Team:Toulouse/Safety

From 2014.igem.org

(Difference between revisions)
(Prototype team page)
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<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
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<!--SAFETY-->
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{{CSS/Main}}
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<html>
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<head>
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<!-- this section changes the default wiki template to a white full width background -->
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<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
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<h1 >WELCOME TO iGEM 2014! </h1>
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padding:3px;
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<p>Your team has been approved and you are ready to start the iGEM season!
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text-decoration: underline;
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<br>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Toulouse/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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body, #heading, #innercontent, #innercontenthome {
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<a href="https://2014.igem.org/Team:Toulouse"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:Toulouse/Team"style="color:#000000"> Team </a> </td>
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/*background Header*/
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Toulouse"style="color:#000000"> Official Team Profile </a></td>
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/* Barre de Menu*/
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<a href="https://2014.igem.org/Team:Toulouse/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:Toulouse/Parts"style="color:#000000"> Parts</a></td>
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    background-image:url(https://static.igem.org/mediawiki/2014/0/0e/Bt_home_1.png);
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<a href="https://2014.igem.org/Team:Toulouse/Modeling"style="color:#000000"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:Toulouse/Notebook"style="color:#000000"> Notebook</a></td>
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<a href="https://2014.igem.org/Team:Toulouse/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:Toulouse/Attributions"style="color:#000000"> Attributions </a></td>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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    background-image:url(https://static.igem.org/mediawiki/2014/d/d3/Bt_notebook_1.png);
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<!--safety content-->
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<tr><td > <h3> Welcome! </h3></td>
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<td ></td >
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<td > <h3> Timeline</h3></td>
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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    background-image:url(https://static.igem.org/mediawiki/2014/6/6a/Bt_attributions_1.png);
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    background-position:center top;
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    background-size: 100px 100px;
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    background-repeat : no-repeat;   
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 +
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    background-size: 100px 100px;
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    background-repeat : no-repeat;
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}
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<br>
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/*deroulement du menu*/
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<h3> Edit this page!</h3>
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#Menu {
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<p>
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    position : absolute;
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Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
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    width : 100%;
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<ul>
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    min-width :780px;
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<li> <a href=" ">Learn about lab Safety for Today</a></li>  
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    max-width : 1400px;
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<li> <a href="">Learn about Safety for the future of your project.</a></li>  
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 +
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 +
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 +
 
 +
<!-- STYLESHEET -->
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<!-- *********** --
 +
 
 +
    <link rel="stylesheet" href="https://2013.igem.org/Team:INSA_Toulouse/template/stylesheet?action=raw&amp;ctype=text/css" type="text/css" />
 +
 
 +
 
 +
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 +
 
 +
</head>
-->
-->
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</p>
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<body >
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<!--main content -->
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<table width="100%" align="center">
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<h3> Your Lab </h3>
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<!--navigation menu -->
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<div style="background-color : transparent" >
 +
<img id="bgHeader" src="https://static.igem.org/mediawiki/2014/f/f2/Branch_col.png" alt="header background" />
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</div>
 +
<div id="Menu" style="background-color:transparent;">
 +
    <div class="Item" style="left :5%;"> 
 +
        <a class="btHeaderA1" href="https://2014.igem.org/Team:Toulouse">Home</a>
 +
        </a>
 +
    </div>
 +
   
