Team:Toulouse/Project/Spreading

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<p class="texte">SubtiTree will live in sap tree, thus we will use an endophyte <I>B. subtilis</I> strain. In order to contain our bacteria in this area during a short period of time, we thought of modifying some of its survival characteristics. To make the bacterium growth dependant on the presence of the tree (and therefore avoid spreading in the environment), we planned to use a <i>B.subtilis</i> strain auxotroph for proline. The bacterium should then be unable to synthesize this essential amino acid. Proline is the most abundant amino acid in the phloem sap. If the bacterium is in the sap, it should grow normally without any deficiency, but if it escapes from the tree and <i>a fortiori</i> from the sap, it will not be able to survive for a long time (proline is present only in very low quantities in the ground).  
<p class="texte">SubtiTree will live in sap tree, thus we will use an endophyte <I>B. subtilis</I> strain. In order to contain our bacteria in this area during a short period of time, we thought of modifying some of its survival characteristics. To make the bacterium growth dependant on the presence of the tree (and therefore avoid spreading in the environment), we planned to use a <i>B.subtilis</i> strain auxotroph for proline. The bacterium should then be unable to synthesize this essential amino acid. Proline is the most abundant amino acid in the phloem sap. If the bacterium is in the sap, it should grow normally without any deficiency, but if it escapes from the tree and <i>a fortiori</i> from the sap, it will not be able to survive for a long time (proline is present only in very low quantities in the ground).  
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Auxotroph <i>B.sutbilis</i> strains already exist and are indexed in databases as BGSC (Bacillus Genetic Stock Center), therefore they should be easy to find.</p> DIRE QUE CE N'EST PAS CE QU'ON A UTILISE??
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Auxotroph <i>B.sutbilis</i> strains already exist and are indexed in databases as BGSC (Bacillus Genetic Stock Center), therefore they should be easy to find.</p>
<p class="title1">Preventing sporulation of <i>B. subtilis</i></p>
<p class="title1">Preventing sporulation of <i>B. subtilis</i></p>

Revision as of 12:29, 17 October 2014