Team:UMayor-Chile/Project

From 2014.igem.org

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  </head>
 +
 
 +
  <body>
 +
    <!--navigation menu -->
 +
    <table width="100%">
 +
      <tbody>
 +
        <tr heigth="15px"></tr>
 +
        <tr heigth="75px">
 +
          <td style="border:1px solid black;" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://2014.igem.org/Team:UMayor-Chile" style="color:#000000">Home</a>
 +
          </td>
 +
          <td style="border:1px solid black;" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://2014.igem.org/Team:UMayor-Chile/Team" style="color:#000000">Our Polis</a>
 +
          </td>
 +
          <td style="border:1px solid black;" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://igem.org/Team.cgi?year=2014&amp;team_name=UMayor-Chile" style="color:#000000">Official Team Profile</a>
 +
          </td>
 +
          <td style="border:1px solid black" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://2014.igem.org/Team:UMayor-Chile/Project" style="color:#000000">E. coli Troyhorse</a>
 +
          </td>
 +
          <td style="border:1px solid black;" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://2014.igem.org/Team:UMayor-Chile/Parts" style="color:#000000">The DNA weapons of our Troyhorse</a>
 +
          </td>
 +
          <td style="border:1px solid black;" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://2014.igem.org/Team:UMayor-Chile/Notebook" style="color:#000000">Out the lab</a>
 +
          </td>
 +
          <td style="border:1px solid black;" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://2014.igem.org/Team:UMayor-Chile/Safety" style=" color:#000000">Safety</a>
 +
          </td>
 +
          <td style="border:1px solid black;" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://2014.igem.org/Team:UMayor-Chile/Attributions" style="color:#000000">Attributions</a>
 +
          </td>
 +
          <td align="center">
 +
            <a href="https://2014.igem.org/Main_Page">
 +
              <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px">
 +
            </a>
 +
          </td>
 +
        </tr>
 +
      </tbody>
 +
    </table>
 +
    <!--end navigation menu -->
 +
    <div class="gwd-div-t8w1"><span class="gwd-span-suxd">The cystic fibrosis</span>
 +
 +
      <div>
 +
        <br>
 +
      </div>
 +
      <div>
 +
        <br>
 +
      </div>
 +
    </div>
 +
    <div class="gwd-div-zi30">
 +
      <div class="gwd-div-5ynf"><span class="gwd-span-gks4">Our
 +
troyhorse warriors have a plan!!</span>
-
<!--main content -->
+
      </div>
-
<table width="70%" align="center">
+
    </div>
 +
    <div class="gwd-div-16g8">
 +
      <div><span class="gwd-span-bby2">Our troyhorse warriors have a plan!!</span>
 +
      </div>
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      <div><span class="gwd-span-13sl"><br></span>
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<!--welcome box -->
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      </div>
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<tr>
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      <div><span class="gwd-span-5rqd">We propose smart warriors E. coli for the battle against the P. aeruginosa. We are training them now, teaching the action plan!!! Pay attention...</span>
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<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<br>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:UMayor-Chile/Project&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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      </div>
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    <img src="https://static.igem.org/mediawiki/2014/a/a2/2cz8xw6.png" class="gwd-img-7ff3">
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    <div class="gwd-div-rsut">
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      <div><span class="gwd-span-8joy">http://redsalud.uc.cl/ucchristus/vidasaludable/glosario/F/fibrosis_quistica.act</span>
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<!--navigation menu -->
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      </div>
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<td align="center" colspan="3">
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      <div><span class="gwd-span-hz9p"><br></span>
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<table  width="100%">
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      </div>
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<tr heigth="15px"></tr>
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      <div><span class="gwd-span-sbih">http://www.fibrosisquisticadelpancreas.cl/noticias/10-generales/16-ministro-de-salud-inauguro-congreso-de-actualizacion-en-fibrosis-quistica-.html</span>
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<tr heigth="75px">  
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      </div>
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      <div><span class="gwd-span-1bo4"><br></span>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile"style="color:#000000">Home </a> </td>
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      <div><span class="gwd-span-a3q8">http://www.scielo.cl/scielo.php?script=sci_arttext&amp;pid=S0370-41062014000300003</span>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile/Team"style="color:#000000"> Team </a> </td>
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      <div><span class="gwd-span-07pf"><br></span>
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<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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      </div>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=UMayor-Chile"style="color:#000000"> Official Team Profile </a></td>
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      <div><span class="gwd-span-qet1">http://www.cff.org/</span>
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<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile/Project"style="color:#000000"> Project</a></td>
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      <div><span class="gwd-span-t5ry"><br></span>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile/Parts"style="color:#000000"> Parts</a></td>
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      <div><span class="gwd-span-dk6w">Taylor Sitarik Cohen &amp; Alice Prince. Cystic fibrosis: a mucosal immunodeficiency syndrome (doi:10.1038/nm.2715)</span>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile/Modeling"style="color:#000000"> Modeling</a></td>
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    </div>
 +
    <div class="gwd-div-k4ez"></div>
 +
    <div class="gwd-div-zoic">
 +
      <div><span class="gwd-span-djbn">Say goodbye everybody, the antibiotics are now available:</span>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile/Notebook"style="color:#000000"> Notebook</a></td>
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      <div><span class="gwd-span-yg95"><br></span>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile/Safety"style=" color:#000000"> Safety </a></td>
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      <div><span class="gwd-span-znew">&nbsp;There’s no protection of the biofilm. Both P. aeruginosa and our E. coli Troyhorse are exposed to the final cut… a massive antibiotic attack!!!</span>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile/Attributions"style="color:#000000"> Attributions </a></td>
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      <div>
 +
        <br>
 +
      </div>