 +
    <div class="Item" style="left :15%">
 +
        <a class="btHeaderA2" href="https://2014.igem.org/Team:Toulouse/Team">Team</a>
 +
        <ul class="Item sub-nav" style="top : 125px;">
 +
            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Team" class="leaf">Our Team</a></li>
 +
            <li class="sub-nav-item" align="left"><a href="https://igem.org/Team.cgi?year=2014&team_name=Toulouse" class="leaf"> Official Team Profile </a></li>
 +
            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Team/Contact" class="leaf">Contact</a></li>
 +
            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Team/Gallery" class="leaf">Gallery</a></li>
 +
        </ul>
 +
    </div>
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>  
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    <div class="Item" style=" left :25%">
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<!--
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        <a class="btHeaderA3" href="https://2014.igem.org/Team:Toulouse/Project/Overview">Project</a>
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<gallery>
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        <ul class="Item sub-nav" style="top : 155px;">
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Image:Example2_Lab_1.png|The building our lab is in!
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            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Project/Overview" class="leaf">Overview</a></li>
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Image:Example2_Lab_2.png|The inside of our lab!
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            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Project/Fixation" class="leaf">Fixation</a></li>
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Image:Example2_Lab_3.png|Team Member 3 doing an experiment
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            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Project/Chemotaxis" class="leaf">Chemotaxis</a></li>
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Image:Example2_Lab_4.png|Working in biosafety cabinets
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            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Project/Fungicides" class="leaf">Fungicides</a></li>
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Image:Example2_Lab_5.png|Team all gloved up and ready for work!
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            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Project/Spreading" class="leaf">Spreading</a></li>
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Image:Example2_Lab_6.png|Equipment that we use to do SCIENCE!
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            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Modeling" class="leaf">Modeling</a></li>
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Image:Example2_Lab_7.png|We decorated this part of our lab
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            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Parts" class="leaf">Parts</a></li>
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Image:Example2_Lab_8.png|Whatever else you want
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        </ul>
-
</gallery>-->
+
    </div>
 +
   
