Team:Toulouse/Result/experimental-results

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- The same process was made with xylose as a positive control.<br>
- The same process was made with xylose as a positive control.<br>
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<i>NB: According to the paper "Chemotaxis towards sugars by </i>B. subtilis", (Ordal et al., 1979),<i> glucose and xylose have the same attractant power. We have privileged a positive control instead of a negative one as we were not sure that this system was efficient.</i><br>
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<i>NB: According to the paper "Chemotaxis towards sugars by </i>B. subtilis, (Ordal et al., 1979),<i> glucose and xylose have the same attractant power. We have privileged a positive control instead of a negative one as we were not sure that this system was efficient.</i><br>
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- The system was kept straight for 2 hours. Every 40 minutes, samples from each were removed and streaked on solid medium (dilution 1/1,000) in order to estimate the bacterial concentration.</p>
- The system was kept straight for 2 hours. Every 40 minutes, samples from each were removed and streaked on solid medium (dilution 1/1,000) in order to estimate the bacterial concentration.</p>
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<p class="title3">Improvement of the second tips capillary system</p>
<p class="title3">Improvement of the second tips capillary system</p>
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<p class="texte">However this system was not optimal it is why we decided to use blu tack instead of parafilm: <br></p>
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<p class="texte">However this system was not optimal it is why we decided to use blu-tack instead of parafilm: <br></p>
<center><img src="https://static.igem.org/mediawiki/2014/4/42/Chemotaxis_-_pipette_and_blu_tack.png"></center>
<center><img src="https://static.igem.org/mediawiki/2014/4/42/Chemotaxis_-_pipette_and_blu_tack.png"></center>
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<p class="legend">Figure 17: Attachment of WT <i>B. subtilis</i> and engineered bacterium to chitin. The <span style="color:#0000FF">WT bacteria</span> or <span style="color:#FF0000">the bacteria with the binding system</span> concentrations have been determined during the different steps of the binding test. The stars represent a significant difference observed with a Student test with p<0.05.
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<p class="legend">Figure 17: Attachment of WT <i>Bacillus subtilis</i> and engineered bacterium to chitin. The <span style="color:#0000FF">WT bacteria</span> or <span style="color:#FF0000">the bacteria with the binding system</span> concentrations have been determined during the different steps of the binding test. The stars represent a significant difference observed with a Student test with p<0.05.
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<p class="texte">We then performed a wash step on the chitin beads. We measured the release of bacteria on the washing solution. When our bacterium has the binding module (Right on Figure 20), there is less release than without the module (Right on Figure 20). Therefore, the engineered bacterium is retained by the beads.</p>
<p class="texte">We then performed a wash step on the chitin beads. We measured the release of bacteria on the washing solution. When our bacterium has the binding module (Right on Figure 20), there is less release than without the module (Right on Figure 20). Therefore, the engineered bacterium is retained by the beads.</p>
<center><img src="https://static.igem.org/mediawiki/2014/9/97/Photo_lavage_microscopie.png" width="45%"></center>
<center><img src="https://static.igem.org/mediawiki/2014/9/97/Photo_lavage_microscopie.png" width="45%"></center>
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<p class="legend">Figure 20: Microscopic view of elution fraction of WT <i>B. subtilis</i> (Left) and engineered bacterium (Right).  
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<p class="legend">Figure 20: Microscopic view of elution fraction of WT <i>Bacillus subtilis</i> (Left) and engineered bacterium (Right).  
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<center><img style="width:215px;" img src="https://static.igem.org/mediawiki/parts/a/af/In_planta.jpg" style="margin-top:5px"/></center>
<center><img style="width:215px;" img src="https://static.igem.org/mediawiki/parts/a/af/In_planta.jpg" style="margin-top:5px"/></center>
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<p class="legend"> Figure 23: Injection of antifungal <i>B. subtilis</i> in a model plant</p>
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<p class="legend"> Figure 23: Injection of antifungal <i>Bacillus subtilis</i> in a model plant</p>
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Latest revision as of 03:13, 18 October 2014