Team:Stony Brook/Team

From 2014.igem.org

(Difference between revisions)
Line 1: Line 1:
-
<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN" "http://www.w3.org/TR/html4/loose.dtd">
+
{{CSS/Main}}
 +
 
<html>
<html>
-
<head>
 
-
<title>project page.gif</title>
 
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
 
-
<style type="text/css">
 
-
figure{
 
-
  display:table;
 
-
}
 
-
figcaption{
 
-
  display:table-caption;
 
-
  caption-side:bottom;
 
-
  font-size: x-small;
 
-
  text-align:center;
 
-
}
 
-
#abstract{
 
-
position: absolute;
 
-
top: 305px;
 
-
width:450px;
 
-
left:50%;
 
-
margin-left:-490px;
 
-
font-family:"Trebuchet MS", Arial, Helvetica, sans-serif;
 
-
font-size: small;
 
-
}
 
-
#slideshow{
+
<!--main content -->
-
position: absolute;
+
<table width="70%" align="center">
-
top: 305px;
+
-
height:250px;
+
-
right:50%;
+
-
margin-right:-450px;;
+
-
}
+
-
#content{
 
-
position:absolute;
 
-
top: 550px;
 
-
width: 1200px;
 
-
left:50%;
 
-
margin-left:-590px;
 
-
}
 
-
#yellow_header{
 
-
position: absolute;
 
-
width: 100%;
 
-
top: 0%;
 
-
height: 18px;
 
-
background-color: #FDCA1C;
 
-
padding: 0;
 
-
left:0;
 
-
font-size: x-small;
 
-
font-weight: bold;
 
-
}
 
-
#yellow_header a{
 
-
text-decoration: none;
 
-
height: 18px;
 
-
line-height: 18px;
 
-
padding: 20px;
 
-
font-family: "Trebuchet MS", Arial, Helvetica, sans-serif;
 
-
color: #000;
 
-
font-size: x-small;
 
-
font-weight: normal;
 
-
}
 
-
#yellow_header a:hover{
+
<!--welcome box -->
-
color:#FFFFFF;
+
<tr>
-
}
+
<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
-
 
+
<h1 >WELCOME TO iGEM 2014! </h1>
-
#blue_header{
+
<p>Your team has been approved and you are ready to start the iGEM season!
-
position: absolute;
+
<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
-
height: 170px;
+
-
width: 100%;
+
-
background-color: #234678;
+
-
padding: 0px;
+
-
left: 0;
+
-
top: 18px;
+
-
}
+
-
+
-
#blue_header a img{
+
-
border: none;
+
-
}
+
-
#blue_header a:hover img{
+
-
opacity: 0.8;
+
-
filter:alpha(opacity=80);
+
-
}
+
-
 
+
-
+
-
#apis-biotics{
+
-
position: absolute;
+
-
top: 17%;
+
-
right: 50%;
+
-
margin-right: -112px;
+
-
}
+
-
 
+
-
#yellow_navigation{
+
-
width: 53px;
+
-
float: left;
+
-
padding: 7px;
+
-
color: #FFF;
+
-
font-family:"Trebuchet MS", Arial, Helvetica, sans-serif;
+
-
text-align: center;
+
-
font-size:x-small;
+
-
font-weight:bold;
+
-
 
+
-
}
+
-
 
+
-
#yellow_navigation p{
+
-
display: none;
+
-
}
+
-
 
+
-
#yellow_navigation a:hover + p{
+
-
display: block;
+
-
}
+
-
 
+
-
#yellow_navigation a img{
+
-
border: none;
+
-
}
+
-
 
+
-
#yellow_navigation_container{
+
-
width: 405px;
+
-
height: 86px;
+
-
position: absolute;
+
-
top: 38%;
+
-
right: 50%;
+
-
margin-right: -203px;
+
-
}
+
-
 
+
-
#project {
+
-
position: absolute;
+
-
height: 42px;
+
-
width: 1000px;
+
-
top: 240px;
+
-
left:50%;
+
-
margin-left: -500px;
+
-
padding:0;
+
-
background-color: #FDCA1C;
+
-
}
+
-
 
+
-
#project p{
+
-
position:absolute;
+
-
padding-left:20px;
+
-
left: 6%;
+
-
font-family:Arial, Helvetica, sans-serif;
+
-
font-weight: bold;
+
-
}
+
-
 
+
-
.tabs {
+
-
    position: relative;
+
-
margin: 40px auto;
+
-
width: 1200px; /**width tabs section**/
+
-
}
+
-
 
+
-
.tabs input {
+
-
position: absolute;
+
-
z-index: 1000;
+
-
width: 235px; /**click region**/
+
-
height: 36px;
+
-
left: 0px;
+
-
top: 0px;
+
-
opacity: 0;
+
-
-ms-filter:"progid:DXImageTransform.Microsoft.Alpha(Opacity=0)";
+
-
filter: alpha(opacity=0);
+
-
cursor: pointer;
+
-
}
+
-
.tabs input#tab-2{
+
-
left: 242px; /** position**/
+
-
}
+
-
.tabs input#tab-3{
+
-
left: 478px;
+
-
}
+
-
.tabs input#tab-4{
+
-
left: 723px;
+
-
}
+
-
 
