"
Team:WPI-Worcester/Our-Construct
From 2014.igem.org
(Difference between revisions)
Cllajeunesse (Talk | contribs) |
Cllajeunesse (Talk | contribs) |
||
| Line 4: | Line 4: | ||
<meta name="generator" content="MediaWiki 1.16.5" /> | <meta name="generator" content="MediaWiki 1.16.5" /> | ||
<link rel="shortcut icon" href="/favicon.ico" /> | <link rel="shortcut icon" href="/favicon.ico" /> | ||
| - | <link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title=" | + | <link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2014.igem.org (en)" /> |
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:WPI-Worcester&action=creativecommons" rel="meta" /> | <link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:WPI-Worcester&action=creativecommons" rel="meta" /> | ||
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" /> | <link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" /> | ||
| - | <link rel="alternate" type="application/atom+xml" title=" | + | <link rel="alternate" type="application/atom+xml" title="2014.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&feed=atom" /> <title>Team:WPI-Worcester - 2014.igem.org</title> |
<style type="text/css" media="screen, projection">/*<![CDATA[*/ | <style type="text/css" media="screen, projection">/*<![CDATA[*/ | ||
@import "/wiki/skins/common/shared.css?270"; | @import "/wiki/skins/common/shared.css?270"; | ||
| Line 30: | Line 30: | ||
wgVariantArticlePath=false, | wgVariantArticlePath=false, | ||
wgActionPaths={}, | wgActionPaths={}, | ||
| - | wgServer="https:// | + | wgServer="https://2014.igem.org", |
wgCanonicalNamespace="", | wgCanonicalNamespace="", | ||
wgCanonicalSpecialPageName=false, | wgCanonicalSpecialPageName=false, | ||
| Line 51: | Line 51: | ||
wgDigitTransformTable=["", ""], | wgDigitTransformTable=["", ""], | ||
wgMainPageTitle="Main Page", | wgMainPageTitle="Main Page", | ||
| - | wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": " | + | wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2014.igem.org", "5": "2014.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"}, |
| - | wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, " | + | wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2014.igem.org": 4, "2014.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7}, |
| - | wgSiteName=" | + | wgSiteName="2014.igem.org", |
wgCategories=[], | wgCategories=[], | ||
| - | wgMWSuggestTemplate="https:// | + | wgMWSuggestTemplate="https://2014.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest", |
| - | wgDBname=" | + | wgDBname="2014_igem_org", |
wgSearchNamespaces=[0], | wgSearchNamespaces=[0], | ||
wgMWSuggestMessages=["with suggestions", "no suggestions"], | wgMWSuggestMessages=["with suggestions", "no suggestions"], | ||
| Line 91: | Line 91: | ||
<div id="search-controls" class="noprint"> | <div id="search-controls" class="noprint"> | ||
<form action="/Special:Search" id="searchform"> | <form action="/Special:Search" id="searchform"> | ||
| - | <input id="searchInput" name="search" type="text" title="Search | + | <input id="searchInput" name="search" type="text" title="Search 2014.igem.org [f]" accesskey="f" value="" /> |
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" /> | <input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" /> | ||
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /> | <input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /> | ||
| Line 101: | Line 101: | ||
<h1 class="firstHeading">Team:WPI-Worcester</h1> | <h1 class="firstHeading">Team:WPI-Worcester</h1> | ||
<div id="bodyContent"> | <div id="bodyContent"> | ||
| - | <h3 id="siteSub" class='noprint'>From | + | <h3 id="siteSub" class='noprint'>From 2014.igem.org</h3> |
<div id="contentSub"></div> | <div id="contentSub"></div> | ||
<!-- | <!-- | ||
| Line 600: | Line 600: | ||
<ul> | <ul> | ||
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Survey">Survey</a></li> | <li><a href="https://2014.igem.org/Team:WPI-Worcester/Survey">Survey</a></li> | ||
| - | <li><a href="https:// | + | <li><a href="https://2014.igem.org/Team:WPI-Worcester/Outreach">Outreach</a></li> |
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Collaborations">Collaborations</a></li> | <li><a href="https://2014.igem.org/Team:WPI-Worcester/Collaborations">Collaborations</a></li> | ||
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Interlab">Interlab Study</a></li> | <li><a href="https://2014.igem.org/Team:WPI-Worcester/Interlab">Interlab Study</a></li> | ||
| Line 622: | Line 622: | ||
</br> | </br> | ||
| - | |||
<h4>Our Construct</h4><p><center><img src="https://static.igem.org/mediawiki/2014/thumb/e/e0/WPI_OurConstruct.png/800px-WPI_OurConstruct.png"/></center></p><p>The construct developed in this project is a combination of the N-terminal domain of BclA and the CAEV p28 antigen. The sequence encoding the N-terminal domain of BclA is ligated to the sequence for the CAEV p28 antigen. This plasmid is then combined with a ribosome binding site, a constitutive promoter, and a double terminator in order to make a complete construct. The resulting plasmid allows the <i>E. coli</i> it is transformed into to express the CAEV p28 antigen connected to the N-terminal domain of BclA, which is anchored in the outer surface of the plasma membrane. This plasmid is transformed into competent <i>E. coli</i>, which then express CAEV on their membrane surface, thanks to the anchoring of BclA’s N-terminal domain. | <h4>Our Construct</h4><p><center><img src="https://static.igem.org/mediawiki/2014/thumb/e/e0/WPI_OurConstruct.png/800px-WPI_OurConstruct.png"/></center></p><p>The construct developed in this project is a combination of the N-terminal domain of BclA and the CAEV p28 antigen. The sequence encoding the N-terminal domain of BclA is ligated to the sequence for the CAEV p28 antigen. This plasmid is then combined with a ribosome binding site, a constitutive promoter, and a double terminator in order to make a complete construct. The resulting plasmid allows the <i>E. coli</i> it is transformed into to express the CAEV p28 antigen connected to the N-terminal domain of BclA, which is anchored in the outer surface of the plasma membrane. This plasmid is transformed into competent <i>E. coli</i>, which then express CAEV on their membrane surface, thanks to the anchoring of BclA’s N-terminal domain. | ||
</p> | </p> | ||
| - | |||
</br> | </br> | ||
</br> | </br> | ||
</br> | </br> | ||
</html> | </html> | ||
Revision as of 03:53, 16 October 2014
"
Team:WPI-Worcester
From 2014.igem.org
Our Construct
The construct developed in this project is a combination of the N-terminal domain of BclA and the CAEV p28 antigen. The sequence encoding the N-terminal domain of BclA is ligated to the sequence for the CAEV p28 antigen. This plasmid is then combined with a ribosome binding site, a constitutive promoter, and a double terminator in order to make a complete construct. The resulting plasmid allows the E. coli it is transformed into to express the CAEV p28 antigen connected to the N-terminal domain of BclA, which is anchored in the outer surface of the plasma membrane. This plasmid is transformed into competent E. coli, which then express CAEV on their membrane surface, thanks to the anchoring of BclA’s N-terminal domain.
