Team:Toulouse/Project/Spreading

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<p class="title1">Survival in the environment: <i>B. subtilis</i> that is proline auxotrophic</p>
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<p class="title1">Survival in the environment: <i>Bacillus subtilis</i> that is proline auxotrophic</p>
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<p class="texte">SubtiTree will live in sap tree, thus we will use an endophyte <I>B. subtilis</I> strain. In order to contain our bacteria in this  
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<p class="texte">SubtiTree will live in sap tree, thus we will use an endophyte <I>Bacillus subtilis</I> strain. In order to contain our bacteria in this  
area during a short period of time, we think about modifying some of its survival characteristics. To turn the bacterium growth dependant on the presence  
area during a short period of time, we think about modifying some of its survival characteristics. To turn the bacterium growth dependant on the presence  
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inside the tree (and therefore avoid spreading in the environment), we planned to use a <i>B.subtilis</i> strain that is proline auxotrophic. The bacterium
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inside the tree (and therefore avoid spreading in the environment), we planned to use a <i>B. subtilis</i> strain that is proline auxotrophic. The bacterium
  should then be unable to synthesize this essential amino acid. Proline is the most abundant amino acid in the phloem sap. If the bacterium is in the sap,
  should then be unable to synthesize this essential amino acid. Proline is the most abundant amino acid in the phloem sap. If the bacterium is in the sap,
  it should grow normally without any deficiency, but if it escapes from the tree and <i>a fortiori</i> from the sap, it will not be able to survive for a  
  it should grow normally without any deficiency, but if it escapes from the tree and <i>a fortiori</i> from the sap, it will not be able to survive for a  
  long time as proline is present only in very low quantities in the soil.  
  long time as proline is present only in very low quantities in the soil.  
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Auxotrophic <i>B.subtilis</i> strains already exist and are indexed in the databases BGSC (Bacillus Genetic Stock Center).</p>
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Auxotrophic <i>B. subtilis</i> strains already exist and are indexed in the databases BGSC (Bacillus Genetic Stock Center).</p>
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<p class="title1">Preventing sporulation of <i>B. subtilis</i></p>
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<p class="title1">Preventing sporulation of <i>Bacillus subtilis</i></p>
<p class="texte">
<p class="texte">
It is known that endophyte bacteria must sporulate to survive during the winter. We planned to limit Subtitree's lifespan to only one season.
It is known that endophyte bacteria must sporulate to survive during the winter. We planned to limit Subtitree's lifespan to only one season.
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To control any unwanted long-term development of SubtiTree, our strain should therefore be unable to sporulate. Thus, during fall, when the sap become  
To control any unwanted long-term development of SubtiTree, our strain should therefore be unable to sporulate. Thus, during fall, when the sap become  
less nutritious and the temperature is lower, the engineered bacterium will die and not pass through the following winter. This question has been studied in our
less nutritious and the temperature is lower, the engineered bacterium will die and not pass through the following winter. This question has been studied in our
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  <a= href "https://2014.igem.org/Team:Toulouse/Modelling" Modeling part> </a>.<br/>
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  <a= href "https://2014.igem.org/Team:Toulouse/Modelling"> Modeling part </a>.<br/>
In addition, deleting all the engineered bacterial community every year puts a brake on the evolution due to random mutations,
In addition, deleting all the engineered bacterial community every year puts a brake on the evolution due to random mutations,
  thus allowing a better faith on the genetic constructions.
  thus allowing a better faith on the genetic constructions.
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<p class="texte">
<p class="texte">
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While we have not constructed yet these modules, we definitely think that the measures that we designed should render the use of SubtiTree acceptable  
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While we have not constructed yet these modules, we definitely think that the elaborated strategies we designed should render the use of SubtiTree acceptable  
in real conditions.   
in real conditions.   
</p>
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<ul>
<ul>
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<li class="tree"><p class="texte">S. Dinant, J.L. Bonnemain, C. Girousse, J. Kehr. <b> Phloem sap intricacy and interplay with aphid feeding.</b>C R Biol. 2010 Jun-Jul;333(6-7):504-15. doi: 10.1016/j.crvi.2010.03.008. Epub 2010 May 14.</p></li>
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<li class="tree"><p class="texte">S. Dinant, J.L. Bonnemain, C. Girousse, and J. Kehr. <b> Phloem sap intricacy and interplay with aphid feeding.</b>C R Biol. 2010 Jun-Jul;333(6-7):504-15. doi: 10.1016/j.crvi.2010.03.008. Epub 2010 May 14.</p></li>
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<li class="tree"><p class="texte"> Z.N. Senwo and M.A. Tabatabai. <b> Amino acid composition of soil organic matter.</b> Biology and Fertility of SoilsFebruary 1998, Volume 26, Issue 3, pp 235-242 </p></li>
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<li class="tree"><p class="texte"> Z.N. Senwo, and M.A. Tabatabai. <b> Amino acid composition of soil organic matter.</b> Biology and Fertility of SoilsFebruary 1998, Volume 26, Issue 3, pp 235-242 </p></li>
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<li class="tree"><p class="texte">A.M. Guérout-Fleury, N. Frandsen and P. Stragier <b> Plasmids for ectopic integration in Bacillus subtilis.</b> Gene. 1996 Nov 21;180(1-2):57-61.</p></li>
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<li class="tree"><p class="texte">A.M. Guérout-Fleury, N. Frandsen, and P. Stragier. <b> Plasmids for ectopic integration in <i>Bacillus subtilis</i>.</b> Gene. 1996 Nov 21;180(1-2):57-61.</p></li>
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<li class="tree"><p class="texte">G. Shang, X. Liu, D. Lu, J. Zhang, N. Li, C. Zhu, S. Liu, Q. Yu, Y. Zhao and L. Gu. <b> Structural insight into how Pseudomonas aeruginosa peptidoglycanhydrolase Tse1 and its immunity protein Tsi1 function.</b> Biochem J. 2012 Dec 1;448(2):201-11. doi: 10.1042/BJ20120668.</p></li>
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<li class="tree"><p class="texte">G. Shang, X. Liu, D. Lu, J. Zhang, N. Li, C. Zhu, S. Liu, Q. Yu, Y. Zhao, and L. Gu. <b> Structural insight into how <i>Pseudomonas aeruginosa</i> peptidoglycanhydrolase Tse1 and its immunity protein Tsi1 function.</b> Biochem J. 2012 Dec 1;448(2):201-11. doi: 10.1042/BJ20120668.</p></li>
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<li class="tree"><p class="texte">W.Z. Hua, C. S. Yong and X.T. Ren<b>Biology and chemistry of endophytes.</b> Nat. Prod. Rep., 2006, 23, 753–771, 753</p></li>
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<li class="tree"><p class="texte">W.Z. Hua, C. S. Yong, and X.T. Ren. <b>Biology and chemistry of endophytes.</b> Nat. Prod. Rep., 2006, 23, 753–771, 753</p></li>
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Latest revision as of 03:02, 18 October 2014