+
-
.tabs label {
+
-
background: #4F86D9;
+
-
font-size: small;
+
-
line-height: 40px;
+
-
height: 40px;
+
-
position: relative;
+
-
float: left;
+
-
display: block;
+
-
width: 200px; /** width of tab **/
+
-
color: #000000;
+
-
font-family:"Trebuchet MS", Arial, Helvetica, sans-serif;
+
-
letter-spacing: 1px;
+
-
font-weight: bold;
+
-
text-align: center;
+
-
text-shadow: 1px 1px 1px rgba(255,255,255,0.3);
+
-
border-radius: 3px 3px 0 0;
+
-
box-shadow: 2px 0 2px rgba(0,0,0,0.1), -2px 0 2px rgba(0,0,0,0.1);
+
-
padding-top: 0;
+
-
padding-right: 20px;
+
-
padding-bottom: 0;
+
-
padding-left: 20px;
+
-
}
+
-
 
+
-
.tabs label:after {
+
-
    content: '';
+
-
position: absolute;
+
-
bottom: -2px;
+
-
left: 0;
+
-
width: 100%;
+
-
height: 2px;
+
-
display: block;
+
-
}
+
-
 
+
-
.tabs input:hover + label {
+
-
background: #E0ECF8;
+
-
}
+
-
 
+
-
.tabs label:first-of-type {
+
-
    z-index: 5;
+
-
    box-shadow: 2px 0 2px rgba(0,0,0,0.1);
+
-
}
+
-
 
+
-
.tab-label-2 {
+
-
    z-index: 4;
+
-
}
+
-
 
+
-
.tab-label-3 {
+
-
    z-index: 3;
+
-
}
+
-
 
+
-
.tab-label-4 {
+
-
    z-index: 2;
+
-
}
+
-
 
+
-
 
+
-
.tabs input:checked + label {
+
-
    background: #E0ECF8;
+
-
z-index: 6;
+
-
}
+
-
 
+
-
.clear-shadow {
+
-
clear: both;
+
-
}
+
-
 
+
-
.content {
+
-
background: #E0ECF8;
+
-
position: relative;
+
-
width: 1200px;
+
-
//**height: 2000px;**//
+
-
z-index: 5;
+
-
box-shadow: 0 -2px 3px -2px rgba(0,0,0,0.2), 0 2px 2px rgba(0,0,0,0.1);
+
-
border-radius: 0 3px 3px 3px;
+
-
font-family: "Trebuchet MS", Arial, Helvetica, sans-serif;
+
-
font-size:small;
+
-
}
+
-
 
+
-
.content div {
+
-
    position: absolute;
+
-
top: 10;
+
-
left: 0;
+
-
padding: 10px 40px;
+
-
z-index: 1;
+
-
    opacity: 0;
+
-
 
+
-
    -webkit-transition: opacity linear 0.1s;
+
-
    -moz-transition: opacity linear 0.1s;
+
-
    -o-transition: opacity linear 0.1s;
+
-
    -ms-transition: opacity linear 0.1s;
+
-
    transition: opacity linear 0.1s;
+
-
}
+
-
 
+
-
.tabs input.tab-selector-1:checked ~ .content .content-1,
+
-
.tabs input.tab-selector-2:checked ~ .content .content-2,
+
-
.tabs input.tab-selector-3:checked ~ .content .content-3,
+
-
.tabs input.tab-selector-4:checked ~ .content .content-4 {
+
-
z-index: 100;
+
-
    -ms-filter:"progid:DXImageTransform.Microsoft.Alpha(Opacity=100)";
+
-
    filter: alpha(opacity=100);
+
-
    opacity: 1;
+
-
 
+
-
    -webkit-transition: opacity ease-out 0.2s 0.1s;
+
-
    -moz-transition: opacity ease-out 0.2s 0.1s;
+
-
    -o-transition: opacity ease-out 0.2s 0.1s;
+
-
    -ms-transition: opacity ease-out 0.2s 0.1s;
+
-
    transition: opacity ease-out 0.2s 0.1s;
+
-
}
+
-
 
+
-
a:link{
+
-
  color:#000;
+
-
}
+
-
a:active{
+
-
color:#000;
+
-
}
+
-
a:visited{
+
-
  color:#000;
+
-
}
+
-
a:hover{
+
-
  color:#666;
+
-
}
+
-
 
+
-
#betalactamase{
+
-
top: 400px;
+
-
float:right;
+
-
}
+
-
#antibioticresistance{
+
-
float:left;
+
-
margin-right:0px;
+
-
margin-left:5px;
+
-
}
+
-
#horizontal{
+
-
float:left;
+
-
margin-left:15px;
+
-
}
+
-
 
+
-
#pa{
+
-
float:right;
+
-
text-align: center;
+
-
}
+
-
#bee{
+
-
float: left;
+
-
text-align: center;}
+
-
#quorumsensing{
+
-
float:left;
+
-
text-align:center;
+
-
margin-left:0px;
+
-
}
+
-
 
+
-
.content-1{
+
-
background: #E0ECF8;
+
-
height:1900px;
+
-
}
+
-
.content-2{
+
-
background: #E0ECF8;
+
-
height:1300px;
+
-
}
+
-
 
+
-
.content-3{
+
-
background: #E0ECF8;
+
-
height:1200px;
+
-
}
+
-
.content-4{
+
-
background: #E0ECF8;
+
-
height:1700px;
+
-
}
+
-
 
+
-
#pores{
+
-
float:right;
+
-
margin-top:5px;
+
-
}
+
-
#melittin{
+
-
float:right;
+
-
text-align:center;
+
-
}
+
-
 
+
-
 
+
-
.center{
+
-
margin:auto;
+
-
text-align:center;
+
-
}
+
-
 
+
-
.firstHeading {
+
-
      visibility: hidden;
+
-
}
+
-
#navigation p{
+
-
display:none;
+
-
}
+
-
 
