youtube.</a></figcaption>
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</figure>
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<h1>Achievements</h1>
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<ul>
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<h1>Going for Gold</h1>
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<li>We documented and submited  <b>27</b> <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/overview/parts" target="blank">parts</a> to the iGEM Registry.</li>
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<p>As an iGEM team we worked very hard for the past couple of months with the medal requirements in mind. Below is a list of why we deserve gold this year.</p>
<br/>
<br/>
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<li>We validated our own Biobrick parts: Fusaric acid promoter  <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493000"target="blank">(Bba_K1493000)</a> and Kill switch genes and promoters.(link to registry or page?)</li>
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                            <div style="padding-left:20px">
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<img src="https://static.igem.org/mediawiki/2014/b/be/Wageningen_UR_bronze_banana_medal.png" style="float:left;margin-right:10px;"/><h3 style="font-size:20px;padding-top:12px;">Bronze</h3>
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<br/>
<br/>
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<li>We characterized Fusaric acid dependent promoter by measuring GFP fluorescence at different fusaric acid concentrations and Kill switch genes by measuring RFP and GFP fluorescence at different rhammnose concentrations</li>
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<ul style="list-style-image:url('https://static.igem.org/mediawiki/2014/c/c8/Wageningen_UR_check_sign.png')">
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<li>Registered for iGEM</li>
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<li>Completed judging form</li>
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<li>Have a team Wiki</li>
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<li>Present a poster and talk at the iGEM jamboree</li>
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<li>Documented attributions</li>
 +
<li>Documented and submitted <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/overview/parts" target="blank"> <b>27</b> parts</a> to the iGEM Registry</li>
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<li>Met criteria for bronze</li>
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</ul>
<br/>
<br/>
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<li>We considered safety in our project by implementing kill switch system and a system to prevent horizontal gene transfer.</li>
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<img src="https://static.igem.org/mediawiki/2014/c/c0/Wageningen_UR_silver_banana_medal.png" style="float:left;margin-right:10px;" /><h3 style="font-size:20px;padding-top:3px;">Silver</h3>
<br/>
<br/>
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<li>In collaboration with Synenergene we discussed many safety-concerning topics in synthetic biology and created techno-moral scenarios based on BananaGuard project.</li>
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<ul style="list-style-image:url('https://static.igem.org/mediawiki/2014/c/c8/Wageningen_UR_check_sign.png')">
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<li>Validated and submitted the following Biobrick parts from our own project: Fusaric acid dependent promoter <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493000"target="blank">(Bba_K1493000)</a>, <i>pfri</i> (<a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493200"target="blank">Bba_K1493200</a>) and Kill switch genes and promoters (<a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493801"target="blank">Bba_K1493801</a>, <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493802"target="blank">Bba_K1493802</a>, <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493804"target="blank">Bba_K1493804</a>, <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493805"target="blank">Bba_K1493805</a> and <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493806"target="blank">Bba_K1493806</a>) </li>
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<li>Characterized the following Biobrick parts from our own project: Fusaric acid dependent promoter <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493000"target="blank">(Bba_K1493000)</a>, <i>pfri</i> (<a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493200"target="blank">Bba_K1493200</a>) </li>
 +
 +
<li>Considered safety in our project by implementing a <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/project/kill-switch"target="blank">Kill-switch</a> system and a system to prevent <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/project/gene_transfer"target="blank">horizontal gene transfer</a></li>
 +
 +
<li>Collaborated with Synenergene and discussed many safety-concerning topics in synthetic biology and created techno-moral vignettes based on our project (see <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/outreach/synenergene"target="blank">Synenergene</a>).</li>
 +
<li>Met all criteria for silver</li>
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</ul>
<br/>
<br/>
-
<li>We improved the functions of existing BioBrick parts (<a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493504"target="blank">BBa_K1493504</a>, <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493501"target="blank">BBa_K1493501</a>) by an extensive characterization of two promoters in the registry.</li>
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<img src="https://static.igem.org/mediawiki/2014/f/f3/Wageningen_UR_gold_banana_medal.png" style="float:left;margin-right:10px;" /><h3 style="font-size:20px;padding-top:3px;">Gold</h3>
<br/>
<br/>
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<li>We successfully collaborated with <a class="soft_link" href="https://2014.igem.org/Team:TU_Delft-Leiden"target="blank">TU_Delft-Leiden</a> iGEM from the Netherlands by quantifying the modified biofilm, formed in E.coli (see <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/outreach/collaboration"target="blank">collaboration</a>).</li>
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<ul style="list-style-image:url('https://static.igem.org/mediawiki/2014/c/c8/Wageningen_UR_check_sign.png')">
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<br/>
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<li>Improved the functions of existing BioBrick promoter parts (<a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493504"target="blank">BBa_K1493504</a>, <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493501"target="blank">BBa_K1493501</a>) by an <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/project/characterization"target="blank">extensive characterization</a></li>
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<li>We organized a National conference where we invited Dutch iGEM teams and anticipated a collaboration with the Dutch Institute of Health and Environment (RIVM). In September a symposium and a panel session was organized to inform government officials about the risks and possibilities of synthetic biology, sparking the public discussion about the use of synthetic biology (see <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/outreach/policy"target="blank">policy</a>).</li>
+
-
<br/>
+
<li>Collaborated with iGEM <a class="soft_link" href="https://2014.igem.org/Team:TU_Delft-Leiden"target="blank">TU_Delft-Leiden</a> from the Netherlands by quantifying biofilm production formed in <i>E. coli</i> (see <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/outreach/collaboration"target="blank">collaboration</a>)</li>
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<li>To spread a word about our research and to improve the product application, we interviewed and discussed our project with the leading banana plant scientists at Wageningen University, contacted banana engineer from Canarian islands, stakeholder from Philippines and talked to consumers whose everyday diet includes bananas (see <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/outreach/interviews"target="blank">interview</a>). </li>
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-
 
