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| </p> | | </p> |
| <h2><br><u>Gate 1</u> </h2><h1>Built and Biobricked</h1> | | <h2><br><u>Gate 1</u> </h2><h1>Built and Biobricked</h1> |
- | <img src="images/Technion-Israel-gate1.png"><br> | + | <img src="https://static.igem.org/mediawiki/2014/f/fb/Technion-Israel-gate1.png"><br> |
| <h2><br>The goal of the first gate is to produce AHL molecules when a material of interest is present in the bacteria's environment.</h2> | | <h2><br>The goal of the first gate is to produce AHL molecules when a material of interest is present in the bacteria's environment.</h2> |
| <p>The construct includes a switchable promoter for the detection of the material of interest. In the example above the promoter is Ptet which is activated by tetR.<br> | | <p>The construct includes a switchable promoter for the detection of the material of interest. In the example above the promoter is Ptet which is activated by tetR.<br> |
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| Continue reading about gate 2 and 3 to find out</p></center> | | Continue reading about gate 2 and 3 to find out</p></center> |
| <center><h2><br><u>Gate 2</u> </h2><h1>Built and Biobricked</h1> | | <center><h2><br><u>Gate 2</u> </h2><h1>Built and Biobricked</h1> |
- | <img src="images/Technion-Israel-gate2.png"><br> | + | <img src="https://static.igem.org/mediawiki/2014/c/c5/Technion-Israel-gate2.png"><br> |
| <h2><br>The gate serves as a sensitivity tuner, allowing us to control on the timing of the measurement. When we want to start our measurement we just need to add IPTG to the bacteria solution.</h2> | | <h2><br>The gate serves as a sensitivity tuner, allowing us to control on the timing of the measurement. When we want to start our measurement we just need to add IPTG to the bacteria solution.</h2> |
| <p>The gate consists of the Plac promoter and the LuxR gene. When IPTG is added to a sample containing our bacteria, LuxR gene is transcribed. LuxR is a transcriptioninal activator that binds directly to the AHL molecules, so that a LuxR-AHL complex is formed. | | <p>The gate consists of the Plac promoter and the LuxR gene. When IPTG is added to a sample containing our bacteria, LuxR gene is transcribed. LuxR is a transcriptioninal activator that binds directly to the AHL molecules, so that a LuxR-AHL complex is formed. |
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| Read the next sections about gates 3 to find out</p></center> | | Read the next sections about gates 3 to find out</p></center> |
| <center><h2><br><u>Gate 3</u> </h2><h1>Failed to build</h1> | | <center><h2><br><u>Gate 3</u> </h2><h1>Failed to build</h1> |
- | <img src="images/Technion-Israel-gate3.png"><br> | + | <img src="https://static.igem.org/mediawiki/2014/d/d4/Technion-Israel-gate3.png"><br> |
| <h2><br>Gate 3 enables us to see if our material of interest is present in a random sample by producing GFP and amplifying the signal.</h2> | | <h2><br>Gate 3 enables us to see if our material of interest is present in a random sample by producing GFP and amplifying the signal.</h2> |
| <p>This construct is the final part of our alpha system. | | <p>This construct is the final part of our alpha system. |