+
-
#navigation a:hover + p{
+
-
display: block;
+
-
}
+
-
 
+
-
</style>
+
-
</head>
+
-
<body bgcolor="#ffffff">
+
-
<div id="yellow_header">  <a href="http://www.stonybrook.edu/">Stony Brook University</a></div>
+
-
 
+
-
<div id="blue_header"> <a href="https://2014.igem.org/Team:Stony_Brook"><img src="https://static.igem.org/mediawiki/2014/1/1e/Stony_Brook_Apis-biotics_header.png"/ id="apis-biotics"/></a>
+
-
 
+
-
<div id="yellow_navigation_container">
+
-
+
-
<div id="yellow_navigation"> <a href="https://2014.igem.org/Team:Stony_Brook"><img src="https://static.igem.org/mediawiki/2014/7/78/Stony_Brook_NavIcon2_Home.png"/></a>
+
-
  <p> Home </p> </div>
+
-
   
+
-
    <div id="yellow_navigation"> <a href="https://2014.igem.org/Team:Stony_Brook/Team"><img src="https://static.igem.org/mediawiki/2014/6/66/Stony_Brook_NavIcon2_Team.png"/></a>
+
-
    <p> Team </p>
+
-
    </div>
+
-
       
+
-
    <div id="yellow_navigation"> <a href="https://2014.igem.org/Team:Stony_Brook/Project"><img src="https://static.igem.org/mediawiki/2014/9/99/Stony_Brook_NavIcon2_Project.png"/></a>
+
-
    <p> Project </p>
+
-
    </div>
+
-
 
+
-
  <div id="yellow_navigation"> <a href="https://2014.igem.org/Team:Stony_Brook/Notebook"><img src="https://static.igem.org/mediawiki/2014/5/57/Stony_Brook_NavIcon2_Notebook.png"/></a>
+
-
  <p> Notebook </p>
+
-
  </div>
+
-
 
+
-
  <div id="yellow_navigation"> <a href="https://2014.igem.org/Team:Stony_Brook"><img src="https://static.igem.org/mediawiki/2014/8/80/Stony_Brook_NavIcon2_Outreach.png"/></a>
+
-
  <p> Outreach </p>
+
-
  </div>
+
-
 
+
-
  <div id="yellow_navigation"> <a href="https://2014.igem.org/Team:Stony_Brook"><img src="https://static.igem.org/mediawiki/2014/0/03/Stony_Brook_NavIcon2_Acknowledgements.png"/></a>
+
-
<p> Attributions </p>
+
-
  </div>
+
-
 
+
-
</div>
+
-
</div>
+
-
 
+
-
<div id="project"><p>Project</p>
+
-
</div>
+
-
 
+
-
<div id="abstract"> <h3>Abstract</h3>
+
-
<p>Pathogenic bacteria are becoming increasingly antibiotic-resistant due to misuse, overuse and abuse. In addition, infections caused by some harmful strains of bacteria, particularly gram-negative bacteria, cannot easily be treated with antibiotics or other common forms of treatment. Our intention is to engineer cells which can both produce an antimicrobial peptide and recognize the communication signals of such bacteria, using Pseudomonas aeruginosa as a model. Two plasmids, one which controls the production of our antimicrobial peptide melittin and the other which acts as the cell-signal receiver, will be inserted in nonpathogenic E. coli cells. This allows our E. coli to recognize the cells of Pseudomonas aeruginosa, and release a bacteria-killing compound in response. </p>
+
-
</div>
+
-
<div id="slideshow"><img src="https://static.igem.org/mediawiki/2014/a/ad/Stony_Brook_Project_Slideshow1.png" height="100%"></div>
+
-
 
+
-
<div id="content">
+
-
<section class="tabs">
+
-
    <input id="tab-1" type="radio" name="radio-set" class="tab-selector-1" checked="checked" />
+
-
    <label for="tab-1" class="tab-label-1">Antibiotic resistance</label>
+
-
   
+
-
    <input id="tab-2" type="radio" name="radio-set" class="tab-selector-2" />
+
-
    <label for="tab-2" class="tab-label-2">P. aeruginosa</label>
+
-
   
+
-
    <input id="tab-3" type="radio" name="radio-set" class="tab-selector-3" />
+
-
    <label for="tab-3" class="tab-label-3">Melittin</label>
+
-
   
+
-
    <input id="tab-4" type="radio" name="radio-set" class="tab-selector-4" />
+
-
    <label for="tab-4" class="tab-label-4">Our Solution: Apis-Biotics</label>
+
-
   
+
-
           
+
-
    <div class="clear-shadow"></div>
+
-
               
+
-
    <div class="content">
+
-
        <div class="content-1">
+
-
            <p>
+
-
            <h4>WHEN ANTIBIOTICS FAIL</h4>
+
-
 
+
-
When Alexander Fleming was accepting his Nobel Prize in medicine for his discovery of penicillin, the world’s first antibiotic, he warned of a future where antibiotics are no longer effective. <sup><a href="#1">[1]</a></sup> The growth of antibacterial resistance—when bacteria no longer are affected by antibiotics—is quickly becoming a major public health concern. In 2014, the World Health Organization stated “A post-antibiotic era—in which common infections and minor injuries can kill—far from being an apocalyptic fantasy, is instead a very real possibility for the 21<sup>st</sup> century.” <sup><a href="#2">[2]</a></sup> </p>
+
-
 