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<li>Organized the first Dutch National meetup with Dutch iGEM teams and laid a foundation for collaboration with the Dutch Institute of Health and Environment (RIVM) (see <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/outreach/meetup"target="blank">National meet-up</a>)</li>
-
</ul>
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-
<p>For more details click <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/overview/achievements"target="blank">here</a></p>
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<li>A symposium and panel session was organized with to inform government officials about the risks and possibilities of synthetic biology, sparking the public debate about the use of synthetic biology (see <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/outreach/policy"target="blank">policy</a>)</li>
-
 
+
 +
<li>Interviewed and discussed our project with panama disease specialists and stakeholders (see <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/outreach/interviews"target="blank">interview</a>)</li>
 +
<li>Participated in <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/project/interlab"target="blank">interlab study</a></li>
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<li>Met all criteria for gold</li>
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</ul>
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<p><b>For more details check out our <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/overview/achievements"target="blank">achievements</a> page!</b></p>
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<h1>Sponsors</h1>
<h1>Sponsors</h1>
<div class="sponsor_box">
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<a class="sponsor_rectangle" href="http://www.wageningenur.nl/en.htm"><img src="https://static.igem.org/mediawiki/2014/6/63/Wageningen_UR_wur_logo.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.wageningenur.nl/en/Benefactors/Service/About-Wageningen-University-Fund.htm" target="blank"><img src="https://static.igem.org/mediawiki/2014/6/6f/Wageningen_UR_WUF_EN.png" style="width:150px"></a>
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<a class="sponsor_rectangle" href="http://www.erasynbio.eu/"><img src="https://static.igem.org/mediawiki/2014/e/ea/Wageningen_UR_erasynbio.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.erasynbio.eu/" target="blank"><img src="https://static.igem.org/mediawiki/2014/e/ea/Wageningen_UR_erasynbio.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.synenergene.eu/"><img src="https://static.igem.org/mediawiki/2014/4/48/Wageningen_UR_synenergene.png"></a>
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<a class="sponsor_rectangle" href="http://www.synenergene.eu/" target="blank"><img src="https://static.igem.org/mediawiki/2014/4/48/Wageningen_UR_synenergene.png"></a>
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<a class="sponsor_rectangle" href="http://www.snapgene.com/"><img src="https://static.igem.org/mediawiki/2014/f/f0/Wageningen_UR_snapgene.png"></a>
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<a class="sponsor_rectangle" href="http://www.snapgene.com/" target="blank"><img src="https://static.igem.org/mediawiki/2014/f/f0/Wageningen_UR_snapgene.png"></a>
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<a class="sponsor_rectangle" href="http://www.sigmaaldrich.com/sigma-aldrich/home.html"><img src="https://static.igem.org/mediawiki/2014/6/61/Wageningen_UR_sigma.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.sigmaaldrich.com/sigma-aldrich/home.html" target="blank"><img src="https://static.igem.org/mediawiki/2014/6/61/Wageningen_UR_sigma.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.gilson.com/"><img src="https://static.igem.org/mediawiki/2014/b/be/Wageningen_UR_gilson.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.gilson.com/" target="blank"><img src="https://static.igem.org/mediawiki/2014/b/be/Wageningen_UR_gilson.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.neb.com/"><img src="https://static.igem.org/mediawiki/2014/d/da/Wageningen_UR_neb.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.neb.com/" target="blank"><img src="https://static.igem.org/mediawiki/2014/d/da/Wageningen_UR_neb.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.dsm.com/corporate/home.html"><img src="https://static.igem.org/mediawiki/2014/9/9f/Wageningen_UR_dsm.png"></a>
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<a class="sponsor_rectangle" href="http://www.dsm.com/corporate/home.html" target="blank"><img src="https://static.igem.org/mediawiki/2014/9/9f/Wageningen_UR_dsm.png"></a>
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<a class="sponsor_wur" href="http://www.wageningenur.nl/en.htm"><img src="https://static.igem.org/mediawiki/2014/8/84/Wageningen_UR_sponsor_departements.jpg"></a>
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<a class="sponsor_wur" href="http://www.wageningenur.nl/en.htm" target="blank"><img src="https://static.igem.org/mediawiki/2014/6/6d/Wageningen_UR_sponsor_departements1.jpg"></a>
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<a class="sponsor_square" href="http://www.vlaggraduateschool.nl/"><img src="https://static.igem.org/mediawiki/2014/8/85/Wageningen_UR_vlag.jpg"></a>
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<a class="sponsor_square" href="http://www.vlaggraduateschool.nl/" target="blank"><img src="https://static.igem.org/mediawiki/2014/8/85/Wageningen_UR_vlag.jpg"></a>
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<a class="sponsor_square" href="http://www.be-basic.org/"><img src="https://static.igem.org/mediawiki/2014/c/c5/Wageningen_UR_be-basic.png"></a>
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<a class="sponsor_square" href="http://www.be-basic.org/" target="blank"><img src="https://static.igem.org/mediawiki/2014/c/c5/Wageningen_UR_be-basic.png"></a>
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Latest revision as of 02:50, 18 October 2014

