Revision as of 14:00, 11 October 2014

Home

Failed to load tracking. JS is probably not enabled

Background

Controlling the movement of our E.coli was essential for tracking down the pathogen. We chose to do so by controlling the expression of cheZ, a gene in the chemotactic pathway of E.coli. Without it, E.coli is immotile.

System design

A construct with an RBS, a promoter and cheZ was created and inserted into a cheZ-knockout strain to see if motility could be restored. In a later stage, cheZ was to be coupled with the system created by the Sensing group.

Result

We used a swarm plate assay to assess if motility had been restored. This was the case in one of many experiments. Positive controls indicated that the results of the swarm plate assay were not reproducible, however.

Parts

Assembly Plan

Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.

Back to Top

@@ Line 21: / Line 21: @@
 <h2 class="overview">System design</h2>
-<p class="box_text">A construct with an RBS, a promoter and <i>cheZ</i> was created and inserted into a <i>cheZ</i>-knockout strain to see if motility could be restored. Three promoters with different strengths were used to see how different expression levels of <i>cheZ</i> would affect motility. Finally, <i>cheZ</i> was to be coupled with the system created by the Sensing group.</p>
+<p class="box_text">A construct with an RBS, a promoter and <i>cheZ</i> was created and inserted into a <i>cheZ</i>-knockout strain to see if motility could be restored. In a later stage, <i>cheZ</i> was to be coupled with the system created by the Sensing group.</p>
 </a>
@@ Line 31: / Line 31: @@
 <h2 class="overview">Result</h2>
-<p class="box_text">By inoculating our <i>E.coli</i> on swarm plates, we were able to observe if their motility had been restored. The motility of cells containing the construct was only restored in one of many experiments. Positive controls on the swarm plate assay indicated that results were often not reproducible, however. </p>
+<p class="box_text">We used a swarm plate assay to assess if motility had been restored. This was the case in one of many experiments. Positive controls indicated that the results of the swarm plate assay were not reproducible, however.</p>
 </a>
 </div>

Team:Uppsala/Project Targeting

From 2014.igem.org