 +
    </div>
 +
    <div class="gwd-div-hk3q">
 +
      <div><span class="gwd-span-pwtb">&nbsp;The first part of the plan is simple: looking for the Biofilm fortress.</span>
 +
      </div>
 +
      <div><span class="gwd-span-wk45"><br></span>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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      </div>
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</tr>
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      <div><span class="gwd-span-v3j0">&nbsp; &nbsp;We have to find our enemy. Our warriors will to enter to the fortress been guide by the clues left by our enemies, the Pseudomonas aeruguinosa.</span>
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</table>
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</tr>
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      </div>
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</tr>
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    </div>
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</td>
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    <div class="gwd-div-ienk">
 +
      <div><span class="gwd-span-pfyf">Well, here we are… it’s time to start the part II:</span>
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<tr> <td colspan="3"  height="15px"> </td></tr>
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      </div>
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<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
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      <div><span class="gwd-span-ap0f"><br></span>
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<tr> <td colspan="3"  height="5px"> </td></tr>
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 +
      </div>
 +
      <div><span class="gwd-span-37e7">&nbsp; Our E. coli tryhorse now know they are in the zone. It’s time to start the attack. Our enemy are protected by his thick barrier and our warriors know his weak point.&nbsp;</span>
 +
      </div>
 +
    </div>
 +
    <div class="gwd-div-orp2">
 +
      <div><span class="gwd-span-9fwt">They are under attack!!! Pseudomonas are communicating themselves for rebuild the biofilm:</span>
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<!--Project content  -->
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      </div>
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<tr><td > <h3> Project Description </h3></td>
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      <div><span class="gwd-span-zshs"><br></span>
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<td ></td >
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<td > <h3> Content</h3></td>
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</tr>
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<tr>
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      </div>
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<td width="45%"  valign="top">  
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      <div><span class="gwd-span-06vs">&nbsp; We have to cut the communications between the Pseudomonas as an intelligent strategy. The biofilm it’s a community work. If they can´t send signals, they won’t work in community.</span>
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<p>Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs) </p>
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<br>
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<h3>References </h3>
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<p>
+
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iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you. </p>
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</td>
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<td></td>
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      </div>
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<td  width="45%" valign="top">
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    </div>
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<p> You can use these subtopics to further explain your project</p>
+
    <div class="gwd-div-4inh">
 +
      <div class="gwd-div-86v0"><span class="gwd-span-v6y8">70,000 people with CF worldwide, a lifespan depending of the resources (in USA the lifespan it’s about 40 years old, while in Chile the average lifespan it’s about 18 years old)</span>
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<ol>
+
      </div>
-
<li>Overall project summary</li>
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    </div>
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<li>Project Details</li>
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    <div class="gwd-div-doj2">
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<li>Materials and Methods</li>
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      <div class="gwd-div-ay25"><span class="gwd-span-bwuo">Scene from Patient Assistance Resource Center video (Youtube). Cystic Fibrosis Foundation.</span>
-
<li>The Experiments</li>
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-
<li>Results</li>
+
-
<li>Data analysis</li>
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<li>Conclusions</li>
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</ol>
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<p>
+
      </div>
-
It's important for teams to describe all the creativity that goes into an iGEM project, along with all the great ideas your team will come up with over the course of your work.  
+
    </div>
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</p>
+
    <div class="gwd-div-gnb3">
 +
      <div><span class="gwd-span-5all">It’s a disease caused by the expression of a deficient transmembrane protein called CFTR. This affects different kind of cells, mainly the epithelial cells from the airway and pancreas, causing the production of a defective mucus.</span>
-
<p>
+
      </div>
-
It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted.
+
      <div><span class="gwd-span-bnjd"><br></span>
-
</p>
+
-
</td>
+
      </div>
 +
      <div><span class="gwd-span-gxwd">&nbsp;In the lungs, this defective mucus leads that several opportunistic pathogens enter to the lower airway and initiate an infection, with a consecutively progressive lung dysfunction.</span>
-
</tr>
+
      </div>
 +
    </div>
 +
    <img src="https://static.igem.org/mediawiki/2014/e/e9/Imagen1eng.png" class="gwd-img-4tve">
 +
    <img src="https://static.igem.org/mediawiki/2014/8/87/Cap1212.jpg" class="gwd-img-ezf3">
 +
    <div class="gwd-div-txah">
 +
      <p class="MsoNormal"><span lang="EN-US" class="gwd-span-0jrz"><span class="gwd-span-1gxd">One of the
 +
main pathogens found in an infection of the lungs are the <em>Pseudomonas
 +
aeruginosa</em>. When it colonize the airway, initiates the production of biofilm, a
 +
barrier that protect it of a lot of antimicrobial substances and gave best environment
 +
for it develop.</span>
 +
        <o:p></o:p>
 +
        </span>
 +
      </p>
 +
      <p class="MsoNormal"><span lang="EN-US"><span class="gwd-span-6n79"><span class="gwd-span-wzif">&nbsp;The actual treatments for controlling the symptoms
 +
consist in maintain controlled the pathogens, clear the airway of the sticky
 +
mucus, administrate complementary enzymes and regulate the function of the CFTR
 +
channel.&nbsp;</span></span>
 +
        <o:p></o:p>
 +
        </span>
 +
      </p>
 +
    </div>
 +
    <img src="https://static.igem.org/mediawiki/2014/8/8d/Construct.png" class="gwd-img-llex">
 +
  </body>
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</table>
 