 +
    <div class="Item" style=" left :35%"> 
 +
        <a class="btHeaderA4" href="https://2014.igem.org/Team:Toulouse/Safety">Human </br> Practice</a>
 +
        <ul class="Item sub-nav" style="top : 149px;">
 +
            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Safety" class="leaf">Safety</a></li>
 +
            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Human_Practice/Ethique" class="leaf">Ethique</a></li>
 +
            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Human_Practice/Meeting" class="leaf">Meeting</a></li>
 +
        </ul>
 +
    </div>
 +
    <div class="Item" style=" left :45%" > 
 +
        <a class="btHeaderA5" href="https://2014.igem.org/Team:Toulouse/Notebook/Calendar">Notebook</a>
 +
        <ul class="Item sub-nav" style="top : 160px;">
 +
            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Notebook/Calendar" class="leaf">Calendar</a></li>
 +
            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Notebook/Protocols" class="leaf">Protocols</a></li>
 +
        </ul>
 +
    </div>
-
</td>
+
    <div class="Item" style=" left : 55%">
-
<td></td>
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        <a class="btHeaderA6" href="https://2014.igem.org/Team:Toulouse/Attributions">Attributions</a>
 +
        <ul class="Item sub-nav" style="top : 152px;">
 +
            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Attributions" class="leaf">Attributions</a></li>
 +
            <li class="sub-nav-item" align="left"><a href="https://2014.igem.org/Team:Toulouse/Sponsors" class="leaf">Sponsors</a></li>
 +
        </ul>
 +
    </div>
 +
    <div class="Item" style="left :70%; Z-index:4;" align ="center"  width="60px" >
 +
        <a href="https://2014.igem.org/Main_Page">
 +
        <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px">
 +
        </a>
 +
    </div>
 +
</div>
 +
<!--end navigation menu -->
 +
<tr height="250px"></tr>
 +
</table>
 +
<div class="centering">
-
<td width="45%" valign="top">  
+
<!--TITRE-->
 +
<h1 align="center" style="font-size:50px">
 +
Safety
 +
</h1>
 +
<br>
-
<ul>
+
<!--CITATION-->
-
<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
+
<p align="center"><i>
-
<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
+
"Safety is not just a slogan, it is a way of life"
-
<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
+
</i></p>
-
<li><b>June 9: </b>Submit the About Our Lab form.</li>
+
-
<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
+
-
<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
+
-
<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
+
-
<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
+
-
<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
+
-
<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
+
-
</ul>
+
<br>
-
</td>
+
-
</tr>
+
<!--SUMMARY-->
 +
<p><b>
 +
Summary :</b><br><br>
 +
iGEM Safety<br>
 +
Chassis Organism<br>
 +
New and/or modified coding region<br>
 +
Safety in our lab<br>
 +
</p>
 +
<br>
 +
<!--PARTIE iGEM SAFETY-->
 +
<p>
 +
<b>iGEM Safety</b><br>
 +
Our safety form was approved on September X, 2014 by the iGEM team. We filled in with the help of Nathalie Doubrovine, safety officer at the LISBP.
 +
</p>
 +
<br>
 +
<br>
 +
<!--TABLE CHASSIS ORGANISM-->
 +
<table border="1" width="100%" height="20%" id="Chassis">
 +
<!--1ere ligne-->
<tr>
<tr>
 +
<td><b>Species name (including strain)</b></td>
 +
<td><b>Risk group</b></td>
 +
<td><b>Risk Group Source</b></td>
 +
<td><b>Disease risk to humans ?</b></td>
 +
</tr>
 +
<!--2eme ligne-->
 +
<tr>
 +
<td><i>Escherichia coli MC1061</i></td>
 +
<td>1</td>
 +
<td><a href="http://www.dsmz.de/catalogues/details/culture/DSM-7140.html">DSMZ</a>
