Team:UMayor-Chile/Project

From 2014.igem.org

(Difference between revisions)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
 
-
 
-
 
{{CSS/Main}}
{{CSS/Main}}
 +
{{team.umayor/removeStyles}}
<html>
<html>
<body style="background:#7EA99A">
<body style="background:#7EA99A">
 +
<div align="center"><a href="https://2014.igem.org/Team:UMayor-Chile" target="_blank"><img src="http://i57.tinypic.com/2e5uypz.png" border="0" alt="Image and video hosting by TinyPic"></a>
 +
<!--main content -->
<!--main content -->
-
<table width="70%" align="center">
+
<table width="80%" align="center">
Line 14: Line 14:
<tr>
<tr>
<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#02464F>
<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#02464F>
-
<h1 >WELCOME TO iGEM 2014! </h1>
+
<marquee>  <h1> <cente>  PROJECT IGEM TROY HORSE - UNIVERSIDAD MAYOR </center> </h1>               </marquee> <span style="color:#FFFFFF"> CHILE
-
<p>Your team has been approved and you are ready to start the iGEM season!
+
<p>Hello world!! We are an official team for the iGEM 2014. We are working so hard on our project TroyHorse, but we're having a really good experience
-
<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
+
<br>
<br>
-
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:UMayor-Chile/Project&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
+
<p style="color:#yellow"> <a href="https://2014.igem.org/wiki/index.php?title=Team:UMayor-Chile&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
</td>
</td>
</tr>
</tr>
Line 41: Line 40:
<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
-
<a href="https://igem.org/Team.cgi?year=2014&team_name=UMayor-Chile"style="color:#000000"> Official Team Profile </a></td>
+
<a href="https://igem.org/Team.cgi?year=2014&team_name=UMayor-Chile"style="color:#000000"> News and events </a></td>
<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>   
<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>   
Line 66: Line 65:
</table>
</table>
 +
<!--end navigation menu -->
</tr>
</tr>
-
</tr>
+
 
 +
 
</td>
</td>
<tr> <td colspan="3"  height="15px"> </td></tr>
<tr> <td colspan="3"  height="15px"> </td></tr>
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
+
<tr><td bgColor="#02464F" colspan="3" height="1px"> </tr>
<tr> <td colspan="3"  height="5px"> </td></tr>
<tr> <td colspan="3"  height="5px"> </td></tr>
 +
<!--requirements section -->
 +
<tr><td colspan="3"> <h3></h3></td></tr>
 +
<tr>
 +
<td width="45%"  valign="top">
-
<!--Project content -->
+
<!--  Block of content 4 -->
-
<tr><td > <h3> Cystic fibrosis (CF) </h3></td>
+
<table width="100%"  cellspacing="0" height="500px">
-
<td > </td >
+
<tr>
-
<td > <h3> Our troyhorse warriors have a plan!!!</h3></td>
+
<td  bgColor="#02464F"></td>
-
</tr>
+
<td width="975px" align="center" bgColor="#02464F" >
 +
<table  width="975px"  cellspacing="0" height="500px">
<tr>
<tr>
-
<td width="45%"  valign="top">  
+
<td width="95%" bgColor="#02464F"  rowspan="3" align="center">  
-
<p>70,000 people with CF worldwide, a lifespan depending of the resources (in USA the lifespan it’s about 40 years old, while in Chile the average lifespan it’s about 18 years old).</p>
+
<!-- Content goes here -->
-
<br>
+
 
