Team:Technion-Israel/Project
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<center><h2 style="box-shadow:0px 0px 1px 1px rgba(255,255,255,0.25); border-radius:0.35em; font-weight:700; padding:1em;">Alpha System</h2> | <center><h2 style="box-shadow:0px 0px 1px 1px rgba(255,255,255,0.25); border-radius:0.35em; font-weight:700; padding:1em;">Alpha System</h2> | ||
- | + | <p style="font-size: 1.1em;"><br>The Alpha System is the preliminary design we planned for a biosensor that can detect various materials at low concentrations.<br> | |
Unlike other biosensors, our system is meant to be as versatile as possible- with a minor change at the genetic level it can detect molecule A instead of molecule B. That saves us the need to construct an entirely different detector for every dangerous material out there.<br> | Unlike other biosensors, our system is meant to be as versatile as possible- with a minor change at the genetic level it can detect molecule A instead of molecule B. That saves us the need to construct an entirely different detector for every dangerous material out there.<br> | ||
*Maybe a picture of a bun and a heavy metal frightened because of safie* <br> | *Maybe a picture of a bun and a heavy metal frightened because of safie* <br> |
Revision as of 11:19, 13 October 2014
The Project
"Success consists of going from failure to failure without loss of enthusiasm."--Winston Churchill
The Idea
How It works
Neetd lots of editing
Beta System
Beta System
Azobenzene
Histidine Kinase
Introfuction
Gene Deletion
Failed to delete ackA-pta genes
Experiments
Neetd lots of editing
Protocols
Here are all the protocols we used in the project
to view the files you will need Adobe Acrobat Reader or similar
Antibiotics Preparation
Chemical Trasformation
DNA Kit Plate Instructions
Z-Competent™ cells and Mix&Go
Gel/PCR Extraction
Gel Preparation
Gibson Assembly
Glycerol stock
LB, BA, SOB, SOC
Ligation
Presto Mini-prep
PCR
Phosphorylation and blunt ligation
Restriction enzyme
Electroporation Trasformation
Gene deletion using λ red
Genome extaraction
Chemical Trasformation
DNA Kit Plate Instructions
Z-Competent™ cells and Mix&Go
Gel/PCR Extraction
Gel Preparation
Gibson Assembly
Glycerol stock
LB, BA, SOB, SOC
Ligation
Presto Mini-prep
PCR
Phosphorylation and blunt ligation
Restriction enzyme
Electroporation Trasformation
Gene deletion using λ red
Genome extaraction
Lab Notebook
to view the files you will need Adobe Acrobat Reader or similar
Gene Deletion & Histidine Kinase
This is Rebecca's and Karen's lab notebook for gene deletion attempts and TaZ biobrick building.
Gate Construst
These are a few notebooks arranged together of all gate constructs. Lab work done by Tal, Rica, Ronen, Shira, Noa, Alex and Ittai.
Azobenzene
This is the lab notebook of all Azobenzene lab work done by Faris, our chemist. This notebook is all chemistry.
Safety
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