 +
<td>No. These organisms do not cause disease in healthy adult humans. (However, they might cause disease in young children, elderly people, or people with immune system deficiencies.)</td>
 +
</tr>
 +
<!--3eme ligne-->
 +
<tr>
 +
<td><i>Bacillus subtilis 168</i></td>
 +
<td>1</td>
 +
<td><a href="http://www.dsmz.de/catalogues/details/culture/DSM-23778.html">DSMZ</a>
 +
<td>No. These organisms do not cause disease in healthy adult humans. (However, they might cause disease in young children, elderly people, or people with immune system deficiencies.)</td>
 +
</tr>
 +
<!--4eme ligne-->
 +
<tr>
 +
<td><i>Aspergillus brasiliensis 246.65 CBS</i></td>
 +
<td>1</td>
 +
<td><a href="http://www.dsmz.de/catalogues/details/culture/DSM-63263.html">DSMZ</a>
 +
<td>No. These organisms do not cause disease in healthy adult humans. (However, they might cause disease in young children, elderly people, or people with immune system deficiencies.)</td>
 +
</tr>
 +
<!--5eme ligne-->
 +
<tr>
 +
<td><i>Chaetomium globosum 148.51 CBS</i></td>
 +
<td>1</td>
 +
<td><a href="http://www.dsmz.de/catalogues/details/culture/DSM-1962.html">DSMZ</a>
 +
<td>No. These organisms do not cause disease in healthy adult humans. (However, they might cause disease in young children, elderly people, or people with immune system deficiencies.)</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<br>
 +
<!--TABLE NEW AND/OR MODIFIED CODING REGION-->
 +
<table border="1" width="100%" height="20%" id="Coding">
 +
<!--1ere ligne-->
 +
<tr>
 +
<td><b>Part number/name</b></td>
 +
<td><b>Natural function of part</b></td>
 +
<td><b>How did you acquire it?</b></td>
 +
<td><b>How will you use it?</b></td>
 +
</tr>
 +
<!--2eme ligne-->
 +
<tr>
 +
<td>RBS (K090505) +  Chimiotaxis + Terminator(B0015)</td>
 +
<td>Chimiotaxis code for a chimeric protein (based on McpA (1-34/283-661) from B.sub and VCD_00306(28-315) from V.cholerae)</td>
 +
<td>We order this gene from a synthesis company (Eurofins)</td>
 +
<td>This part allows the bacterium to reach the fungus</td>
 +
</tr>
 +
<!--3eme ligne-->
 +
<tr>
 +
<td>RBS (K090505) + binding  + Terminator(B0015)</td>
 +
<td>"Binding" code for a chimeric binding protein (based on LYCT (1-318) from B.sub and GBPA (324-384) from V.Cholerae) with a linker between.</td>
 +
<td>We order this gene from a synthesis company (Eurofins)</td>
 +
<td>This part allows the bacterium to fix the fungus</td>
 +
</tr>
 +
<!--4eme ligne-->
 +
<tr>
 +
<td>RBS (K090505) + GAFP1</td>
 +
<td>GAFP1 code for a antifungal. GAFP1 come from arachis hypogaea (Peanut)</td>
 +
<td>We order this gene from a synthesis company (Eurofins)</td>
 +
<td>This part produces a fungicide. By this way, the bacteria is able to kill fungus.</td>
 +
</tr>
 +
<!--5eme ligne-->
 +
<tr>
 +
<td>RBS (K090505) + D4E1  + Terminator(B0015)</td>
 +
<td>D4E1 code for a antifungal. DAE1 is a synthetic gene</td>
 +
<td>We order this gene from a synthesis company (Eurofins)</td>
 +
<td>This part produces a fungicide. By this way, the bacteria is able to kill fungus.</td>
 +
</tr>
 +
<!--6eme ligne-->
 +
<tr>
 +
<td>Promoter(K823003) + (K1364000: RBS (K090505) +  Chimiotaxis + Terminator(B0015))</td>
 +
<td>Promoter Pveg, and Chimiotaxis part</td>
 +
<td>We made it</td>
 +
<td>This part allows the bacterium to reach the fungus</td>
 +
</tr>
 +
<!--7eme ligne-->
 +
<tr>
 +
<td>Promoter(K823003) + (K1364001: RBS (K090505) +  Binding + Terminator(B0015))</td>
 +
<td>Promoter Pveg, and binding part</td>
 +
<td>We made it</td>
 +
<td>This part allow the bacterium to fix the fungus</td>
 +
</tr>
 +
<!--8eme ligne-->
 +
<tr>
 +