Wageningen UR iGEM 2014

 

 

 

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visits


Saving the bananas

Every time you go to the supermarket or to the local market to buy bananas you take for granted that there will be bananas readily available for you to enjoy and savour. However, banana plants are under threat and a world without bananas is more plausible than we all might imagine. Banana plants are threatened by Fusarium oxysporum. This pathogenic soil fungus enters plants through their root systems and lives off of them until they die. This fungus currently threatens all banana plants worldwide. Bananas, apart from being a delicious fruit, have economic importance in many tropical regions and are an important part of the diet. These are the reasons why the iGEM team Wageningen 2014 has designed and created BananaGuard, a genetic system in Pseudomonas putida that detects the presence of F. oxysporum in the soil and produces a combination of antifungals to remove the fungal threat. If you want to read more about the system and our work during the last six months, the iGEM team Wageningen 2014 invites you to continue surfing around our Wiki.


Visualisation of BananaGuard. Watch this video on youtube.

Going for Gold

As an iGEM team we worked very hard for the past couple of months with the medal requirements in mind. Below is a list of why we deserve gold this year.


Bronze


  • Registered for iGEM
  • Completed judging form
  • Have a team Wiki
  • Present a poster and talk at the iGEM jamboree
  • Documented attributions
  • Documented and submitted 27 parts to the iGEM Registry
  • Met criteria for bronze

Silver



Gold


  • Improved the functions of existing BioBrick promoter parts (BBa_K1493504, BBa_K1493501) by an extensive characterization
  • Collaborated with iGEM TU_Delft-Leiden from the Netherlands by quantifying biofilm production formed in E. coli (see collaboration)
  • Organized the first Dutch National meetup with Dutch iGEM teams and laid a foundation for collaboration with the Dutch Institute of Health and Environment (RIVM) (see National meet-up)
  • A symposium and panel session was organized with to inform government officials about the risks and possibilities of synthetic biology, sparking the public debate about the use of synthetic biology (see policy)
  • Interviewed and discussed our project with panama disease specialists and stakeholders (see interview)
  • Participated in interlab study
  • Met all criteria for gold

For more details check out our achievements page!



Sponsors

Retrieved from "http://2014.igem.org/Team:Wageningen_UR"