Team:Tec-Monterrey/ITESM14 notebook data.html

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Tuesday, July 1st.

  • Practice: Minipreps, 13:00.
    • Eduardo Ramirez (1).
  • Practice: Ligations, 16:00.
    • Eduardo Ramirez (1).

Friday, July 2nd.

Friday, July 3rd.

Friday, July 4th.

  • Minipreps, 12:00.
    • Mercedes.
  • Practice: Ligations, 15:00.
    • Eduardo Ramirez (1).
  • Practice: Electrophoresis, 17:00.
    • Eduardo Ramirez (1)

Friday, July 5th.

Friday, July 6th.

Monday, July 7th.

  • Sendoffs of our teammates for a deserved vacation through the next 2 weeks.
  • Practice: Minipreps, 16:00.
    • Claudia.
  • Omar leaves for vacation.

Friday, July 8th.

Friday, July 9th.

Thursday, July 10th.

  • Practice: Transformation of calcium-competent cells, 11:00,
    • Eduardo Ramirez (1), Claudia.
  • Inoculation on LB-agar plates, 13:00.
    • Claudia.
  • Laboratory summer cleaning, 15:00.
    • Claudia.

Friday, July 11th.

Friday, July 12th.

Friday, July 13th.

Friday, July 14th.

Monday, July 15th.

  • Practice: taking DNA out of the iGEM plates, 13:00.
    • Claudia.
  • Transformation with calcium-competent cells, 13:30.
    • Claudia.
  • Inoculations, 15:00.
    • Claudia.

Tuesday, July 16th.

  • Minipreps, 12:00.
    • Eduardo Ramirez (1).
  • Ligations, 15:00.
    • Eduardo Ramirez (1).
  • Electrophoresis, 18:00.
  • Practice: DNA recovery from gel, 20:00.
    • Eduardo Ramirez (1)

Friday, July 14th.

Friday, July 14th.

Friday, July 19th.

  • Meeting with advisors to talk about gene problems and doubts, 17:00.

Friday, July 20th.

Friday, July 21st.

Monday, July 22nd.

  • Minipreps, 11:00.
    • Claudia.
  • Ligations, 15:00.
    • Claudia.
  • Electrophoresis, 19:00.
    • Claudia
  • Practice: DNA recovery from gel, 21:00.
    • Claudia.

Friday, July 23rd.

Friday, July 24th.

Friday, July 25th.

Friday, July 26th.

  • Meeting with advisors, 17:00.

 

Monday, July 29th.

  • Meeting to plan the semester’s activities, 14:00.
    • Schedule of people in the lab.
    • Ways to integrate other students.
    • Events to talk about iGEM.

Friday, July 27th.

Friday, July 28th.

Friday, July 29th.

Friday, July 30th.

Friday, July 31st.

Friday, August 8

  • Talk with new students about iGEM, 15:30.
    • Minerva, Mercedes, Cesar, Omar, Claudia, Eduardo Ramirez (1).
  • Session with advisors, 17:00.
    • Mercedes, Cesar, Omar, Claudia, Eduardo Ramirez (1).
  • Production of LB medium, 18:30.
    • Cesar.
  • Cleaning of glassware, 18:45.
    • Eduardo Ramirez (1).
  • Autoclave, 19:00.
    • Mercedes, Cesar, Eduardo Ramirez (1).
  • Grow H5-α culture, 21:00.
    • Cesar

Comments:  Most of the progress from today’s activities was not directly related to the laboratory. Keep in mind the session with the advisors next Friday (about the team rooster primarily) and the various events this month and the next with the general student population.

Saturday, August 9

  • Cleaning of glassware, 10:00.
  • Stock of the growth H5-α, 11:00.
  • Mini prep GFP, 15:00.
  • Calcium-competent of H5-α, 16:30.
  • Transformation of GFP (18C), GFP (18E), GFP (mini prep), RFP (O12), RFP (mini prep), (pLUX GFP). 9:40
    • Omar
  • Stock of the growth H5-α, 10:00.
    • Mercedes, Omar.
  • End of transformation (plaques), 23:23.
    • Omar, Mercedes.

Sunday, August 10

Monday, August 11

  • Selection of plate’s colonies to pass to Falcon tubes of 50 ml, 7:30
    • Mercedes.
  • Miniprep, 14:00
    • Omar
  • Digestions, 19:00.
    • Cesar
  • Electrophoresis, 21:00.
    • Cesar.

Tuesday, August 12

  • Making of electro-competent H5-α, 11:00.
    • Mercedes.

Wednesday, August 13

  • Mini-prep of GFP, 12:00.
    • Before incubation: Minerva.
    • After incubation: Omar, Claudia.
  • Transformation of the electro-competent cells with RFP, 17:00.
    • Eduardo Ramirez (1), Claudia, Omar.
  • Plating of the transformed cells, 21:00.
    • Eduardo Ramirez (1).
  • Electrophoresis of GFP mini-preps and digestions of GFP (Kn) and GFP (CAM), 21:10.
    • Cesar, Eduardo Cepeda.
      • Failed.

Saturday, August 16

  • Miniprep GFP, 12:00.
    • Mercedes.
  • Digestions, 15:30.
    • Mercedes.

Sunday, August 17

  • Preparation of TAE buffer, 18:00.
    • Mercedes.
  • Digestions, 20:00.
    • Mercedes.
  • Electrophoresis, 22:00.
    • Mercedes, Cesar, Claudia, Eduardo Ramirez (2), Omar.

Monday, August 18th.

  • Electrophoresis, 14:00.
    • Minerva.
  • Teaching of the newbies (Miniprep basics), 16:00.
    • Mercedes.
  • Autoclave of general materials, 18:00.
    • Everyone.

Tuesday, August 19

  • MiniPrep GFP, 12:30
    • Mercedes
  • Digestions, 18:00
    • Eduardo Cepeda
  • Electrophoresis, 21:00
    • Newbies (Alex, Dany).

Wednesday, August 20

  • iGEM talk with interested students, 16:30.
    • Special Guest: Ana Sifuentes
  • Teaching of how to make media to newbies, 18:00.
    • Claudia.

Thursday, August 21

  • Teaching of how to make an electrophoresis gel to the newbies, 18:00.
    • Claudia.

Friday, August 22

  • Teaching of how to make a transformation in calcium-competent cells, 17:00.
    • Claudia.
  • Purification of the Minipreps of GFP and the promoters D2 and D3, 18:00.
    • Eduardo Ramirez (2), Omar.
  • Electrophoresis of the past purifications, 22:00.
    • Mercedes.
  • Purification of the electrophoresis and ligations, 23:30.
    • Mercedes, Eduardo Cepeda.

Saturday, August 23

  • Making of electro-competent cells, 9:00.
    • Mercedes.
  • Visit to UANL to meet their iGEM team and to hear the iGEM HQ talk, 13:30.
    • Mercedes, Claudia, Eduardo Ramirez (1), Eduardo Cepeda.
  • Transformations of the electro-competent cells with D2, D3 and GFP, 18:00.
    • Claudia.
  • Inoculation of the ligation D2-GFP, 20:00.
    • Eduardo Cepeda.

Sunday, August 24

  • Miniprep of the ligation D2-GFP, 11:00.
    • Minerva.
  • Digestion of D2, D3, GFP and RFP, 16:00.
    • Minerva, Eduardo Cepeda.
  • Electrophoresis, 19:00.
    • Minerva, Eduardo Cepeda.
  • DNA purification of the gel, 20:15.
    • Mercedes.
  • Ligations D2-GFP, D3-GFP, 21:00.
    • Mercedes.

Monday, August 25

  • Miniprep D2-GFP, 13:00.
    • Eduardo Cepeda.
  • Digestion of D2, 16:00.
    • Eduardo Cepeda.
  • Inoculation practice with the newbies, 19:00.
    • Claudia.
  • Transformations with electro-competent cells, 21:30.
    • Cesar.
  • Preparation of plaques, 23:00.
    • Eduardo Cepeda.

Tuesday, August 26

  • ?

Wednesday, August 27

  • Inoculm of BL21 7:00.
    • Eduardo Cepeda.
  • Miniprep D2-GFP, D3-GFP, D2-RFP, D3-RFP, 13:30.
    • Eduardo Cepeda.
  • Digestions, 18:00.
    • Mercedes.
  • Electrophoresis, 20:00
    • Mercedes.
  • Preparation of calcium-competents BL21,
    • Claudia, Eduardo Ramirez.

Thursday, August 28

  • Electrophoresis, 22:00.
    • Newbies.
  • Preparation of calcium-competent cells TOP10, 22:00.
    • Eduardo Ramirez (2).

Friday, August 29

  • Preparation of chloramphenicol antibiotic, 10:00.
    • Mercedes.
  • Miniprep of D2-GFP and D3-GFP, 13:00.
    • Omar, Minerva.
  • Miniprep of D2 and D3 promoters, 15:00.
    • Omar.
  • Purification of the minipreps, 20:00.
    • Mercedes.
  • ?

Saturday, August 30

Sunday, August 31

  • Miniprep GFP Kn, RFP, 3 replicas of GFP Cam, 15:00.
    • Claudia, Eduardo Ramirez (2).
  • Digestions, 18:30.
    • Mercedes.
  • Electrophoresis, 22:40.
    • Omar.

Monday, September 1st.

  • Apoptosis kit arrived!, 14:00.
  • Accutase arrived!, 14:00.
  • Genes arrived!, 14:00.
  • Preparation of LB and LB-agar media, 13:30.
    • Eduardo Ramirez (1).
  • Preparation of the plaques for the transformation, 17:00.
    • Eduardo Cepeda.

Abbreviation

Construct

Gene

Resistance

LPP

C1

LPP Red Lambda

Amp

MSBB

C2

Red Lambda

Kn + Amp

H-GFP

C4

GFP

Amp

C-Apop

C5

Apoptine

Amp

H-SiRNA 1

C6

SiRNA 1

Amp

H-SiRNA 2

C7

SiRNA 2

Amp

H-SiRNA 3

C8

SiRNA 3

Amp

H-SiRNA-Apop

C9

SiRNA + Apoptine

Amp

BBa_1

Biobrick

  

Cam

BBa_2

Biobrick

  

Cam

BBa_3

Biobrick

Peptide + p3

Cam
  • HyperLadder 50bp arrived!, 17:10
  • HLA arrived!, 17:10:
  • Transformation of C1 through C7 in TOP10, 19:00.
    • Minerva.
  • Transformation of GFP and RFP in TOP10, 21:30.
    • Mercedes.

Tuesday, September 2

  • Inoculation of plates with transformed colonies from yesterday, 12:00.
    • Minerva.
  • Transformations of GFP Amp, RFP Cam, D1 Kn, D2 Cam, D3 Amp, 19:30.
    • Eduardo Ramirez (2).
  • Miniprep of the grown transformed cells from yesterday (C4, C5, C6, C7, D2, GFP, D1, BBa_1), 21:00.
    • Eduardo Cepeda.

Wednesday, September 3

  • Digestions of the last minipreps, 02:00.
    • Eduardo Cepeda.
  • Electrophoresis, 03:30.
    • Eduardo Cepeda.
  • Preparation of electro-competent TOP10 cells, 08:00.
    • Newbies: Alex, Danny, Chuy, Caro.
  • Preparation of stock cells (C4, C5, C6, C7, D2, GFP, D1, BBa_1), 18:30.
    • Mercedes.
  • Ligations of the C4, C5, C6, C7 with the pcb1c3, 20:30.
    • Eduardo Cepeda.
  • Inoculation of TOP10 for electro-competent cells tomorrow and D2 cells, 01:25.
    • Mercedes.

Thursday, September 4

Friday, September 5

  • Preparation of the tickets for the cake raffle (fundraising), 12:00.
    • Cinthya, Omar, Eduardo Ramirez (1), Eduardo Cepeda, Mercedes.
  • Miniprep, 14:00.
    • Mercedes and Eduardo Cepeda with special guest: Ana Cristina.
  • Talk with Professor Cardineau about the legislation proposal, 17:00.
    • Omar.
  • Preparations of electro-competente TOP10 cells, 17:30.
    • Eduardo Ramirez (2), Mercedes.
  • Digestions, 19:00.
    • Eduardo Cepeda.
  • Inoculation of LB-agar plaques, 21:00.
    • Cynthia.

Saturday, September 6

Sunday, September 7

Monday, September 8

Tuesday, September 9

  • Inoculation of PCB1c3 (with C4, C5, C7 separately), 13:30.
    • Eduardo Cepeda.

Wednesday, September 10

Thursday, September 11

  • Revision of (not grown yet) plates, 12:00.
    • Eduardo Cepeda.
  • Inoculaton of D2-GFP, 21:00.
    • Eduardo Cepeda.

Friday, September 12th

  • Cleaning of the electroporation cells for the electro-competent cells, 16:00.
    • Minerva.
  • Inoculation of cells that grew from the prior day, 18:00.
    • Cinthya.

Saturday, September 13

  • Transformation of the D2 and D3 variants for the interlab project, 00:10.
    • Eduardo Ramirez (2), Mercedes, Claudia, Omar.

Sunday, September 14th.

  • Miniprep, 17:00.
    • Mercedes, Eduardo Ramirez (2)
  • Sterilization of Falcon tubes, LB broth and plastic pearls, 18:30.
    • Omar
  • Digestions, 21:00.
    • Mercedes.
  • Inoculation of the transformed cells with D2 and D3 variants, 22:00.
    • Omar.

Monday, September 15

  • Miniprep of yesterday's inoculations of D2 and D3 variants, 10:00.
    • Mercedes.
  • Digestions, 17:30.
    • Eduardo Ramirez (2).
  • Inoculation of TOP10 and XL-Blue (Tet), 22:50.
    • Mercedes.

Tuesday, September 16th.

  • Transformations of the M13 bacteriophage into the XL-Blue, 10:00.
    • Mercedes.

Wednesday, September 17

  • Miniprep 01:15.
    • Minerva, Eduardo Cepeda.
  • Digestions (C4, C5, C6, C7 and , 02:53.
    • Eduardo Cepeda.
  • PBS buffer, 12:30.
    • Omar.
  • Recovery of DNA from Gel, 13:30.
    • Eduardo Cepeda.

Thursday, September 18

  • Transformations of C8, C9 and ligation in TOP10 electro-competent, 00:40.
    • Cinthya, Omar.
  • Electrophoresis, 01:30.
    • Mercedes, Eduardo Ramirez (1).
  • Plates from the transformations, 01:50.
    • Omar.
  • Miniprep of PET, 12:30.
    • Eduardo Cepeda.
  • Transformation replica of the same ligation as before, 15:30.
    • Omar.
  • Preparation of LB broth, 17:00.
    • All the newbies (Caro, Jesus, Alex, Dany).
  • Digestion of PET, 19:30.
    • Claudia.

Friday, September 19

  • Ligation of PET with all the constructs, 01:19.
    • Eduardo Cepeda.
  • Electrophoresis, 03:30.
    • Eduardo Cepeda.
  • Miniprep, 8:30.
    • Mercedes.
  • Digestions, 13:00.
    • Mercedes.
  • Electrophoresis, 15:30.
    • Mercedes.
  • Quantification of the fluorescence for the interlab project, 16:30.
    • Mercedes, Eduardo Ramirez (2).
  • Group photo taken, 18:00.
    • Everyone except Mercedes, Eduardo Cepeda and Eduardo Ramirez.
  • Group video filmed, 18:30.
    • Everyone.
  • Transformation of TOP10 cells with PET + (C4, C5, C6, C7), 20:30.
    • Cinthya, Eduardo Cepeda.
  • Transformation of TOP10 cells with various repetitions of D2 and D3 (with GFP or RFP), 21:00.
    • Mercedes, Eduardo Ramirez (2).
  • Inoculation in Falcon tubes with the ligation of C5 and various others, 22:00.
    • Omar.
  • Inoculation in plates of the previous transformed TOP10 cells with PET, 23:00.
    • Cinthya.

Saturday, September 20

  • Transformation in electro-competent cells, 01:20.
    • Mercedes, Eduardo Ramirez.
  • Minipreps, 18:00.
    • Mercedes, Omar.
  • Digestions, 22:00.

Sunday, September 21st.

  • Electrophoresis, 00:00.
    • Mercedes.
  • Inoculations for minipreps, 01:00.
    • Mercedes.
  • Minipreps,
    • Eduardo Cepeda.

Monday, September 22

  • Miniprep, 08:00.
    • Mercedes.
  • Revision of the cell stocks, contamination detected, 09:00.
    • Mercedes.
  • Inoculations of C8 and C9, 22:00.
    • Eduardo Cepeda.

Tuesday, September 23

  • Preparation of electo-competent cells DH5a, 03:00.
    • Minerva.
  • Minipreps for the interlab, 06:00
    • Mercedes, Minerva.
  • Transformations for the interlab, 07:30.
    • Mercedes, Minerva.
  • Digestions for the interlab, 12:30.
    • Mercedes.
  • Miniprep, 13:30.
    • Eduardo Cepeda.
  • Electrophoresis for interlab, 19:00.
    • Eduardo Ramirez (2)
  • Electrophoresis for the project (C8, C9), 21:00.
    • Eduardo Ramirez (2)

Wednesday, September 24

  • Purification of the electrophoresis gel, 01:20.
    • Mercedes.
  • Digestions for the interlab, 01:20.
    • Eduardo Cepeda.
  • Ligations, 02:00.
    • Eduardo Cepeda.
  • Electrophoresis for the interlab, 02:40.
    • Mercedes.
  • Inoculations for the interlab, 11:00.
    • Omar, Mercedes.

Thursday, September 25

  • Inoculation of XL-Blue, 22:00.
    • Mercedes.
  • Infection of the XL-Blue with bacteriophages, 23:00.
    • Mercedes.

Friday, September 26

  • Miniprep of the bacteriophage-infected cells, 03:00.
    • Mercedes.
  • Preparation of the cells for quantification of fluorescence and OD for the interlab project, from 8:00 to 24:00.
    • Mercedes.
  • Purification of the miniprep. 18:00.
    • Eduardo Ramirez (1), Claudia.
  • Transformations of C1, C2, Bba_1, Bba_2, and ligations, 19:00.
    • Cinthya, Omar.
  • Plating of the transformations, 22:00.
    • Cinthya.

Saturday, September 27

  • Inoculation of C1, C2, Bba_1, Bba _2 and ligations, 20:00.
    • Omar
  • Sterilization of material, 22:00.
    • Omar.

Sunday, September 28

  • Preparation of electro-competent cells, 01:00.
    • Eduardo Cepeda.
  • Ligations, 03:00.
    • Eduardo Cepeda.
  • Electrophoresis, 12:00.
    • Eduardo Cepeda.
  • Minipreps of the inoculations from Saturday, 13:00.
    • Claudia, Eduardo Ramirez (1).
  • Recovery of DNA from gel, 15:00.
    • Claudia.

Monday, September 29

Tuesday, September 30

Retrieved from "http://2014.igem.org/Team:Tec-Monterrey/ITESM14_notebook_data.html"