Team:UB Indonesia
From 2014.igem.org
Project Description | |
UB IGEM TEAM is on progress in developing C3P (Cervical Cancer Care Program) as the theme to give solutions for cervical cancer cases. Starting from creating a rapid test tool using HPV DNA which is integrated with the software in smartphone, creating a high antioxidant tea for preventing efforts, and creating a kit for selecting potential anti-cancer herbs. | |
As we know, until now there has not been found a practical method to early detect cervical cancer. Making rapid test tool using HPV DNA become a great solution to suppress the spread of cervical cancer. On the other hand, the use of high antioxidant tea is an efficient way to prevent cervical cancer. While the herbal screening kit will facilitate the selection of potential anti-cancer Indonesia herbs. From some of these activities, is expected to acquire the technology or product whose function is to detect, prevent and provide raw material cervical cancer medicine. | |
According to Bosch FX (2003), cervical cancer is one of the most common cancers in women worldwide, with an estimated global incidence of 470,000 new cases and approximately 233,000 deaths per year. Then, we try to make an idea to build a new prototype for early detection to cervical cancer, especially caused by HPV 18 and HPV 16. From the previous results of the two teams, Calgary Team 2013 and Freiburg Team 2012, we build a new prototype with some combination from those teams. We learn about the prototype sensor from Calgary Team 2012 that consist of detector, linker, and reporter that combined into one small test pack. Then, to make the detector, we learn from Freiburg Team 2012 to constructing a new specific TALEs that can bind to our target sequences and we called it TALE 1 and TALE 2.
TALEs or Transcription Activator-Like Effectors comes from natural bacterial effector proteins used by Xanthomonas sp. to modulate gene transcription in host plants to facilitate bacterial colonization (Boch J and Bogdanove, 2010).The central region of the protein contains tandem repeats of 34 amino acids sequences (termed monomers) that are required for DNA recognition and binding (Kay, 2009). Here is the natural structure of TALE from Xanthomonas sp. :