Team:ULB-Brussels/Safety
From 2014.igem.org
m |
m |
||
Line 26: | Line 26: | ||
<section style="text-align: justify; margin: 20px"></section> | <section style="text-align: justify; margin: 20px"></section> | ||
- | <p>However, in the current state of our project, plasmids are maintained in the bacterial population through the usual system of antibiotic resistance, since the toxin and the antitoxin are to be placed on different plasmids bearing different resistance genes. The properties of the TA systems are used to boost protein production but also for plasmid stability. In the final version of our project, the toxin gene will be inserted in the genomic DNA of the bacteria to reduce loss chances. Bacteria will be compelled to keep the plasmid bearing the genes of the antitoxin and the protein of interest, without the need of infamous antibiotic resistance genes</p> | + | <p>However, in the current state of our project, plasmids are maintained in the bacterial population through the usual system of antibiotic resistance, since the toxin and the antitoxin are to be placed on different plasmids bearing different resistance genes. The properties of the TA systems are used to boost protein production but also for plasmid stability. In the final version of our project, the toxin gene will be inserted in the genomic DNA of the bacteria to reduce loss chances. Bacteria will be compelled to keep the plasmid bearing the genes of the antitoxin and the protein of interest, without the need of infamous antibiotic resistance genes. </p> |
<p>If for one reason or another, the overproduction of a protein didn't result in a competitive disadvantage, antitoxin production can be put under the control of an inducible promoter, acting as a built-in biocontainment device. Indeed, if we chose an artificial inducer for the expression of the antitoxin (that is, an inducer not found in the nature), an escaped bacterium couldn't inhibit the activity of the toxin and woud quickly die outside of the bioreactor.</p> | <p>If for one reason or another, the overproduction of a protein didn't result in a competitive disadvantage, antitoxin production can be put under the control of an inducible promoter, acting as a built-in biocontainment device. Indeed, if we chose an artificial inducer for the expression of the antitoxin (that is, an inducer not found in the nature), an escaped bacterium couldn't inhibit the activity of the toxin and woud quickly die outside of the bioreactor.</p> | ||
Line 50: | Line 50: | ||
- Courses about security are applied for the workers and regular updated are organized <br> | - Courses about security are applied for the workers and regular updated are organized <br> | ||
- Pets are forbidden in the lab <br> | - Pets are forbidden in the lab <br> | ||
- | - Waste and contaminated materials are inactivated by an appropriate and validated method (by incineration or autoclave) </p><br> | + | - Waste and contaminated materials are inactivated by an appropriate and validated method (by incineration or autoclave). </p><br> |
<p>In conclusion, our system seems to be extremely safe and predictable. Its biggest danger consists of being used to produce a dangerous protein. This latter problem should be addressed on a case-by-case basis, by other institution than ours. Of course, our topical lab system is really safe, since it should only overproduce fluorescent reporters (GFP or RFP). However, Mighty Coli will be more useful to produce cumbersome proteins than usual fluorescent proteins.</p> | <p>In conclusion, our system seems to be extremely safe and predictable. Its biggest danger consists of being used to produce a dangerous protein. This latter problem should be addressed on a case-by-case basis, by other institution than ours. Of course, our topical lab system is really safe, since it should only overproduce fluorescent reporters (GFP or RFP). However, Mighty Coli will be more useful to produce cumbersome proteins than usual fluorescent proteins.</p> |
Revision as of 22:49, 11 October 2014
$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}} \newcommand{\Stabi}{\small Stabi}$ $\newcommand{\EColi}{\small E.coli} \newcommand{\SCere}{\small S.cerevisae}\\[0cm] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\PI}{\small PI}$ $\newcommand{\Igo}{\Large\mathcal{I}} \newcommand{\Tgo}{\Large\mathcal{T}} \newcommand{\Ogo}{\Large\mathcal{O}} ~$
|
---|