09/16/2014

Hello!!! Today we used the spectrofluorometer for the first time!!! We used DH5α without genetic modifications as “blank” and DH5α with p26G as positive control, grown in liquid medium LB for 16h.

We used:

Equipment: Hidex Chameleon.

Excitation filter: 340nm

Emission filter: 500nm

For creation of a GFP insanity expression levels curve, we diluted the sample of DH5α with p26G in liquid medium LB for 10^-1, 10^-2, 10^-3. So we’ll be able to compare and calculate GFP expression intensity, our blank should also be diluted. The intensity levels of fluorescence showed by the Hidex Chameleon are: