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| | <ul> | | <ul> |
| | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Team"><font size="3">Members</font></a></li> | | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Team"><font size="3">Members</font></a></li> |
| | + | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Achievements"><font size="3">Achievements</font></a></li> |
| | <li><a href="https://igem.org/Team.cgi?id=1473" target="_blank"><font size="3">Official Team Profile</font></a></li> | | <li><a href="https://igem.org/Team.cgi?id=1473" target="_blank"><font size="3">Official Team Profile</font></a></li> |
| | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Contact"><font size="3">Contact</font></a></li> | | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Contact"><font size="3">Contact</font></a></li> |
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| | onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B> | | onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B> |
| | <a href="#topic1" | | <a href="#topic1" |
| - | style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Topic 1</font></a> </td> | + | style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Protagonist</font></a> </td> |
| | </tr> | | </tr> |
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| | onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B> | | onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B> |
| | <a href="#topic2" | | <a href="#topic2" |
| - | style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Topic 2</font></a> </td> | + | style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Super Cast</font></a> </td> |
| | </tr> | | </tr> |
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| | <td style="border:0px solid black;" align="center" width="33%" height ="35px" | | <td style="border:0px solid black;" align="center" width="33%" height ="35px" |
| | onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B> | | onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B> |
| - | <a href="#topic3" | + | <a href="#Story" |
| - | style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Topic 3</font></a> </td> | + | style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">The Story</font></a> </td> |
| | </tr> | | </tr> |
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| | <td style="border:0px solid black;" align="center" width="33%" height ="35px" | | <td style="border:0px solid black;" align="center" width="33%" height ="35px" |
| | onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B> | | onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B> |
| - | <a href="#references" | + | <a href="#Program" |
| - | style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">References</font></a> </td> | + | style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Program</font></a> </td> |
| | </tr> | | </tr> |
| | + | |
| | <tr> | | <tr> |
| | <td align="right" height ="35px"><img src="https://static.igem.org/mediawiki/2014/b/b8/TCU-Phage.png" width="30%"/></td> | | <td align="right" height ="35px"><img src="https://static.igem.org/mediawiki/2014/b/b8/TCU-Phage.png" width="30%"/></td> |
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| | <tr ><td coslpan="3" height="35px"></td></tr> | | <tr ><td coslpan="3" height="35px"></td></tr> |
| | | | |
| - | <tr><td colspan="3" style="background-color:#FFF2B5" height="20px"> <font face="Trebuchet MS" size="7" color="#A66B38" id="abstract">Abstract</font> </td> </tr> | + | <tr><td colspan="3" style="background-color:#FFF2B5" height="20px"> <font face="Trebuchet MS" size="6" color="#A66B38" id="abstract">Abstract</font> </td> </tr> |
| | <tr> | | <tr> |
| | <td valign="top" height="10px"> </td> | | <td valign="top" height="10px"> </td> |
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| | <table width="975px" align="center"> | | <table width="975px" align="center"> |
| | | | |
| - | <tr> <td> <h3 id="topic1">Topic 1</h3> </td> </tr> | + | <tr> <td style="background-color:#FFF2B5" height="20px"> <h3 id="topic1"><font face="Trebuchet MS" size="6" color="#A66B38">Protagonist</font></h3> </td> </tr> |
| | | | |
| | <tr> | | <tr> |
| | <!-- first column --> | | <!-- first column --> |
| - | <td width="30%"> | + | <td width="50%" colspan="3"> |
| - | <p> </p> | + | <font size="4" face="Verdana" color="#333"><p> Trogene horse is a lovely wooden horse, but he carries a super weapon inside, and this weapon can help greek soldiers defeat Troy city.</p ></font> |
| | </td> | | </td> |
| | | | |
| - | <td width="5%"></td>
| + | |
| | | | |
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| | <!-- second column --> | | <!-- second column --> |
| - | <td width="30%">
| |
| - | <p> </p>
| |
| - | </td>
| |
| | | | |
| - | <td width="5%"></td> | + | |
| | + | <td width="30"></td> |
| | | | |
| | <!-- third column --> | | <!-- third column --> |
| - | <td width="30%"> | + | <td align="center"> |
| - | <img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="300px"> | + | <img src="https://static.igem.org/mediawiki/2014/c/c2/TCU-Logo-up.png" width="490" height="381"> |
| | </td> | | </td> |
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| | <table width="975px" align="center"> | | <table width="975px" align="center"> |
| | | | |
| - | <tr> <td> <h3 id="topic2">Topic 2</h3> </td> </tr> | + | <tr> <td style="background-color:#FFF2B5" height="20px"> <h3 id="topic2"><font face="Trebuchet MS" size="6" color="#A66B38" id="abstract">Super Cast</font></h3> </td> </tr> |
| | | | |
| | <tr> | | <tr> |
| | <!-- first column --> | | <!-- first column --> |
| - | <td width="30%"> | + | <td align="center"> |
| - | <img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="300px"> | + | <img src="https://static.igem.org/mediawiki/2014/c/cc/TCU-Anti.png" width="200px"> |
| | </td> | | </td> |
| | | | |
| - | <td width="5%"></td> | + | <td width="10"> </td> |
| | + | <td><font size="5" face="Verdana" color="#0092C7"><p>Antibiotics：</p></font><font size="4" face="Verdana" color="#333"> |
| | + | <p>A group of greek soldiers who is eager to conquest troy city. </p> |
| | + | </font></td> |
| | + | <td width="30"> </td> |
| | | | |
| | | | |
| | <!-- second column --> | | <!-- second column --> |
| - | <td width="30%"> | + | <td align="center"> |
| - | <img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="300px"> | + | <img src="https://static.igem.org/mediawiki/2014/d/d3/TCU_Igem_cell.png" width="120"> |
| | </td> | | </td> |
| - | <td width="5%"></td> | + | <td width="10"> </td> |
| | | | |
| | | | |
| | <!-- third column --> | | <!-- third column --> |
| | <td width="30%"> | | <td width="30%"> |
| - | <p> </p> | + | <font size="5" face="Verdana" color="#0092C7"><p> Bacterium：</p ></font><font size="4" face="Verdana" color="#333"> |
| | + | <p>Troy citizens who lives happily under the protection of troy wall. </p> |
| | + | </font> |
| | </td> | | </td> |
| | </tr> | | </tr> |
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| | <table width="975px" align="center"> | | <table width="975px" align="center"> |
| | | | |
| - | <tr> <td> <h3 id="topic3">Topic 3</h3> </td> </tr> | + | <tr> <td> <h3 id="topic3"> </h3> </td> </tr> |
| | | | |
| | <tr> | | <tr> |
| | <!-- first column --> | | <!-- first column --> |
| | | | |
| - | <td width="65%"> | + | <td align="center" width="30%"> |
| - | <p> </p> | + | <p><img src="https://static.igem.org/mediawiki/2014/7/7e/TCU_WALL.png" width="300" height="300" /></p> |
| | </td> | | </td> |
| | | | |
| | | | |
| - | <td width="5%"></td> | + | <td width="30"></td> |
| | | | |
| | | | |
| | <!-- second column --> | | <!-- second column --> |
| - | <td width="30%"> | + | <td><font size="5" face="Verdana" color="#0092C7"><p>Troy Wall: </p></font><font size="4" face="Verdana" color="#333"> |
| - | <img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="200px">
| + | <p>A great wall of Troy city, it is the nightmare of greek soldiers. </p> |
| - | </td> | + | </font></td> |
| | | | |
| | | | |
| | + | </tr> |
| | + | <tr> |
| | + | <td align="center" height="30"> </td> |
| | + | <td></td> |
| | + | <td> </td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td align="center"><img src="https://static.igem.org/mediawiki/2014/1/11/TCU_GODDESS.png" width="300" height="300" /></td> |
| | + | <td></td> |
| | + | <td><font size="5" face="Verdana" color="#0092C7"><p>TCU-Taiwan：</p></font><font size="4" face="Verdana" color="#333"> |
| | + | <p>Greek soldiers’ goddess who designed secret agents can break troy wall and hide them inside the Trogene Horse. With this, Trogene Horse become a powerful weapon of greek soldiers.</p> |
| | + | </font></td> |
| | </tr> | | </tr> |
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| | </table> | | </table> |
| | <!-- end of 2 column content --> | | <!-- end of 2 column content --> |
| - |
| |
| - | <!-- references -->
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| - |
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| - | <table width="975px" align="center">
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| - | <tr ><td height="35px"></td></tr>
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| - |
| |
| - | <tr><td> <h3 id="references">References</h3>
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| - | <ul>
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| - | <li></li>
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| - | </ul>
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| - | </td>
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| - | </tr>
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| - |
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| - | </tr></table>
| |
| | <table width="975" border="0" cellspacing="0" cellpadding="0" align="center"> | | <table width="975" border="0" cellspacing="0" cellpadding="0" align="center"> |
| | <tr> | | <tr> |
| - | <td style="background-color:#FFF2B5" height="20px"><font face="Trebuchet MS" size="7" color="#A66B38" id="abstract">Program</font></td> | + | <td height="30"> </td> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <td height="15"> </td> | + | <td style="background-color:#FFF2B5" height="20px" align="center"><h3 id="Story"><font face="Trebuchet MS" size="6" color="#A66B38">The Story</font></h3> </td> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <td> | + | <td height="15"> </td> |
| - | <table border="0" cellspacing="0" cellpadding="0" align="center">
| + | |
| - | <tr>
| + | |
| - | <td align="center" width="15"> </td>
| + | |
| - | <td align="center" width="250" height="250"><img src="https://static.igem.org/mediawiki/2014/e/e5/TCU-Part-logo.png" alt="Parts" width="128"onmouseover="this.width=this.width*1.2;Partsbox.color='#90B849'" onmouseout="this.width=this.width/1.2;Partsbox.color='#333'"/></td>
| + | |
| - | <td width="20"> </td>
| + | |
| - | <td align="center" width="250"><img src="https://static.igem.org/mediawiki/2014/4/4d/TCU_Blueprint-128.png" width="128" height="128" alt="System Design"/></td>
| + | |
| - | <td width="20"> </td>
| + | |
| - | <td align="center" width="250"><img src="https://static.igem.org/mediawiki/2014/2/24/TCU_Microscope-128.png" alt="M13 Phage" width="128" height="128" /></td>
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| - | <td align="center" width="15"> </td>
| + | |
| - | </tr>
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| - | <tr>
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| - | <td align="center"> </td>
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| - | <td align="center" height="10"> </td>
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| - | <td> </td>
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| - | <td> </td>
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| - | <td align="center"> </td>
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| - | <td align="center"><font size="6" face="Verdana" color="#333" id="Partsbox">Parts</font></td>
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| - | <td> </td>
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| - | <td align="center"><font size="6" face="Verdana" color="#333" id="Systembox">System Design</font></td>
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| - | <td> </td>
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| - | <td align="center"><font size="6" face="Verdana" color="#333" id="M13box">M13 Phage</font></td>
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| - | <td align="center"> </td>
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| - | </tr>
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| - | <tr>
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| - | <td align="center"> </td>
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| - | <td align="center" width="250" height="250"><img src="https://static.igem.org/mediawiki/2014/4/43/TCU_Gear-128.png" alt="CRISPR" width="128" height="128" /></td>
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| - | <td> </td>
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| - | <td align="center"><img src="https://static.igem.org/mediawiki/2014/4/4a/TCU_1413400508_Briefcase.png" alt="Future Works" width="128" height="128" /></td>
| + | |
| - | <td> </td>
| + | |
| - | <td align="center"><img src="https://static.igem.org/mediawiki/2014/d/dd/TCU-Human-logo.png" alt="Human Pratics" width="128" height="128" /></td>
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| - | <td align="center"> </td>
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| - | </tr>
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| - | <td align="center"> </td>
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| - | <td align="center"><font size="6" face="Verdana" color="#333" id="CRISPRbox">CRISPR</font></td>
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| - | <td> </td>
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| - | <td align="center"><font size="6" face="Verdana" color="#333" id="Futurebox">Future Work</font></td>
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| - | <td> </td>
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| - | <td align="center"><font size="6" face="Verdana" color="#333" id="Humanbox">Human Pratics</font></td>
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| - | <td align="center"> </td>
| + | |
| - | </tr>
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| - | </table></td>
| + | |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <td height="30"> </td> | + | <td align="center"> |
| - | </tr>
| + | <video width="640" height="360" controls> |
| - | </table>
| + | <source src="https://static.igem.org/mediawiki/2014/9/95/TCU-Taiwan_An_Introduction_of_Trogene_Horse_%28720p%29.mov" type="video/mp4"> |
| - | | + | <iframe width="853" height="480" src="//www.youtube.com/embed/jMb2o3Bwiq8?showinfo=0" frameborder="0" allowfullscreen></iframe> |
| - | <table width="100%" border="0" cellspacing="0" cellpadding="0">
| + | <object> |
| - | <tr>
| + | Your browser does not support the HTML5 video tag! |
| - | <td><img src="https://static.igem.org/mediawiki/2014/c/c1/TCU-Line.png" width="100%"></td>
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/TCU-Taiwan_An_Introduction_of_Trogene_Horse_%28720p%29.mov"><br>Download the video.</a> |
| - | </tr>
| + | </object> |
| - | </table>
| + | </video> |
| - | <table width="100%" border="0" align="center" style="background-color:#F2DE79" height="150px">
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| - | <tr align="center" bgcolor="#F2DE79" height="150px" style="width:150px">
| + | |
| - | <td width="15%" align="center"><div id="gotop">^</div>
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| - | <a href="https://2014.igem.org/Team:TCU_Taiwan/Contact" title="Contact Us"><img src="https://static.igem.org/mediawiki/2014/8/84/TCU-Contact-bot.png" width="80" onMouseMove="this.src=Image0.src;" onMouseOut="this.src='https://static.igem.org/mediawiki/2014/8/84/TCU-Contact-bot.png'"></a>
| + | |
| - | <br><br>
| + | |
| - | <a href ="mailto: tcutaiwan@gmail.com" target="_blank" title="Gmail"><img src="https://static.igem.org/mediawiki/2014/ c/ c4/TCU% E3% 84%A6Gmail. png" width="30">< /a> | + | |
| - | <a href="https://www.facebook.com/TCUiGEM?ref=ts&fref=ts" target="_blank" title="Facebook"><img src="https://static.igem.org/mediawiki/2014/b/b9/TCU-Facebook.png" width="30"></a> <a href="https://www.youtube.com/channel/UCr40ZmaFNJGx7tUufCXK_OQ" target="_blank" title="YouTube"><img src="https://static.igem.org/mediawiki/2014/2/29/TCU_Youtube.png" width="30"></a> <a href="http://tcuigem.wordpress.com/" target="_blank" title="Wordpress"><img src="https://static.igem.org/mediawiki/2014/f/f0/TCU-Wordpress.png" width="30"></a>
| + | |
| | </td> | | </td> |
| - | <td width="0.5px" align="center" bgcolor="#A66B38"> </td>
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| - | <td>
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| - | <table width="70%" border="0" cellspacing="0" cellpadding="0" align="center" style="background-color:#F2DE79">
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| - | <tr align="center">
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| - | <td><font size="2" face="Verdana" color="#F2DE79" id="Members">Team Members</font></td>
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| - | <td><font size="2" face="Verdana" color="#F2DE79" id="Project">Project</font></td>
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| - | <td><font size="2" face="Verdana" color="#F2DE79" id="Parts">Parts</font></td>
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| - | <td><font size="2" face="Verdana" color="#F2DE79" id="Pratics">Human Pratics</font></td>
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| - | <td><font size="2" face="Verdana" color="#F2DE79" id="Modeling">Modeling</font></td>
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| - | <td><font size="2" face="Verdana" color="#F2DE79" id="Safety">Safety</font></td>
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| - | <td><font size="2" face="Verdana" color="#F2DE79" id="Notebook">Notebook</font></td>
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| - | <td><font size="2" face="Verdana" color="#F2DE79" id="Attributions">Attributions</font></td>
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| - | </tr>
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| - | <tr>
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| - | <td><a href="https://2014.igem.org/Team:TCU_Taiwan/Team" title="Team Members"><img src="https://static.igem.org/mediawiki/2014/1/1a/TCU-Team-logo.png" width="100"onMouseOver="this.width=this.width*1.1;Members.color='#333'" onMouseOut="this.width=this.width/1.1;Members.color='#F2DE79'"></a></td>
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| - | <td><a href="https://2014.igem.org/Team:TCU_Taiwan/Project" title="Project"><img src="https://static.igem.org/mediawiki/2014/f/f4/TCU-Project-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Project.color='#333'" onMouseOut="this.width=this.width/1.1;Project.color='#F2DE79'"></a></td>
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| - | <td><a href="https://2014.igem.org/Team:TCU_Taiwan/Parts" title="Parts"><img src="https://static.igem.org/mediawiki/2014/e/e5/TCU-Part-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Parts.color='#333'" onMouseOut="this.width=this.width/1.1;Parts.color='#F2DE79'"></a></td>
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| - | <td><a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics" title="Human Pratics"><img src="https://static.igem.org/mediawiki/2014/d/dd/TCU-Human-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Pratics.color='#333'" onMouseOut="this.width=this.width/1.1;Pratics.color='#F2DE79'"></a></td>
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| - | <td><a href="https://2014.igem.org/Team:TCU_Taiwan/Modeling" title="Modeling"><img src="https://static.igem.org/mediawiki/2014/3/3f/TCU-Modeling-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Modeling.color='#333'" onMouseOut="this.width=this.width/1.1;Modeling.color='#F2DE79'"></a></td>
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| - | <td style="background-color:#FFF2B5" height="20px"><font face="Trebuchet MS" size="7" color="#A66B38">Trogene Horse</font></td>
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| - | <tr><td colspan="3" style="background-color:#FFF2B5" height="20px"> <font face="Trebuchet MS" size="7" color="#A66B38" id="abstract">Abstract</font> </td> </tr>
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| - | <font size="3" face="Verdana" color="#333"><p>To access this, we use a special <a href="http://en.wikipedia.org/wiki/Phagemid" target="_blank">phagemid</a> for phage display. The phagemid contains a additional phage replication origin. When helper phage (a phage whose own ori is knock-out) infection take place, phagemid can be packaged as genome for progeny. Since we put an <a href="http://en.wikipedia.org/wiki/Antibiotic_resistance" target="_blank">antibiotic</a> resistance gene targeting <a href="http://en.wikipedia.org/wiki/CRISPR" target="_blank">CRISPR</a> system on our phagemid, this phage display can spread our CRISPR among bacterium nearby and remove their resistance efficiently.</p></font>
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| - | <font size="3" face="Verdana" color="#333"><p>Trogene Horse is a new concept combining phage therapy with CRISPR, and this combination can solve both safety concern(phage therapy) and efficiency problem(CRISPR). We believe our project can deal with bacterial resistance problem on agriculture, and hope it can access on medicine one day.</p></font></td>
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| - | <tr><td> <h3 id="references">References</h3>
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| - | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Modeling"><font size="5">Modeling</font></a>
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| - | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Safety"><font size="5">Safety</font></a></li>
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| - | </td>
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| - | <!-- this table contains the navigation bar --><!--navigation menu -->
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| - | <td align="center" colspan="3">
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| - | <td> </td>
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| - | <a href="#abstract"
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| - | <td align="right" height ="35px"><img src="https://static.igem.org/mediawiki/2014/b/b8/TCU-Phage.png" width="30%"/></td>
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| - | </td>
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| - | </tr>
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| - | </table>
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| - |
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| - | <!-- Abstract Section-->
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| - | <font size="3" face="Verdana" color="#333"><p>To access this, we use a special <a href="http://en.wikipedia.org/wiki/Phagemid" target="_blank">phagemid</a> for phage display. The phagemid contains a additional phage replication origin. When helper phage (a phage whose own ori is knock-out) infection take place, phagemid can be packaged as genome for progeny. Since we put an <a href="http://en.wikipedia.org/wiki/Antibiotic_resistance" target="_blank">antibiotic</a> resistance gene targeting <a href="http://en.wikipedia.org/wiki/CRISPR" target="_blank">CRISPR</a> system on our phagemid, this phage display can spread our CRISPR among bacterium nearby and remove their resistance efficiently.</p></font>
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| - | <font size="3" face="Verdana" color="#333"><p>Trogene Horse is a new concept combining phage therapy with CRISPR, and this combination can solve both safety concern(phage therapy) and efficiency problem(CRISPR). We believe our project can deal with bacterial resistance problem on agriculture, and hope it can access on medicine one day.</p></font></td>
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