<td>(K1364004: Promoter(K823003) + RBS (K090505) +  Chimiotaxis + Terminator(B0015))+(K1364005: Promoter(K823003) + RBS (K090505) +  Binding + Terminator(B0015))</td>
 +
<td>Double expression cassette</td>
 +
<td>We made it</td>
 +
<td>This part allows the bacterium to reach and bind the fungus</td>
 +
</tr>
 +
<!--9eme ligne-->
 +
<tr>
 +
<td>Promoteur(K823003) + (K1364002 : RBS (K090505) + GAFP1)</td>
 +
<td>Promoter Pveg, and GAFP1 part</td>
 +
<td>We made it</td>
 +
<td>This part produces a fungicide. By this way, the bacterium is able to kill fungus</td>
 +
</tr>
 +
<!--10eme ligne-->
 +
<tr>
 +
<td>(K1364002 : Promoteur(K823003) + RBS (K090505) + GAFP1) + Terminator (B0015)</td>
 +
<td>Promoter Pveg, and GAFP1 part with a double terminator</td>
 +
<td>We made it</td>
 +
<td>This part produces a fungicide. By this way, the bacterium is able to kill fungus</td>
 +
</tr>
 +
<!--11eme ligne-->
 +
<tr>
 +
<td>Promoter(K823003) + (K1364003 : RBS (K090505) + D4E1  + Terminator(B0015))</td>
 +
<td>Promoter(K823003) + (K1364003 : RBS (K090505) + D4E1  + Terminator(B0015))</td>
 +
<td>We made it</td>
 +
<td>This part produces a fungicide. By this way, the bacterium is able to kill fungus</td>
 +
</tr>
 +
<!--12eme ligne-->
 +
<tr>
 +
<td>K71364010: Promoter + RBS + EcAMP) + terminator(B0015)</td>
 +
<td>Promoter Pveg, RBS SpoVG, Antifungal EcAMP, double terminator</td>
 +
<td>We made it</td>
 +
<td>This part produces a fungicide. By this way, the bacterium is able to kill fungus</td>
 +
</tr>
 +
<!--13eme ligne-->
 +
<tr>
 +
<td>K1364002 : RBS  + GAFP1  ) + (K1364003:  RBS + D4E1 + terminator)</td>
 +
<td>RBS consensus, Antifungal GAFP1, RBS consensus, Antifungal D4E1, double terminator</td>
 +
<td>We made it</td>
 +
<td>This part produces a fungicide. By this way, the bacterium is able to kill fungus</td>
 +
</tr>
 +
<!--14eme ligne-->
 +
<tr>
 +
<td>Promoter(K823003)+(K1364012 : RBS  + GAFP1  +  RBS + D4E1 + terminator)</td>
 +
<td>Promoter Pveg, RBS consensus, Antifungal GAFP1, RBS consensus, Antifungal D4E1, double terminator</td>
 +
<td>We made it</td>
 +
<td>This part produces a fungicide. By this way, the bacterium is able to kill fungus.</td>
 +
</tr>
 +
<!--15eme ligne-->
 +
<tr>
 +
<td>(K71364010: Promoter + RBS + EcAMP) + (K1364012 : RBS  + GAFP1  +  RBS + D4E1 + terminator)</td>
 +
<td>Promoter Pveg,  RBS SpoVG, Antifungal EcAMP, RBS consensus, Antifungal GAFP1, RBS consensus, Antifungal D4E1, double terminator</td>
 +
<td>We made it</td>
 +
<td>This part produces a fungicide. By this way, the bacterium is able to kill fungus.</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<!--ETHIDIUM BROMIDE-->
 +
<p><b>Ethidium bromide</b><br>
 +
All along our project, we use non-pathogenic strains and non-toxic product, except the EtBr.
 +
We have a dark room dedicated to the  use of EtBr and UV. This room is key-closed and everyone entering into the room must wear gloves, glasses and lab coat. Everything which is in contact directly with something in this room has to stay here.
 +
Two  specific garbages are dedicated to the gloves and the agarose gels contaminated
 +
</p>
 +
<br>
-
</table>
+
<!--SAFETY PRINCIPLES-->
 +
<p><b>Basic safety principles</b><br>
 +
All along our project, we use non-pathogenic strains and non-toxic product, except the EtBr.
 +
However, we have to apply the basic safety principles into a laboratory room:
 +
- It is forbidden to smoke in all the INSA&apos;s rooms.
 +
- It is forbidden to drink and eat in the laboratory rooms.
 +
- It is compulsory to wear a closed lab coat in cotton.
 +
- It is compulsory to wear closed shoes.
 +
- Long hair must be tied back
 +
- Oral pipetting of any substance is prohibited in any laboratory
 +
</p>
 +
 