+
-
<h4>THE MECHANISM OF ANTIBIOTIC RESISTANCE</h4>
+
-
 
+
-
<p>Antibiotic resistance is a natural phenomenon that occurs when mutations in bacterial replications render antibiotics ineffective. Many antibiotics kill bacteria by either preventing
+
-
them from creating bacterial cell walls, affecting their DNA, RNA, or proteins, or changing their metabolism. However, through mutations, bacteria may develop the ability to break down <figure id="betalactamase">
+
-
<img src="https://static.igem.org/mediawiki/2014/c/c9/Stony_Brook_Project_Beta_lactam_penicillin_action.png">
+
-
<figcaption>Bacteria are constantly rebuilding their cell walls through peptidoglycan synthesis. Penicillin works by deactivating transpeptidase within the bacteria, preventing the cross-linking of peptidoglycan and weakening the cell wall, eventually causing lysis due to osmotic pressure. Bacteria can develop resistance to penicillin by producing β-lactamase, which breaks the beta-lactam ring in penicillin, deactivating it.</figcaption></figure>these antibiotics, prevent the antibiotic from entering the cell, or use efflux pumps in the cell membrane to transport the antibiotic back outside the cell. When antibiotics are used to kill off harmful bacteria, the resistant strain of bacteria remains and reproduces. With other strains
+
-
gone, this resistant strain has less competition, allowing it to thrive.
+
-
 
+
-
</p>
+
-
 
+
-
<p>However, what occurs more often than mutation-derived resistance is the spread of antibiotic resistance through horizontal gene transfer, which occurs when genetic material from bacteria, such as plasmids, transposons, or DNA are passed from cell to cell. Through this mechanism, bacteria can even develop multi-drug resistance. Even when a singular antibiotic is used for more than ten days, multidrug resistance develops to structurally unrelated drugs as the resistant bacteria recruit resistance genes from other bacteria in the environment, encouraging the development of “super-bugs.” In addition, some bacteria are intrinsically resistant to bacteria, such as gram-negative bacteria, which due to its thicker outer cell membrane are harder to treat with certain classes of antibiotics. In some cases, some strains of gram-negative bacteria are resistant to all antibiotics, even the
+
-
newest families of antibiotics and antibiotics used as a last resort.  <sup><a href="#3">[3]</a></sup>
+
-
<figure id="antibioticresistance"><img src="https://static.igem.org/mediawiki/2014/c/ce/Stony_Brook_Project_Antibiotic_resistance_selection.png"><figcaption>After an antibiotic is used, bacteria with resistance genes are selected while those without resistance die off, allowing resistant bacteria to better compete for resources and thrive</figcaption></figure><figure id="horizontal"><img src="https://static.igem.org/mediawiki/2014/4/43/Stony_Brook_Project_Horizontal_gene_transfer.png"><figcaption> Horizontal gene transfer: DNA for antibiotic resistance is transfered through conjugation.</figcaption></figure>
+
-
</p>
+
-
 
+
-
 
+
-
<p>The misuse of antibiotics in the healthcare environment contributes one avenue for the spread of antibiotic resistance. In one study done for the CDC, researchers found that almost 80% of hospitals misuse antibiotics, either giving inappropriate or redundant antibiotic combinations and intravenous antibiotics. <sup><a href="#4">[4]</a></sup> The use of antibiotics in food and agriculture also presents a significant problem. The Union of Concerned Scientists estimated in one report that 70% of all antibiotics used in the U.S. are used in the food and water of already-healthy livestock, in order to control disease. Many of these antibiotics don’t break down in the waste of livestock, leading to the growth of resistant bacteria which spreads as this waste is used as fertilizer, contaminating the soil and sources of water. This contamination can also be found coming from antibiotic  manufacturing plants, as well as households which improperly dispose of unused or expired
+
-
antibiotic pills, instead or returning them to a pharmacy. <sup><a href="#4">[5]</a></sup></p>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
<br>
+
<br>
<br>
 +
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Stony_Brook/Team&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
 +
</td>
 +
</tr>
-
<h4>TO WHAT EXTENT?</h4>
+
<tr> <td colspan="3"  height="5px"> </td></tr>
 +
<!-- end welcome box -->
 +
<tr>  
-
<p>In its 2014 report on antibiotic resistance, the World Health Organization reported the current status of antibiotic resistance in seven major bacteria which cause disease in humans, including Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Streptococcus pneumoniae. These bacteria are common causes of infections within a hospital or community. If they become highly resistant to antibiotics, there would be significant repercussions for public health
+
<!--navigation menu -->
-
worldwide. </p>
+
<td align="center" colspan="3">
-
<figure class="center"><img src='https://static.igem.org/mediawiki/2014/b/bc/Stony_Brook_Project_Antibiotic_resistance_chart.png'><figcaption> Data taken from WHO 2014 report on antimicrobial resistance </figcaption></figure>
+
<table  width="100%">
 +
<tr heigth="15px"></tr>
 +
<tr heigth="75px">  
-
<h4>WHY DOES IT MATTER?</h4>
 
-
<p>Antibiotic resistance is a global concern—it jeopardizes our ability to treat common infections, causing standard treatments to be replaced by more expensive drug therapies, making infectious diseases longer to treat and easier to spread, putting major surgeries, chemotherapy and organ transplants at a greater risk of being unsuccessful, and increasing death rate. <sup><a href="#2">[2]</a></sup> MRSA, or methicillin-resistant Staphycoccus aureus, and drug-resistant Staphococcus aureus have already
+
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
-
been categorized as “serious threats” by the CDC. <sup><a href="#6">[6]</a></sup>The increasing resistance of these bacteria to the latest generation of antibiotics eventually requires broader spectrum- drugs which have higher costs, higher risks and worse side effects to patients due to a more toxic treatment. The growth of antibiotic resistance in pathogenic bacteria renders one of our most powerful tools against disease useless—making it imperative to develop new courses of treatment.</p>
+
<a href="https://2014.igem.org/Team:Stony_Brook"style="color:#000000">Home </a> </td>
-
<p><h4>REFERENCES</h4>
+
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
-
<ol>
+
<a href="https://2014.igem.org/Team:Stony_Brook/Team"style="color:#000000"> Team </a> </td>
-
<a id="1"></a> <li> Fleming, Alexander. "Nobel Lecture: Penicillin." Speech, Nobel Prize Award Ceremony from Nobel Foundation, Stockholm, December 11, 1945.</li>
+
-
<a id="2"></a><li> World Health Organization. Antimicrobial Resistance: Global Report on Surveillance 2014. S.l.:
+
<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 +
<a href="https://igem.org/Team.cgi?year=2014&team_name=Stony_Brook"style="color:#000000"> Official Team Profile </a></td>
-
World Health Organization, 2014.</li>
+
<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
 +
<a href="https://2014.igem.org/Team:Stony_Brook/Project"style="color:#000000"> Project</a></td>
-
<a id="3"></a><li> Levy, Stuart B, and Bonnie Marshall. "Antibacterial Resistance Worldwide: Causes, Challenges And
+
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 +
<a href="https://2014.igem.org/Team:Stony_Brook/Parts"style="color:#000000"> Parts</a></td>
-
Responses." Nature Medicine 10, no. 12s (2004): S122-S129.</li>
+
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 +
<a href="https://2014.igem.org/Team:Stony_Brook/Modeling"style="color:#000000"> Modeling</a></td>
-
<a id="4"></a><li> Schultz L, Lowe TJ, Srinivasan A, Neilson D, Pugliese G. “Economic Impact of Redundant
+
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
 +
<a href="https://2014.igem.org/Team:Stony_Brook/Notebook"style="color:#000000"> Notebook</a></td>
-
Antimicrobial Therapy in US Hospitals.” Infection Control and Hospital Epidemiliogy 35: 1229-1235.</li>
+
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 +
<a href="https://2014.igem.org/Team:Stony_Brook/Safety"style=" color:#000000"> Safety </a></td>
-
<a id="5"></a><li>. Rosenblatt-Farrell N. “The Landscape of Antibiotic Resistance.” Environ Health Perspect. 117(6):
+
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 +
<a href="https://2014.igem.org/Team:Stony_Brook/Attributions"style="color:#000000"> Attributions </a></td>
-
A244-A250.</li>
 
-
<a id="6"></a><li> Centers for Disease Control and Prevention. Antibiotic resistance threats in the United States, 2013.
+
<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
 +
</tr>
 +
</table>
-
S.l.: Centers for Disease Control and Prevention, 2013.</li></ol></p>
+
<!--end navigation menu -->
-
        </div>
+
</tr>
-
        <div class="content-2">
+
</tr>
-
            <p> <p>
+
</td>
-
<h4>OUR MODEL: PSEUDOMONAS AERUGINOSA</h4>
+
<tr> <td colspan="3"  height="15px"> </td></tr>
 +
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
 +
<tr> <td colspan="3"  height="5px"> </td></tr>
-
<p>Pseudomonas aeruginosa is an example of an opportunistic and virulent pathogen which is strongly resistant against antibiotics. In addition to its developed antibiotic resistance, P. aeruginosa has intrinsic resistance to antibiotics, due to its low membrane permeability as well as pumps in its membrane which actively pump out antibiotics from the cell.<sup><a href="#8">[1]</a></sup> Multi-drug resistant P. aeruginosa is particularly prevalent in hospitals, where it can colonize in hospital equipment and cause cross-infections in patients with weakened immune systems, such as in patients with cystic fibrosis. <sup><a href="#9">[2]</a></sup>  
+
<tr><td > <h3> Team Information</h3></td>
-
 
+
<td ></td >
-
<figure id="pa"> <img src="https://static.igem.org/mediawiki/2014/d/dd/Stony_Brook_Project_Paeruginosa.jpg" height="300" width="300"><figcaption> Biofilm formation of P. aeruginosa (<a href="http://www.uoguelph.ca/~confocal/gallery/Image027Snapshot1.jpg"> Source </a>).</figcaption></figure>
+
<td > <h3> Upload pictures of your team!</h3></td>
-
 
+
</tr>
-
Serious P. aeruginosa infections can cause meningitis, pneumonia, and sepsis. <sup><a href="#10">[3]</a></sup> The CDC estimates that 51,000 healthcare-associated infections in the US are caused by P. aeruginosa, 13% of which are multidrug resistant and which the CDC considers a serious threat. <sup><a href="#11">[4]</a></sup> In addition,  30% of  healthcare-related P. aeruginosa infections are resistant to fluoroquinolones, a broad-spectrum antibiotic. <sup><a href="#12">[5]</a></sup> The resistance of P. aeruginosa can therefore become deadly for immunocompromised patients, leading to higher mortality rates for affected patients.</p>
+
-
 
+
-
 
+
-
 
+
-
<h4>THE QUORUM SENSING SYSTEM OF PSEUDOMONAS AERUGINOSA</h4>
+
 +
<tr>
 +
<td width="45%"  valign="top">
 +
<p>
 +
You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.</p>
<p>
<p>
-
The virulence of P. aeruginosa bacteria is in part controlled by their ability to sense each other by producing and recognizing specific compounds called N-acyl homoserine lactones (AHLs), thus allowing them to communicate with each other.</p> <p>This ability to communicate intercellularly, called quorum-sensing, is used by many bacteria to control population growth through gene activation. <figure id="quorumsensing"> <img src="https://static.igem.org/mediawiki/2014/b/b7/Stony_Brook_Project_Quorum_sensing.png"><figcaption> A simple illustration of quorum sensing: Bacteria produce cell-signaling molecules, and at a certain concentration of cells these molecules interact with other bacteria, serving as a communication system </figcaption></figure>As the bacteria grow, they release signaling signals. Once these signals reach a critical concentration, other bacteria in the environment will recognize these signals and respond in turn by making changes in their gene regulation, allowing them to cooperate with each other as their population reaches a certain density. <sup><a href="#13">[6]</a></sup></p>
+
You can look at what other teams did to get some inspiration! Here are a few examples:</p>
 +
<ul>
 +
<li><a href="https://2013.igem.org/Team:Evry/Team">2013 Evry</a></li>
 +
<li><a href="https://2013.igem.org/Team:Cornell/team">2013 Cornell</a></li>
 +
<li><a href="https://2013.igem.org/Team:UANL_Mty-Mexico/Team">2013 UANL Mty-Mexico</a></li>
 +
<li><a href="https://2013.igem.org/Team:Korea_U_Seoul/Team/Korea_University">2013 Korea U Seoul</a></li>
 +
</ul>
-
<p> P. aeruginosa primarily has two quorum-sensing systems: the las system, which consists of the transcriptional regulator LasR and the synthase protein LasI, and the rhl system, which consists of RhlR and RhlI. LasI and RhlI help in the production of the AHL compounds 3-oxo-C12-HSL and C4-HSL respectively. These compounds bind with their respective transcriptional regulators, LasR or RhlR, which then binds to DNA and regulates the transcription of genes. While both 3-oxo-C12-HSL and C4-HSL freely diffuse out of bacterial cells, 3-oxo-C12-HSL diffuses at a much slower rate than C4-HSL. Both of these systems control the pathogenesis of P.aeruginosa, acting as virulence factors as well as communicative signals and other regulatory factors. <sup><a href="#14">[7]</a></sup></p> <figure class="center"><img src="https://static.igem.org/mediawiki/2014/5/53/Stony_Brook_Project_Rhlr_system.png"><figcaption> As the autoinducer rhli is produced, it forms a complex with C4HSL, which then in turn activates the production of regulatory protein rhlr, which then regulates other genes. </figcaption></figure>
 
-
<p><h4>EXPLOITING THE QUORUM-SENSING SYSTEM</h4>
 
-
By inserting the genes for the rhl sensing system, non-pathogenic i.e. harmless E. coli can be induced to sense P. aeruginosa signals and act in response to them. If a bacteria-killing compound could be produced upon receiving C4HSL or 3-oxo-C6HSL signals, our project could take advantage of naturally-occurring pathogenic bacterial cell communication in order to kill them off. Since C4-HSL diffuses more quickly, we decided to use the rhl system used by P.aeruginosa as a means of identifying our target bacteria. By exploiting the rhl system in P.aeruginosa, we could
+
</td>
-
target them with an antibiotic-like compound, thus killing our bacteria.
+
<td></td>
-
Our next step was to look into our bacteria-killing compound: melittin, a honeybee venom compound.
+
<td width="45%" valign="top">  
-
</p>
+
<p> We provide a server where you can upload images for your wiki!</p>
-
<p><h4>
+
<p> Add pictures of your team members, advisors, instructors, lab, institution and everything you wish to show! </p>
-
REFERENCES</h4>
+
<a href="https://2014.igem.org/Special:Upload"><img src="" width="100px" height="100px"> </a> <a href="https://2014.igem.org/Special:Upload"><img src="" width="100px" height="100px"> </a>
-
<ol>
+
</a> <a href="https://2014.igem.org/Special:Upload"><img src="" width="100px" height="100px"> </a>
-
<a id="8"></a><li> Li XZ, Livermore DM, Nikaido H. 1994. Role of efflux pump(s) in intrinsic resistance of
+
<br>
-
 
+
<a href="https://2014.igem.org/Special:Upload">Click here to upload! </a>
-
Pseudomonas aeruginosa: resistance to tetracycline, chloramphenicol, and norfloxacin.
+
</td>
-
 
+
-
Antimicrobial Agents and Chemotherapy. 38:1732-1741</li>
+
-
 
+
-
<a id="9"></a><li> Döring G, Parameswaran IG, Murphy TF. 2011. Differential adaptation of microbial
+
-
 
+
-
pathogens to airways of patients with cystic fibrosis and chronic obstructive pulmonary
+
-
 
+
-
disease. FEMS Microbiol. Rev. 35:124–146</li>
+
-
 
+
-
<a id="10"></a><li> Bodey GP, Bolivar R, Fainstein V, Jadeja L. 1983. Infections caused by Pseudomonas
+
-
 
+
-
aeruginosa. Rev Infect Dis. 5:279-313.</li>
+
-
 
+
-
<a id="11"></a><li> "Pseudomonas aeruginosa in Healthcare Settings." Centers for Disease Control and
+
-
 
+
-
Prevention. Centers for Disease Control and Prevention, 7 May 2014. Web. 3 June 2014.</li>
+
-
 
+
-
<a id="12"></a><li> Septimus EJ, Kuper KM. 2009. Clinical challenges in addressing resistance to
+
-
 
+
-
antimicrobial drugs in the twenty-first century. Clin Pharmacol Ther. 86(3): 336-339.</li>
+
-
 
+
-
 
+
-
<a id="13"></a><li> Difri, CD. 2008. Difri, CD. 2008. Quorum Sensing: Bacteria Talk Sense. Clin Infect Dis. 47 (8): 1070-1076</li>
+
-
 
+
-
 
+
-
<a id="14"></a><li> Smith RS, Iglewski BH. 2003. Pseudomonas aeruginosa quorum sensing as a potential
+
-
 
+
-
antimicrobial target. J Clin Invest. 112(10): 1460-1465</li></ol></p>  </p>
+
-
        </div>
+
-
        <div class="content-3">
+
-
<h4>HONEYBEE VENOM: PREPROMELITTIN AND MELITTIN</h4>
+
-
<p>Honeybees, or Apis mellifera, are native to Europe, Africa, and western Asia. The introduction of Apis mellifera to other parts of the world began in the 17th century and since then, honeybees can be found around the world. Currently, there are 26 recognized subspecies of Apis mellifera based on morphology and molecular differences, as well as the differences in their habitat.While male bee drones do not have stingers, female bees have a stinger, which acts as a form of defense against predation. It is connected to a sac that releases a venom when the bee stings another organism. <figure id="bee"> <img src="https://static.igem.org/mediawiki/2014/e/e4/Stony_Brook_Project_Apismellifera.jpg" height="300" width="400"> <figcaption> Apis mellifera( <a href="http://en.wikipedia.org/wiki/Italian_bee#mediaviewer/File:Honeybee-27527-1.jpg"> Source </a>)  </figcaption></figure></p>
+
-
<p>Bee venom has been researched for its ability to treat inflammation, infections, and auto-immune diseases. The active portion of the venom causes inflammation and acts as an anticoagulant. The primary active component of bee venom is melittin, which constitutes about 52% of the apitoxin, or bee venom, liquid excreted from the bee. <sup><a href="#15">[1]</a></sup> An antimicrobial peptide, melittin is a part of the host defense system and immune response of honeybees. Previous research has shown it to be effective with some modifications in both treating cancerous cells and HIV.<sup><a href="#16">[2]</a></sup><sup><a href="#17">[3]</a></sup></p>
+
-
 
+
-
<p>Melittin is not produced in bees in its active form. Rather, it is produced in an inactivated form called prepromelittin, which contains the right sequences to send it to the endoplasmic reticulum in bee cells as well as deactivating it.
+
-
Sequence prepromelittin
+
-
Naturally, bees produce prepromelittin in their venom gland, which gets degraded by surrounding enzymes. <sup> <a href="#18">[4]</a></sup> As the prepromelittin is processed in the honeybee cell, the “pre” part of the peptide, which acts as a signal peptide, is cleaved off by signal peptidase enzymes. <sup> <a href="#19">[5]</a></sup> The “pro” sequence, which acts as a protective sequence which inactivates melittin, is slowly cleaved off stepwise by surrounding dipeptidase enzymes as well, allowing the melittin to become liberated.<sup><a href="#20">[6]</a></sup></p>
+
-
<figure class="center"><img src="https://static.igem.org/mediawiki/2014/1/1a/Stony_Brook_Project_Prepromelittin_sequence.png"><figcaption> Sequence of prepromelittin, where green indicates "pre" sequence, red indicated "pro" sequence, and blue indicates the melittin sequence.</figcaption></figure>
 
-
<h4>MELITTIN IN ACTION </h4>
+
</tr>
-
<p>Melittin is a small ampipathic peptide made up of 26 amino acids, with a hydrophobic N-terminus and a hydrophilic C-terminus.As melittin concentration in the membrane accumulates, transient pores are formed which allow the permeability of ions. <figure id="melittin"><img src="https://static.igem.org/mediawiki/2014/0/0c/Stony_Brook_Project_Melittin.jpg"><figcaption>Melittin's alpha-helical structure.</figcaption></figure>As the concentration of melittin increases further, pores are stabilized and thus become large enough to allow the movement of large molecules outside the cell, and at even higher concentrations, the membrane will disintegrate entirely. Effectively, the melittin compound will cause cells to lyse either due to osmotic pressure around the cell, or through a detergent-effect at high enough concentrations.
+
-
Its activity as a cytolytic compound is due to its surface activity. At low concentrations, it adopts an inactive parallel orientation to the cell membrane, and at higher concentrations it adopts a perpendicular orientation to the membrane, which allows it to “wedge” itself in the headgroup space of phospholipids in a phospholipid bilayer, without extending all the way to the center of the bilayer. As a result, the bilayer distorts and curves to fill in the space left by the “wedge”, forming transient pores through the resulting area imbalance between the inner and outer part of the membrane. Through the transient pores, melittin molecules redistribute, eventually forming larger stable pores made up of 4-7 melittin monomers each.<sup><a href="#21">[7]</a></sup> </p>
+
-
<h4>WHY USE MELITTIN?</h4>
+
<tr><td > <h3> Tips for the Team Page </h3></td>
-
<p><figure id="pores"> <img src="https://static.igem.org/mediawiki/2014/7/77/Stony_Brook_Project_Melittin_pore_formation_mechanism.jpg"><figcaption> Mechanism of pore formation (<a href="http://neutron.kaist.ac.kr/research/poreformation.jpg"> Source </a>) </figcaption></figure>Since the majority of antibiotics target parts of bacteria which have mutated to develop resistance, the most effective alternative to antibiotics would work against conserved parts of the bacteria, which cannot mutate easily without compromising its ability to thrive. An example of such an area would be the outermost part of the bacteria, the cell membrane. Melittin can work against pathogenic bacteria by targeting their cell membrane. Thus, melittin can be utilized against pathogenic bacteria as an alternative to antibiotics, as well as a solution to the increasing resistance of bacteria against antibiotics.</p>
+
<td ></td >
 +
<td > </td>
 +
</tr>
-
<h4> REFERENCES</h4>
 
