We were capable of synthesizing magnetic nanoparticles with diameters of 22 nanometers. After coating them with Silicon dioxide their diameter was about 39 nanometers, these sizes were determined by Dynamic Light Scattering, a technique that shows the size distribution of the particles. The aminosilinization could not be confirmed by infrared spectra because the small amount of the amino groups in the sample. However the protein could be immobilized in the particle, showing us that the particles were correctly amino functionalized.

Without a transmission electronic microscope it seemed a hard work to determine if the DNA and the protein had been attached to the nanoparticles, however we had another cheap yet powerful tool: Electrophoresis.
By comparing a the isolated plasmid and protein with a sample of drills in agarose gel we could see that neither the protein nor the plasmid would shift when they were immobilized in the nanoparticles. Even after adding restriction enzymes, the plasmid wouldn't shift in the electrophoresis gel.

Without a transmission electronic microscope it seemed a hard work to determine if the DNA and the protein had been attached to the nanoparticles, however we had another cheap yet powerful tool: Electrophoresis.
By comparing a the isolated plasmid and protein with a sample of drills in agarose gel we could see that neither the protein nor the plasmid would shift when they were immobilized in the nanoparticles. Even after adding restriction enzymes, the plasmid wont shift in the electrophoresis gel.