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Team:Valencia UPV/Project/results/trichome expression
From 2014.igem.org
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| - | <div align="center"><img width=" | + | <div align="center"><img width="60%" src="https://static.igem.org/mediawiki/2014/2/28/VUPVPCPS2-GFP.png" alt="GFP"></img></div><br/> |
<p>This construct was inserted into the plant, <a class="italic">Nicotiana benthamiana</a> (<a class="blue-bold">see agroinfiltration</a>) and after 5 days the expression of GFP was checked. A <a class="red-bold">LUPA LEICA</a> was used with an excitation wavelength of 395 nm.</p><br/><br/> | <p>This construct was inserted into the plant, <a class="italic">Nicotiana benthamiana</a> (<a class="blue-bold">see agroinfiltration</a>) and after 5 days the expression of GFP was checked. A <a class="red-bold">LUPA LEICA</a> was used with an excitation wavelength of 395 nm.</p><br/><br/> | ||
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| - | <div align="center"><img width=" | + | <div align="center"><img width="60%" src="https://static.igem.org/mediawiki/2014/2/24/VUPVTrichomesGFP.jpg" alt="trichome1"></img></div> |
| - | <div align="center"><img width=" | + | <div align="center"><img width="60%" src="https://static.igem.org/mediawiki/2014/5/57/VUPVNegativecontrolGFP.jpg" alt="trichome2"></img></div><br/> |
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Revision as of 23:18, 12 October 2014
This project main objective was to produce insect sexual pheromones in genetically engineered plants, but once this goal was achieved, another important question raised, would that plant be able to release these pheromones into the environment.
Therefore, mimicking nature, the chosen approach was to express the enzymes of the pheromone production pathway (see biosynthesis) specifically on glandular trichomes, which are plant organs, specialized on releasing volatiles. With this objective, a glandular trichome-specific promoter (PCPS2) was obtained from Nicotiana tabacum genome for further use. (see Module: Pheromone release)
First of all, the promoter needed to be tested. PCPS2 promoter was joined with GFP in order to look for localized fluorescence in the trichomes. (see GoldenBraid 2.0)
This construct was inserted into the plant, Nicotiana benthamiana (see agroinfiltration) and after 5 days the expression of GFP was checked. A LUPA LEICA was used with an excitation wavelength of 395 nm.
This is what was observed:
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