Team:Technion-Israel/Project
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Bio-detectors have been a big part of the iGEM projects ever since the competition first started, it's easy to see why: One of the simplest systems to build using our current tools for synthetic biology is a simple Input→Output "linear" (Promoter→Signaling Gene) bio-detector but this method has a major flaw:<br> | Bio-detectors have been a big part of the iGEM projects ever since the competition first started, it's easy to see why: One of the simplest systems to build using our current tools for synthetic biology is a simple Input→Output "linear" (Promoter→Signaling Gene) bio-detector but this method has a major flaw:<br> |
Revision as of 09:37, 14 October 2014
The Project
"Success consists of going from failure to failure without loss of enthusiasm."--Winston Churchill
Bio-detectors have been a big part of the iGEM projects ever since the competition first started, it's easy to see why: One of the simplest systems to build using our current tools for synthetic biology is a simple Input→Output "linear" (Promoter→Signaling Gene) bio-detector but this method has a major flaw:
In order to get a detection signal that's visible to the naked eye, we must have a LOT of bacteria change color (or any other signal). With the linear approach we find ourselves needing high concentration of the detected material for our system to be effective!
Now, while this issue is far from new and various teams have already tried to tackle this exact problem before, our team worked for a year on a new approach utilizing things like quorum sensing for inter-bacteria communication and signal amplification which is possible thanks to our creation of a synthetic bio-film using a revolutionary organic molecule called Azobenzene, resulting in what we refer to as a 'smart, self assembling material'.
Neetd lots of editing
Beta System
(1) Our bacteria has two main features - one is a sensor and the second is azobenzene attached to the LPS. When the bacteria detects a substance it changes color by producing GFP
(2) The radiation emerging from the bacteria causes the azobenzene molecules to change conformation to a "sticky" form
(3) The azobenzene molecules cause the bacteria to aggregate by forming bonds through azobenzene, allowing fast diffusion of communication molecules
Introfuction
Gene Deletion
Failed to delete ackA-pta genes
Neetd lots of editing
Here are all the protocols we used in the project
to view the files you will need Adobe Acrobat Reader or similar
Chemical Trasformation
DNA Kit Plate Instructions
Z-Competent™ cells and Mix&Go
Gel/PCR Extraction
Gel Preparation
Gibson Assembly
Glycerol stock
LB, BA, SOB, SOC
Ligation
Presto Mini-prep
PCR
Phosphorylation and blunt ligation
Restriction enzyme
Electroporation Trasformation
Gene deletion using λ red
Genome extaraction
to view the files you will need Adobe Acrobat Reader or similar
Gene Deletion & Histidine Kinase
This is Rebecca's and Karen's lab notebook for gene deletion attempts and TaZ biobrick building.
Gate Construst
These are a few notebooks arranged together of all gate constructs. Lab work done by Tal, Rica, Ronen, Shira, Noa, Alex and Ittai.
Azobenzene
This is the lab notebook of all Azobenzene lab work done by Faris, our chemist. This notebook is all chemistry.
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