Team:Utah State/BioBricksAllParts

From 2014.igem.org

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<h2>BioBricks</h2>
<h2>BioBricks</h2>
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<h3>Favorite Parts</h3>
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<h2> All Parts </h2>
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<p>
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<h4><a href="http://parts.igem.org/Part:BBa_K1418002"> BBa_K1418002</a> </h4>
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</p>
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<p>This part was constructed as an inducible chlorophyllase enzyme generator with a C-terminal 10x-Histidine tag to allow for simple purification using nickel affinity chromatography. Refer to our <a href="https://2014.igem.org/Team:Utah_State/Project"> project page</a> for background information on chlorophyllase and to our <a href="https://2014.igem.org/Team:Utah_State/Results/Chlorophylasse"> chlorophyllase results page</a> to see how we produced, purified, and tested this enzyme.
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<h4>Intermediate parts (N- to C-terminal)</h4>
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K208010 – Composite part of R0010 (a lac inducible promoter) and B0034 (a ribosome binding site with 1.0 level of efficiency)
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K1418001 – BioBrick encoding a chlorophyllase enzyme (TaCHL) found in <i>Triticum aestivum</i>, a common species of wheat. RFC 23 compatible.
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K844000 – A 10x-Histidine tag used to purify chlorophyllase enzymes using nickel affinity chromatography. RFC 23 compatible.
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B0015 – Composite part of two transcriptional terminators.
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<div align="center">
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<img align="center" src="https://static.igem.org/mediawiki/2014/1/1b/2014USU_K1418002PlasmidLinear.png"  width="442" height="57" alt="USU 2014iGem2014;" />
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<p class="imageCaption">
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<strong> Figure 1. </strong> Diagram of part K1418002 plasmid map.
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<h4><a href="http://parts.igem.org/Part:BBa_K1418012"> BBa_K1418012</a></h4>
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<p>This part was constructed as an inducible cellulase enzyme generator with a C-terminal 10x-Histidine tag to allow for simple purification using nickel affinity chromatography. Refer to our <a href="https://2014.igem.org/Team:Utah_State/Project"> project page</a> for background information on chlorophyllase and to our <a href="https://2014.igem.org/Team:Utah_State/Results/Cullolase"> cellulase results page</a> to see how we produced, purified, and tested this enzyme.
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</p>
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<p>
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<h4>Intermediate parts (N- to C-terminal)</h4>
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</p>
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<p>
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K208010 – Composite part of R0010 (a lac inducible promoter) and B0034 (a ribosome binding site with 1.0 level of efficiency)
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</p>
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K1418011 – BioBrick twin of K118023 with double stop codons removed and designed as RFC 23 compatible.
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K844000 – A 10x-Histidine tag used to purify cellulase enzymes using nickel affinity chromatography. RFC 23 compatible.
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</p>
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<p>
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B0015 – Composite part of two transcriptional terminators.
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</p>
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<br>
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<div align="center">
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<img align="center" src="https://static.igem.org/mediawiki/2014/9/97/2014USU_K1418012PlasmidLinear.png"  width="449" height="57" alt="USU 2014iGem2014;" />
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<p class="imageCaption">
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<strong> Figure 2. </strong> Diagram of part K1418012 plasmid map.
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<h4><a href="http://parts.igem.org/Part:BBa_K1418061"> BBa_K1418061</a></h4>
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<p>This part was constructed to demonstrate the immobilization of green fluorescent protein (GFP) to bioplastic granules. Once this device was proven to function as expected, we were able to construct a similar genetic circuit with our stain-fighting enzymes in place of the GFP.
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<h4>Intermediate parts (N- to C-terminal)</h4>
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K934001 – phaCAB operon which encodes the 3 necessary enzymes in the bioplastic (PHB) pathway, preceded by native promoter. All from <i>Ralstonia eutropha</i> H16.
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K208010 – Composite part of R0010 (a lac inducible promoter) and B0034 (a ribosome binding site with 1.0 level of efficiency).
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K1418062 – Green fluorescent protein with double stop codons removed and XhoI site removed. RFC 23 compatible.
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K1418060 – BioBrick encoding PHB synthase enzyme (PhaC) with double stop codon following the end of the coding region. RFC 23 compatible.
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B0015 – Composite part of two transcriptional terminators.
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<br>
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<div align="center">
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<img align="center" src="https://static.igem.org/mediawiki/2014/a/a7/2014USU_K1418061PlasmidLinear.png"  width="467" height="60" alt="USU 2014iGem2014;" />
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<p class="imageCaption">
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<strong> Figure 3. </strong> Diagram of part K1418061 plasmid map.
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<groupparts>iGEM014 Utah_State</groupparts>
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:::::::::::::::::<groupparts>iGEM014 Utah_State</groupparts>
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Latest revision as of 01:18, 18 October 2014

Project
Home
Introduction
Design
Team Goals
Results
Chlorophyllase
Cellulase
Amylase
Immobolized Bioplastic
Medal Requirements
Team
BioBricks
Favorite Parts
All Parts
Policy & Practices/Outreach
Policy & Practices
Discover Biological Engineering
Engineering State
Interlab Study
Safety
About Our Lab
Safety Form
Protocols
Attributions

BioBricks

All Parts

<groupparts>iGEM014 Utah_State</groupparts>
Retrieved from "http://2014.igem.org/Team:Utah_State/BioBricksAllParts"