Team:Technion-Israel/Project
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Revision as of 09:43, 13 October 2014
The Project
"Success consists of going from failure to failure without loss of enthusiasm."--Winston Churchill
The Idea
How It works
Neetd lots of editing
Beta System
Beta System
Azobenzene
Histidine Kinase
Introfuction
Gene Deletion
Failed to delete ackA-pta genes
Experiments
Neetd lots of editing
Protocols
Here are all the protocols we used in the project
to view the files you will need Adobe Acrobat Reader or similar
Antibiotics Preparation
Chemical Trasformation
DNA Kit Plate Instructions
Z-Competent™ cells and Mix&Go
Gel/PCR Extraction
Gel Preparation
Gibson Assembly
Glycerol stock
LB, BA, SOB, SOC
Ligation
Presto Mini-prep
PCR
Phosphorylation and blunt ligation
Restriction enzyme
Electroporation Trasformation
Gene deletion using λ red
Genome extaraction
Chemical Trasformation
DNA Kit Plate Instructions
Z-Competent™ cells and Mix&Go
Gel/PCR Extraction
Gel Preparation
Gibson Assembly
Glycerol stock
LB, BA, SOB, SOC
Ligation
Presto Mini-prep
PCR
Phosphorylation and blunt ligation
Restriction enzyme
Electroporation Trasformation
Gene deletion using λ red
Genome extaraction
Lab Notebook
to view the files you will need Adobe Acrobat Reader or similar
Gene Deletion & Histidine Kinase
This is Rebecca's and Karen's lab notebook for gene deletion attempts and TaZ biobrick building.
Gate Construst
These are a few notebooks arranged together of all gate constructs. Lab work done by Tal, Rica, Ronen, Shira, Noa, Alex and Ittai.
Azobenzene
This is the lab notebook of all Azobenzene lab work done by Faris, our chemist. This notebook is all chemistry.
Safety
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