Team:Toronto

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<!--<h1><span>Biocontainment: a novel approach</span></h1>-->
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<h1><span>Where’s Plasmo?</span></h1>
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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Our project can be compared to the concept of the popular children’s book, “Where’s Waldo?” that consists of pages depicting large images of almost identical looking people and characters in various situations, and the challenge for readers is to find Waldo somewhere in this crowd. Waldo wears a red and white striped shirt and is often quite hard to recognize in the sea of stripes, red, and white. Plasmo is the bacterial version of Waldo.
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Toronto&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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Our biocontainment project is based upon the idea of using a plasmid-loss genetic safeguard. Normally, “kill-switches” are used as the go-to mechanism for genetic safeguards. However, kill-switches are problematic because they impose selective pressure on a population of synthetic organisms to evolve resistance to kill-switches. In the aftermath of a kill switch activation, only the resistant mutants remain (represented by Plasmo) and they replicate to form a large population of Plasmos.
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In our alternative approach, the <b>plasmid-loss genetic safeguard system</b>, the genetically engineered traits are removed from the population of bacteria using a <b>"self-deleting" CRISPR/Cas9 plasmid</b>. In the end, the population with it's plasmid deleted is indistinguishable from the wild-type population. Since there are still so many wild-type bacteria in the population, the Plasmos are lost among them. They are so few compared to the wild-type bacteria that you can barely find them anymore!
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We propose this as a safer mechanism for using synthetic organisms outside of laboratory settings, so that the environment is not overrun by tons of Plasmos!
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<a href="https://2014.igem.org/Team:Toronto"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:Toronto/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Toronto"style="color:#000000"> Official Team Profile </a></td>
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2014 University of Toronto iGEM
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<li><a href="https://2014.igem.org/Team:Toronto">Home</a> </li>
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<li><a href="https://2014.igem.org/Team:Toronto/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Toronto">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:Toronto/Notebook">Notebook</a> </li>
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<li><a href="https://2014.igem.org/Team:Toronto/Safety">Safety</a> </li>
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a> lists. </p>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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Latest revision as of 03:55, 18 October 2014


Where’s Plasmo?

Our project can be compared to the concept of the popular children’s book, “Where’s Waldo?” that consists of pages depicting large images of almost identical looking people and characters in various situations, and the challenge for readers is to find Waldo somewhere in this crowd. Waldo wears a red and white striped shirt and is often quite hard to recognize in the sea of stripes, red, and white. Plasmo is the bacterial version of Waldo.

Our biocontainment project is based upon the idea of using a plasmid-loss genetic safeguard. Normally, “kill-switches” are used as the go-to mechanism for genetic safeguards. However, kill-switches are problematic because they impose selective pressure on a population of synthetic organisms to evolve resistance to kill-switches. In the aftermath of a kill switch activation, only the resistant mutants remain (represented by Plasmo) and they replicate to form a large population of Plasmos.

In our alternative approach, the plasmid-loss genetic safeguard system, the genetically engineered traits are removed from the population of bacteria using a "self-deleting" CRISPR/Cas9 plasmid. In the end, the population with it's plasmid deleted is indistinguishable from the wild-type population. Since there are still so many wild-type bacteria in the population, the Plasmos are lost among them. They are so few compared to the wild-type bacteria that you can barely find them anymore!

We propose this as a safer mechanism for using synthetic organisms outside of laboratory settings, so that the environment is not overrun by tons of Plasmos!