Team:Wageningen UR

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<p>Pathogens belonging to the Fursarium species are known to infect a wide range of crops and cause large losses in agriculture. In this project, we will concentrate on protecting bananas from Fusarium oxysporum f.sp. cubense using an engineered strain of the native soil bacterium Pseudomonas putida. At present commercially grown bananas for export are all the same species, known as Cavendish. Prior to the 1960s, though, the Gros Michel cultivar was the primary banana for export. This species was almost completely wiped out within a decade by the Panama disease that is caused by Fusarium. Recently a new strain of Fusarium has been infecting Cavendish plants and is spreading throughout the world.</p>
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<h1><b>Saving the bananas</b></h1>
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<p> Every time you go to the supermarket or to the local market to buy bananas you take for granted that there will be bananas readily available for you to enjoy and savour. However, banana plants are under threat and a world without bananas is more plausible than we all might imagine. Banana plants are threatened by <i>Fusarium oxysporum</i>. This pathogenic soil fungus enters plants through their root systems and lives off of them until they die. This fungus currently threatens all banana plants worldwide. Bananas, apart from being a delicious fruit, have economic importance in many tropical regions and are an important part of the diet. These are the reasons why the <b>iGEM team Wageningen 2014</b> has designed and created BananaGuard, a genetic system in <i>Pseudomonas putida</i> that detects the presence of <i>F. oxysporum</i> in the soil and produces a combination of antifungals to remove the fungal threat. If you want to read more about the system and our work during the last six months, the <b>iGEM team Wageningen 2014</b> invites you to continue surfing around our Wiki.</p>
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<figcaption>Visualisation of BananaGuard. <a href="http://youtu.be/grzNW8j1nrs" class="soft_link">Watch this video on youtube.</a></figcaption>
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<h1>Going for Gold</h1>
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<p>As an iGEM team we worked very hard for the past couple of months with the medal requirements in mind. Below is a list of why we deserve gold this year.</p>
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                            <div style="padding-left:20px">
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<img src="https://static.igem.org/mediawiki/2014/b/be/Wageningen_UR_bronze_banana_medal.png" style="float:left;margin-right:10px;"/><h3 style="font-size:20px;padding-top:12px;">Bronze</h3>
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<ul style="list-style-image:url('https://static.igem.org/mediawiki/2014/c/c8/Wageningen_UR_check_sign.png')">
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<li>Registered for iGEM</li>
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<li>Completed judging form</li>
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<li>Have a team Wiki</li>
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<li>Present a poster and talk at the iGEM jamboree</li>
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<li>Documented attributions</li>
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<li>Documented and submitted <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/overview/parts" target="blank"> <b>27</b> parts</a> to the iGEM Registry</li>
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<li>Met criteria for bronze</li>
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<p>The first and perhaps most important part is the production of fungal growth inhibitors. In order to prevent these fungal inhibitors from damaging the soil microbiome, their production will be coupled to the sensing of fusaric acid, a characteristic toxin produced by Fusarium. To help our host compete against the fungus, resistance against fusaric acid will also be incorporated.</p>
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<img src="https://static.igem.org/mediawiki/2014/c/c0/Wageningen_UR_silver_banana_medal.png" style="float:left;margin-right:10px;" /><h3 style="font-size:20px;padding-top:3px;">Silver</h3>
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<li>Validated and submitted the following Biobrick parts from our own project: Fusaric acid dependent promoter <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493000"target="blank">(Bba_K1493000)</a>, <i>pfri</i> (<a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493200"target="blank">Bba_K1493200</a>) and Kill switch genes and promoters (<a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493801"target="blank">Bba_K1493801</a>, <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493802"target="blank">Bba_K1493802</a>, <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493804"target="blank">Bba_K1493804</a>, <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493805"target="blank">Bba_K1493805</a> and <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493806"target="blank">Bba_K1493806</a>) </li>
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<li>Characterized the following Biobrick parts from our own project: Fusaric acid dependent promoter <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493000"target="blank">(Bba_K1493000)</a>, <i>pfri</i> (<a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493200"target="blank">Bba_K1493200</a>) </li>
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<li>Considered safety in our project by implementing a <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/project/kill-switch"target="blank">Kill-switch</a> system and a system to prevent <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/project/gene_transfer"target="blank">horizontal gene transfer</a></li>
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<li>Collaborated with Synenergene and discussed many safety-concerning topics in synthetic biology and created techno-moral vignettes based on our project (see <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/outreach/synenergene"target="blank">Synenergene</a>).</li>
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<li>Met all criteria for silver</li>
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</ul>
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<br/>
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<img src="https://static.igem.org/mediawiki/2014/f/f3/Wageningen_UR_gold_banana_medal.png" style="float:left;margin-right:10px;" /><h3 style="font-size:20px;padding-top:3px;">Gold</h3>
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<br/>
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<ul style="list-style-image:url('https://static.igem.org/mediawiki/2014/c/c8/Wageningen_UR_check_sign.png')">
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<li>Improved the functions of existing BioBrick promoter parts (<a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493504"target="blank">BBa_K1493504</a>, <a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493501"target="blank">BBa_K1493501</a>) by an <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/project/characterization"target="blank">extensive characterization</a></li>
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<li>Collaborated with iGEM <a class="soft_link" href="https://2014.igem.org/Team:TU_Delft-Leiden"target="blank">TU_Delft-Leiden</a> from the Netherlands by quantifying biofilm production formed in <i>E. coli</i> (see <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/outreach/collaboration"target="blank">collaboration</a>)</li>
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<li>Organized the first Dutch National meetup with Dutch iGEM teams and laid a foundation for collaboration with the Dutch Institute of Health and Environment (RIVM) (see <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/outreach/meetup"target="blank">National meet-up</a>)</li>
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<li>A symposium and panel session was organized with to inform government officials about the risks and possibilities of synthetic biology, sparking the public debate about the use of synthetic biology (see <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/outreach/policy"target="blank">policy</a>)</li>
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<li>Interviewed and discussed our project with panama disease specialists and stakeholders (see <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/outreach/interviews"target="blank">interview</a>)</li>
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<li>Participated in <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/project/interlab"target="blank">interlab study</a></li>
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<li>Met all criteria for gold</li>
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</ul>
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<p><b>For more details check out our <a class="soft_link" href="https://2014.igem.org/Team:Wageningen_UR/overview/achievements"target="blank">achievements</a> page!</b></p>
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<h1>Sponsors</h1>
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<div class="sponsor_box">
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<a class="sponsor_rectangle" href="http://www.wageningenur.nl/en/Benefactors/Service/About-Wageningen-University-Fund.htm" target="blank"><img src="https://static.igem.org/mediawiki/2014/6/6f/Wageningen_UR_WUF_EN.png" style="width:150px"></a>
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<a class="sponsor_rectangle" href="http://www.erasynbio.eu/" target="blank"><img src="https://static.igem.org/mediawiki/2014/e/ea/Wageningen_UR_erasynbio.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.synenergene.eu/" target="blank"><img src="https://static.igem.org/mediawiki/2014/4/48/Wageningen_UR_synenergene.png"></a>
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<a class="sponsor_rectangle" href="http://www.snapgene.com/" target="blank"><img src="https://static.igem.org/mediawiki/2014/f/f0/Wageningen_UR_snapgene.png"></a>
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<a class="sponsor_rectangle" href="http://www.sigmaaldrich.com/sigma-aldrich/home.html" target="blank"><img src="https://static.igem.org/mediawiki/2014/6/61/Wageningen_UR_sigma.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.gilson.com/" target="blank"><img src="https://static.igem.org/mediawiki/2014/b/be/Wageningen_UR_gilson.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.neb.com/" target="blank"><img src="https://static.igem.org/mediawiki/2014/d/da/Wageningen_UR_neb.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.dsm.com/corporate/home.html" target="blank"><img src="https://static.igem.org/mediawiki/2014/9/9f/Wageningen_UR_dsm.png"></a>
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<a class="sponsor_wur" href="http://www.wageningenur.nl/en.htm" target="blank"><img src="https://static.igem.org/mediawiki/2014/6/6d/Wageningen_UR_sponsor_departements1.jpg"></a>
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<div class="clear"></div>
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<a class="sponsor_square" href="http://www.vlaggraduateschool.nl/" target="blank"><img src="https://static.igem.org/mediawiki/2014/8/85/Wageningen_UR_vlag.jpg"></a>
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<a class="sponsor_square" href="http://www.be-basic.org/" target="blank"><img src="https://static.igem.org/mediawiki/2014/c/c5/Wageningen_UR_be-basic.png"></a>
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                                <div class="clear"></div>
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<p>Protecting the natural biodiversity is a big part of our project. When the Fusarium threat has been detected and subsequently cleaned up, the bacteria will activate a kill-switch. In addition to this, spread of the genetically modified material will be prevented by a double dependent plasmid system in which loss or gain of one of the two modified plasmids will cause the host to kill itself.</p>
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Latest revision as of 02:50, 18 October 2014