-
<p>It’s a disease caused by the expression of a deficient transmembrane protein called CFTR. This affects different kind of cells, mainly the epithelial cells from the airway and pancreas, causing the production of a defective mucus.</p>
+
<h3 style="color:#ffffff;">The Cystic Fibrosis (CF)</h3>
-
<br>
+
<p>It’s a mutation in the glycoprotein CFTR (that have a rol as a chloride channel), which affects the entire system, including the immune system. When a patient is diagnosticated with Pseudomonas aeruginosa in his lungs, it is too difficult to eradicate it. One reason of this it’s the biofilm generated by P. aerugunosa, which protects this opportunistic pathogen from any attack, including the treatments with antibiotics. Our project aims to generate a bacteria capable of weakening the biofilm breaking one of the main components of this protection: the arginate; and shutting down the communication between the Pseudomonas colonies. The E. coli is capable to use the biofilms, making it a good candidate for use as a Trojan horse. We going to test the above mentioned functions in this chassis, using in vitro biofilms.</p>
-
<p> In the lungs, this defective mucus leads that several opportunistic pathogens enter to the lower airway and initiate an infection, with a consecutively progressive lung dysfunction.</p>
+
 
-
<br>
+
</td>
-
<p>One of the main pathogens found in an infection of the lungs are the Pseudomonas aeruginosa. When it colonize the airway, initiates the production of biofilm, a barrier that protect it of a lot of antimicrobial substances and gave best environment for it develop.</p>
+
 
-
<br>
+
</table>
-
<p>The actual treatments for controlling the symptoms consist in maintain controlled the pathogens, clear the airway of the sticky mucus, administrate complementary enzymes and regulate the function of the CFTR channel.</p>
+
-
<br>
+
-
<h3>References </h3>
+
-
<p>http://redsalud.uc.cl/ucchristus/vidasaludable/glosario/F/fibrosis_quistica.act</p>
+
-
<p>http://www.fibrosisquisticadelpancreas.cl/noticias/10-generales/16-ministro-de-salud-inauguro-congreso-de-actualizacion-en-fibrosis-quistica-.html</p>
+
-
<p>http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0370-41062014000300003</p>
+
-
<p>http://www.cff.org/</p>
+
-
<p>Taylor Sitarik Cohen & Alice Prince. Cystic fibrosis: a mucosal immunodeficiency syndrome (doi:10.1038/nm.2715)</p>
+
</td>
</td>
 +
<td bgColor="#02464F"></td>
 +
</tr>
 +
</table>
 +
<!-- end of Block-->
-
<td></td>
 
-
<td  width="45%"  valign="top">
 
-
<p> We propose smart warriors E. coli for the battle against the P. aeruginosa. We are training them now, teaching the action plan!!! Pay attention:</p>
 
-
<br>
 
-
<p>
+
<!-- Final block of content -->
-
1. The first part of the plan is simple: looking for the Biofilm fortress.</p>
+
<table  id="firstblock" width="100%"  cellspacing="0" height="15px">
 +
<tr>
 +
<td  bgColor="#FFFFFF"></td>
 +
<td width="975px" align="center" bgColor="#FFFFFF" >
-
<p>We have to find our enemy. Our warriors will enter to the fortress been guide by the clues left by our enemies, the Pseudomonas aeruguinosa.</p>
 
-
<br>
 
-
<p>2. Well, here we are… it’s time to start the part II:</p>
+
</td>
 +
<td bgColor="#FFFFFF"></td>
 +
</tr>
 +
</table>
 +
<!-- end of final block -->
-
<p>Our E. coli Troyhorse now know they are in the zone. It’s time to start the attack. Our enemy are protected by his thick barrier and our warriors know his weak point.</p>
+
<!--  Block of content 4 -->
-
<br>
+
<table width="100%"  cellspacing="0" height="500px">
 +
<tr>
 +
<td  bgColor="#02464F"></td>
 +
<td width="975px" align="center" bgColor="#02464F" >
-
<p>3. They are under attack!!! Pseudomonas are communicating themselves to rebuild the biofilm:</p>
+
<table  width="975px"  cellspacing="0" height="500px">
 +
<tr>
 +
<td width="95%" bgColor="#02464F"  rowspan="3" align="center">
 +
<!-- Content goes here -->
-
<p>
+
<h3 style="color:#ffffff;">Fig 1: Construct that allows E. coli to weak Pseudomonas's biofilm.</h3>
-
We have to cut the communications between the Pseudomonas as an intelligent strategy. The biofilm it’s a community work. If they can´t send signals, they won’t work in community.</p>
+
<p><a href="http://es.tinypic.com?ref=2d960zk" target="_blank"><img src="http://i60.tinypic.com/2d960zk.jpg" border="0" alt="Image and video hosting by TinyPic"></a></p>
-
<br>
+
-
<p>4. Say goodbye everybody, the antibiotics are now available:</p>
+
</td>
-
<p>
+
</td>
-
There’s no protection of the biofilm. Both P. aeruginosa and our E. coli Troyhorse are exposed to the final cut… a massive antibiotic attack!!!</p>
+
<td bgColor="#02464F"></td>
-
<br>
+
</tr>
 +
</table>
 +
<!-- end of Block-->
 +
 