 +
<br>
 +
 
 +
<!--CITATION-->
 +
<p align="center"><i>
 +
"Precaution is better than cure"
 +
</i></p>
 +
 
 +
<br>
 +
 
 +
<!--SAFETY PRINCIPLES-->
 +
<p><b>Basic safety principles</b><br>
 +
There is also others precaution when working with biological organisms.
 +
- We need to wash our hands regularly.
 +
- It is compulsory to wear gloves except when working with an electric burner.
 +
- In some case (UV light, projection risk), it is compulsory to wear protection glasses.
 +
Indeed, different machine are used to keep a sterile area.
 +
</p>
 +
 
 +
<br>
 +
 
 +
<!--CITATION-->
 +
<p align="center"><i>
 +
"If you don&apos;t think it&apos;s safe, it&apos;s probably isn&apos;t"
 +
</i></p>
 +
 
 +
 
 +
 
 +
<!-- footer -->
 +
<center>
 +
<div id="Footer" >
 +
<table align="center" style="width : 100%; background-color:transparent;" bgColor="#99FF99">
 +
    <td width="5px"></td>
 +
    <td style="width: 35px;" align="center">
 +
        <a href="https://www.facebook.com/iGEM.Toulouse.2014?ref_type=bookmark"><img src="https://static.igem.org/mediawiki/2013/f/fc/Insa-toulouse2013-iconfacebook.png" alt="Facebook" /></a>
 +
        <br> <br>
 +
        <a href="https://twitter.com/iGEM_Toulouse"><img src="https://static.igem.org/mediawiki/2013/7/7b/Insa-toulouse2013-icontwitter.png" alt="Twitter" /></a>
 +
        <br>
 +
    </td>
 +
    <td width="15px"></td>
 +
    <td>
 +
        <p><b> iGEM Toulouse</b><br><address>
 +
        INSA Toulouse <br>135, Avenue de Rangueil <br>
 +
        31400 TOULOUSE <br></address>
 +
        <br>e-mail: <span><a href="mailto:igemtoulouse2014@gmail.com"> igemtoulouse2014@gmail.com</a></span></p>
 +
    </td>
 +
    <td align="right">
 +
        <a href="https://2013.igem.org/Main_Page">
 +
            <img style="width:170px" src="https://static.igem.org/mediawiki/igem.org/1/17/IGEM_basic_Logo_white_stylized.png"/img>
 +
        </a>
 +
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 +
  </table>
 +
</div>
 +
</center>
 +
</div>
</html>
</html>

Revision as of 12:38, 28 August 2014

header background

Safety


"Safety is not just a slogan, it is a way of life"


Summary :

iGEM Safety
Chassis Organism
New and/or modified coding region
Safety in our lab


iGEM Safety
Our safety form was approved on September X, 2014 by the iGEM team. We filled in with the help of Nathalie Doubrovine, safety officer at the LISBP.



Species name (including strain) Risk group Risk Group Source Disease risk to humans ?
Escherichia coli MC1061 1 DSMZ No. These organisms do not cause disease in healthy adult humans. (However, they might cause disease in young children, elderly people, or people with immune system deficiencies.)
Bacillus subtilis 168 1 DSMZ No. These organisms do not cause disease in healthy adult humans. (However, they might cause disease in young children, elderly people, or people with immune system deficiencies.)
Aspergillus brasiliensis 246.65 CBS 1 DSMZ No. These organisms do not cause disease in healthy adult humans. (However, they might cause disease in young children, elderly people, or people with immune system deficiencies.)
Chaetomium globosum 148.51 CBS 1 DSMZ No. These organisms do not cause disease in healthy adult humans. (However, they might cause disease in young children, elderly people, or people with immune system deficiencies.)


