Team:UCLA/Notebook/Protocols

From 2014.igem.org

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<tr><td><br><b>Equipment for the collection of recombinant silk protocols</b></br><a href="https://static.igem.org/mediawiki/2014/a/ab/Equipment_Protocol.pdf">Equipment Protocol</a></td></tr>
<tr><td><br> <b>PCR for MASP1 and MASP2 </b></br>
<tr><td><br> <b>PCR for MASP1 and MASP2 </b></br>
<a href="https://static.igem.org/mediawiki/2014/archive/4/47/20140701232004%21IGEM_PCR_reaction_design.pdf">PCR Protocol</a></td></tr>
<a href="https://static.igem.org/mediawiki/2014/archive/4/47/20140701232004%21IGEM_PCR_reaction_design.pdf">PCR Protocol</a></td></tr>
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<tr><td><br><b>Silk Fibroin Extraction</b></br><a href="https://static.igem.org/mediawiki/2014/a/a7/Silk_Fibroin_Extraction_%26_Film_Formation_Protocol%281%29.pdf">Silk Fibroin Extraction Protocol</a></td></tr>
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<tr><td><br> <b>Silk Films</b></br>
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<a href="https://static.igem.org/mediawiki/2014/a/a9/Silk_Fibroin_Extraction_%26_Film_Formation_Protocol%282%29.pdf">Silk Films Protocol</a></td></tr>
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<tr><td><br> <b>Silk Films</b></br>
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<a href="https://static.igem.org/mediawiki/2014/d/d2/Silk_Fibroin_Extraction_%26_Film_Formation_Protocol%283%29.pdf">Electrospun fibers</a></td></tr>
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Revision as of 01:58, 2 July 2014

iGEM UCLA




Protocols

You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.


Equipment for the collection of recombinant silk protocols
Equipment Protocol

PCR for MASP1 and MASP2
PCR Protocol

Silk Fibroin Extraction
Silk Fibroin Extraction Protocol

Silk Films
Silk Films Protocol

Silk Films
Electrospun fibers


Reference

Materials Fabrication
Rockwood, Danielle N., Rucsanda C. Preda, Tuna Yücel, Xiaoqin Wang, Michael L. Lovett, and David L. Kaplan. "Materials Fabrication from Bombyx Mori Silk Fibroin." Nature Protocols 6.10 (2011): 1612-631. Web.


Production of MASP1/2
Teulé, Florence, Alyssa R. Cooper, William A. Furin, Daniela Bittencourt, Elibio L. Rech, Amanda Brooks, and Randolph V. Lewis. "A Protocol for the Production of Recombinant Spider Silk-like Proteins for Artificial Fiber Spinning." Nature Protocols 4.3 (2009): 341-55. Web.