Team:Toulouse/Project/Spreading

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It is known that endophyte bacteria must sporulate to survive to winter. In order to limit the spreading of our bacterium, we decided to limit its lifespan to only one season. The bacteria should be injected in spring, grow during the summer and finally should be inactivated in fall.<br\>  
It is known that endophyte bacteria must sporulate to survive to winter. In order to limit the spreading of our bacterium, we decided to limit its lifespan to only one season. The bacteria should be injected in spring, grow during the summer and finally should be inactivated in fall.<br\>  
<i>Bacillus subtilis</i> is a sporing bacterium : sporulation enable the microorganism to handle very harsh conditions and to spread tree to tree. Indeed, a spore is a very resistant form that is adapted for unfavorable conditions and for dispersal.<br/>  
<i>Bacillus subtilis</i> is a sporing bacterium : sporulation enable the microorganism to handle very harsh conditions and to spread tree to tree. Indeed, a spore is a very resistant form that is adapted for unfavorable conditions and for dispersal.<br/>  
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To keep the control on the development of SubtiTree, our strain should therefore be non-sporingh. We chose a <I>B.subtilis</I> strain without late genes of sporulation. Thus, after a season of treatment, the sape become less nutritious, the temperature is low and the engineered bacterium cannot survive the following winter.<br/>
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To keep the control on the development of SubtiTree, our strain should therefore be non-sporing. We chose a <I>B.subtilis</I> strain without late genes of sporulation. Thus, after a season of treatment, the sape become less nutritious, the temperature is low and the engineered bacterium cannot survive the following winter.<br/>
In addition, deleting all the engineered bacterial community every year puts a brake on the evolution due to random mutation, thus it allows to keep control on the genetic constructions.
In addition, deleting all the engineered bacterial community every year puts a brake on the evolution due to random mutation, thus it allows to keep control on the genetic constructions.
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Revision as of 12:46, 13 October 2014