Part number/name Natural function of part How did you acquire it? How will you use it?
RBS (K090505) + Chimiotaxis + Terminator(B0015) Chimiotaxis code for a chimeric protein (based on McpA (1-34/283-661) from B.sub and VCD_00306(28-315) from V.cholerae) We order this gene from a synthesis company (Eurofins) This part allows the bacterium to reach the fungus
RBS (K090505) + binding + Terminator(B0015) "Binding" code for a chimeric binding protein (based on LYCT (1-318) from B.sub and GBPA (324-384) from V.Cholerae) with a linker between. We order this gene from a synthesis company (Eurofins) This part allows the bacterium to fix the fungus
RBS (K090505) + GAFP1 GAFP1 code for a antifungal. GAFP1 come from arachis hypogaea (Peanut) We order this gene from a synthesis company (Eurofins) This part produces a fungicide. By this way, the bacteria is able to kill fungus.
RBS (K090505) + D4E1 + Terminator(B0015) D4E1 code for a antifungal. DAE1 is a synthetic gene We order this gene from a synthesis company (Eurofins) This part produces a fungicide. By this way, the bacteria is able to kill fungus.
Promoter(K823003) + (K1364000: RBS (K090505) + Chimiotaxis + Terminator(B0015)) Promoter Pveg, and Chimiotaxis part We made it This part allows the bacterium to reach the fungus
Promoter(K823003) + (K1364001: RBS (K090505) + Binding + Terminator(B0015)) Promoter Pveg, and binding part We made it This part allow the bacterium to fix the fungus
(K1364004: Promoter(K823003) + RBS (K090505) + Chimiotaxis + Terminator(B0015))+(K1364005: Promoter(K823003) + RBS (K090505) + Binding + Terminator(B0015)) Double expression cassette We made it This part allows the bacterium to reach and bind the fungus
Promoteur(K823003) + (K1364002 : RBS (K090505) + GAFP1) Promoter Pveg, and GAFP1 part We made it This part produces a fungicide. By this way, the bacterium is able to kill fungus
(K1364002 : Promoteur(K823003) + RBS (K090505) + GAFP1) + Terminator (B0015) Promoter Pveg, and GAFP1 part with a double terminator We made it This part produces a fungicide. By this way, the bacterium is able to kill fungus
Promoter(K823003) + (K1364003 : RBS (K090505) + D4E1 + Terminator(B0015)) Promoter(K823003) + (K1364003 : RBS (K090505) + D4E1 + Terminator(B0015)) We made it This part produces a fungicide. By this way, the bacterium is able to kill fungus
K71364010: Promoter + RBS + EcAMP) + terminator(B0015) Promoter Pveg, RBS SpoVG, Antifungal EcAMP, double terminator We made it This part produces a fungicide. By this way, the bacterium is able to kill fungus
K1364002 : RBS + GAFP1 ) + (K1364003: RBS + D4E1 + terminator) RBS consensus, Antifungal GAFP1, RBS consensus, Antifungal D4E1, double terminator We made it This part produces a fungicide. By this way, the bacterium is able to kill fungus
Promoter(K823003)+(K1364012 : RBS + GAFP1 + RBS + D4E1 + terminator) Promoter Pveg, RBS consensus, Antifungal GAFP1, RBS consensus, Antifungal D4E1, double terminator We made it This part produces a fungicide. By this way, the bacterium is able to kill fungus.
(K71364010: Promoter + RBS + EcAMP) + (K1364012 : RBS + GAFP1 + RBS + D4E1 + terminator) Promoter Pveg, RBS SpoVG, Antifungal EcAMP, RBS consensus, Antifungal GAFP1, RBS consensus, Antifungal D4E1, double terminator We made it This part produces a fungicide. By this way, the bacterium is able to kill fungus.

Ethidium bromide
All along our project, we use non-pathogenic strains and non-toxic product, except the EtBr. We have a dark room dedicated to the use of EtBr and UV. This room is key-closed and everyone entering into the room must wear gloves, glasses and lab coat. Everything which is in contact directly with something in this room has to stay here. Two specific garbages are dedicated to the gloves and the agarose gels contaminated


Basic safety principles
All along our project, we use non-pathogenic strains and non-toxic product, except the EtBr. However, we have to apply the basic safety principles into a laboratory room: - It is forbidden to smoke in all the INSA&apos;s rooms. - It is forbidden to drink and eat in the laboratory rooms. - It is compulsory to wear a closed lab coat in cotton. - It is compulsory to wear closed shoes. - Long hair must be tied back - Oral pipetting of any substance is prohibited in any laboratory


"Precaution is better than cure"


Basic safety principles
There is also others precaution when working with biological organisms. - We need to wash our hands regularly. - It is compulsory to wear gloves except when working with an electric burner. - In some case (UV light, projection risk), it is compulsory to wear protection glasses. Indeed, different machine are used to keep a sterile area.


"If you don&apos;t think it&apos;s safe, it&apos;s probably isn&apos;t"

Retrieved from "http://2014.igem.org/Team:Toulouse/Safety"