Wageningen UR iGEM 2014

 

 

 

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visits


Saving the bananas

Every time you go to the supermarket or to the local market to buy bananas you take for granted that there will be bananas readily available for you to enjoy and savour. However, banana plants are under threat and a world without bananas is more plausible than we all might imagine. Banana plants are threatened by Fusarium oxysporum. This pathogenic soil fungus enters plants through their root systems and lives off of them until they die. This fungus currently threatens all banana plants worldwide. Bananas, apart from being a delicious fruit, have economic importance in many tropical regions and are an important part of the diet. These are the reasons why the iGEM team Wageningen 2014 has designed and created BananaGuard, a genetic system in Pseudomonas putida that detects the presence of F. oxysporum in the soil and produces a combination of antifungals to remove the fungal threat. If you want to read more about the system and our work during the last six months, the iGEM team Wageningen 2014 invites you to continue surfing around our Wiki.


Visualisation of BananaGuard. Watch this video on youtube.

Going for Gold

As an iGEM team we worked very hard for the past couple of months with the medal requirements in mind. Below is a list of why we deserve gold this year.


Bronze


  • Registered for iGEM
  • Completed judging form
  • Have a team Wiki
  • Present a poster and talk at the iGEM jamboree
  • Documented attributions
  • Documented and submitted 27 parts to the iGEM Registry
  • Met criteria for bronze

Silver



Gold


  • Improved the functions of existing BioBrick promoter parts (BBa_K1493504, BBa_K1493501) by an extensive characterization
  • Collaborated with iGEM TU_Delft-Leiden from the Netherlands by quantifying biofilm production formed in E. coli (see collaboration)
  • Organized the first Dutch National meetup with Dutch iGEM teams and laid a foundation for collaboration with the Dutch Institute of Health and Environment (RIVM) (see National meet-up)
  • A symposium and panel session was organized with to inform government officials about the risks and possibilities of synthetic biology, sparking the public debate about the use of synthetic biology (see policy)
  • Interviewed and discussed our project with panama disease specialists and stakeholders (see interview)
  • Participated in interlab study
  • Met all criteria for gold

For more details check out our achievements page!



Sponsors

Retrieved from "http://2014.igem.org/Team:Wageningen_UR"