 +
 
 +
 
 +
<!-- Final block of content -->
 +
<table  id="firstblock" width="100%"  cellspacing="0" height="2px">
 +
<tr>
 +
<td  bgColor="#FFFFFF"></td>
 +
<td width="975px" align="center" bgColor="#FFFFFF" >
</td>
</td>
 +
<td bgColor="#FFFFFF"></td>
 +
</tr>
 +
</table>
 +
<!-- end of final block -->
 +
<footer>
 +
 +
 +
<!--  Block of content 4 -->
 +
<table width="100%"  cellspacing="0" height="500px">
 +
<tr>
 +
<td  bgColor="#02464F"></td>
 +
<td width="975px" align="center" bgColor="#02464F" >
 +
 +
<table  width="975px"  cellspacing="0" height="500px">
 +
<tr>
 +
<td width="95%" bgColor="#02464F"  rowspan="3" align="center">
 +
<!-- Content goes here -->
 +
 +
<h3 style="color:#ffffff;">Fig 2: Construct that allows E. coli to weak Pseudomonas's biofilm.</h3>
 +
<p><a href="http://es.tinypic.com?ref=2cz8xw6" target="_blank"><img src="http://i58.tinypic.com/2cz8xw6.jpg" border="0" alt="Image and video hosting by TinyPic"></a></p>
 +
 +
</td> 
 +
 +
</td>
 +
<td bgColor="#02464F"></td>
</tr>
</tr>
 +
</table>
 +
<!-- end of Block-->
 +
 +
<!-- Final block of content -->
 +
<table  id="firstblock" width="100%"  cellspacing="0" height="2px">
 +
<tr>
 +
<td  bgColor="#FFFFFF"></td>
 +
<td width="975px" align="center" bgColor="#FFFFFF" >
 +
 +
</td>
 +
<td bgColor="#FFFFFF"></td>
 +
</tr>
</table>
</table>
 +
<!-- end of final block -->
 +
 +
<footer>
 +
 +
</footer>
</html>
</html>

Revision as of 01:12, 18 October 2014


Image and video hosting by TinyPic

PROJECT IGEM TROY HORSE - UNIVERSIDAD MAYOR

CHILE

Hello world!! We are an official team for the iGEM 2014. We are working so hard on our project TroyHorse, but we're having a really good experience

Click here to edit this page!

Home Team News and events Project Parts Modeling Out the lab Safety Attributions

The Cystic Fibrosis (CF)

It’s a mutation in the glycoprotein CFTR (that have a rol as a chloride channel), which affects the entire system, including the immune system. When a patient is diagnosticated with Pseudomonas aeruginosa in his lungs, it is too difficult to eradicate it. One reason of this it’s the biofilm generated by P. aerugunosa, which protects this opportunistic pathogen from any attack, including the treatments with antibiotics. Our project aims to generate a bacteria capable of weakening the biofilm breaking one of the main components of this protection: the arginate; and shutting down the communication between the Pseudomonas colonies. The E. coli is capable to use the biofilms, making it a good candidate for use as a Trojan horse. We going to test the above mentioned functions in this chassis, using in vitro biofilms.

Fig 1: Construct that allows E. coli to weak Pseudomonas's biofilm.

Image and video hosting by TinyPic