</html>
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Latest revision as of 02:19, 18 October 2014


Project

Home Our Polis Official Team Profile E. coli Troyhorse The DNA weapons of our Troyhorse Out the lab Safety Attributions
The cystic fibrosis


Our troyhorse warriors have a plan!!
Our troyhorse warriors have a plan!!

We propose smart warriors E. coli for the battle against the P. aeruginosa. We are training them now, teaching the action plan!!! Pay attention...
http://redsalud.uc.cl/ucchristus/vidasaludable/glosario/F/fibrosis_quistica.act

http://www.fibrosisquisticadelpancreas.cl/noticias/10-generales/16-ministro-de-salud-inauguro-congreso-de-actualizacion-en-fibrosis-quistica-.html

http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0370-41062014000300003

http://www.cff.org/

Taylor Sitarik Cohen & Alice Prince. Cystic fibrosis: a mucosal immunodeficiency syndrome (doi:10.1038/nm.2715)
Say goodbye everybody, the antibiotics are now available:

 There’s no protection of the biofilm. Both P. aeruginosa and our E. coli Troyhorse are exposed to the final cut… a massive antibiotic attack!!!

 The first part of the plan is simple: looking for the Biofilm fortress.

   We have to find our enemy. Our warriors will to enter to the fortress been guide by the clues left by our enemies, the Pseudomonas aeruguinosa.
Well, here we are… it’s time to start the part II:

  Our E. coli tryhorse now know they are in the zone. It’s time to start the attack. Our enemy are protected by his thick barrier and our warriors know his weak point. 
They are under attack!!! Pseudomonas are communicating themselves for rebuild the biofilm:

  We have to cut the communications between the Pseudomonas as an intelligent strategy. The biofilm it’s a community work. If they can´t send signals, they won’t work in community.
70,000 people with CF worldwide, a lifespan depending of the resources (in USA the lifespan it’s about 40 years old, while in Chile the average lifespan it’s about 18 years old)
Scene from Patient Assistance Resource Center video (Youtube). Cystic Fibrosis Foundation.
It’s a disease caused by the expression of a deficient transmembrane protein called CFTR. This affects different kind of cells, mainly the epithelial cells from the airway and pancreas, causing the production of a defective mucus.

 In the lungs, this defective mucus leads that several opportunistic pathogens enter to the lower airway and initiate an infection, with a consecutively progressive lung dysfunction.

One of the main pathogens found in an infection of the lungs are the Pseudomonas aeruginosa. When it colonize the airway, initiates the production of biofilm, a barrier that protect it of a lot of antimicrobial substances and gave best environment for it develop.

 The actual treatments for controlling the symptoms consist in maintain controlled the pathogens, clear the airway of the sticky mucus, administrate complementary enzymes and regulate the function of the CFTR channel. 

Retrieved from "http://2014.igem.org/Team:UMayor-Chile/Project"