-
<ol><a id="15"></a><li>Meier J, White J. (1995). Clinical toxicology of animal venoms and poisons. CRC Press, Inc. ISBN 0-8493-4489-1.</li>
+
<tr>
-
<a id="16"></a><li>Soman NR, Baldwin SL, Hu G, Marsh JN, Lanza GM, Heuser JE, Arbeit JM, Wickline SA, Schlesinger PH. 2009. Molecularly targeted nanocarriers deliver the cytolytic peptide specifically to tumor cells in mice, reduing cancer growth. J Clin Invest. 199(9): 2830-2842</li>
+
<td width="45%" valign="top">  
-
<a id="17"></a><li>Hood JL, Jallouk AP, Campbell N, Ratner L, Wickline SA. 2013. Cytolytic nanoparticles attenuate HIC-1 infectivity. Antivi Ther. 18(1):95-103</li>
+
<p>What should this page contain?</p>
-
<a id="18"></a><li>Müller G, Zimmermann R. 1987. Import of honeybee prepromelittin into the endoplasmic reticulum: structural basis for independence of SRP and docking protein. EMBO J. 6:2099-2107</li>
+
<ul>
-
<a id="19"></a><li>Mollay C, Vilas U, Kreil G. 1982. Cleavage of honeybee prepromelittin by an endoprotease from rat liver microsomes: identification of intact signal peptide. Proc Natl Acad Sci USA. 79(7): 2260-2263</li>
+
<li> Include pictures of your teammates, don’t forget instructors and advisors! </li>
-
<a id="20"></a><li>Kreil G, Haiml L, Suchanek G. 1980. Stepwise cleavage of the pro part of promelittin by dipeptidylpeptidase IV. Evidence for a new type of precursor-product conversion. Eur J Biochem. 111(1):49-58 </li>
+
<li> You can add a small biography or a few words from each team member, tell us what you like, and what motivates to participate in iGEM. </li>
-
<a id="21"></a><li>Lee MT, Sun TL, Hung WC, Huang HW. 2013. Process of inducing pores in membranes by melittin. Proc Natl Acad Sci USA. 110(35): 14243-8</li></ol>
+
<li>Take team pictures! Show us your school, your lab and little bit of your city.</li>
 +
<li>Remember that image galleries can help you showcase many pictures while saving space </li>
 +
</ul>
-
        </div>
+
</td>
-
        <div class="content-4">
+
-
       
+
-
+
-
+
<td></td>
-
<h4> GST-MELITTIN </h4>  <figure class="center"><img src="https://static.igem.org/mediawiki/2014/7/7f/Stony_Brook_Project_GST-Melittin.png"> </figure><p>In order to prevent melittin from either attacking our own cells or from congregating into inactive tetramers, melittin itself cannot be produced within our E.coli cells. However, if we were to produce the naturally-occurring form of prepromelittin, we would also need to recreate the enzymes within the bee. Reproducing these enzymes in an E. coli cell can quickly become complicated, therefore our project uses the GST tag to inactivate melittin, creating a fusion protein. This tag also allows for easy identification by through gel electrophoresis, allowing us to also see if the melittin was produced. A TEV protease site sits in between the GST tag and melittin, allowing for easy cleavage of the GST tag and activation of melittin upon the addition of the TEV protease.</p>
+
<td width="45%" valign="top"> </td>
-
<h4>RHLR BIOSENSOR</h4>
+
-
<figure class="center"><img src="https://static.igem.org/mediawiki/2014/6/6b/Stony_Brook_Project_RhlR.png"></figure><p>We designed a biosensor which could detect the quorum sensing signals of P. aeruginosa. This biosensor would constitutively produce the RhlR regulatory protein in order to bind with C4HSL molecules, which then would activate the fluorescence marker mCherry. By responding to C4HSL molecules in the environment, our biosensor could then activate the production of our antibacterial compound, melittin. This form of mechanism allows for a more fine-tuned production of melittin, and acts as a preliminary check for biosafety and the efficiency of our circuit.</p>
+
-
<h4>OUR COMPLETE CIRCUIT(RHLR+MELITTIN)</h4>
+
-
<br/>
+
-
<br/>
+
-
<figure class="center"><img src="https://static.igem.org/mediawiki/2014/4/47/Stony_Brook_Project_Entire_circuit.png"></figure>
+
-
<p> In our complete circuit, our <i>E. coli</i> would constitutively produce RhlR proteins, which then could bind with any C4HSL signal molecules in the environment released by P. aeruginosa. Then, this would activate the <i>rhl</i> promoter, starting the production of GST-melittin. We then would exogenously add in TEV protease, in order to cleave off the GST protein and allow the melittin to work against our target bacteria. Both the RhlR and GST-melittin have fluorescence markers, to allow us to easily monitor the progress of the mechanism. In addition, the use of non-specific melittin also acts as a "kill switch", as our <i>E.coli</i> would eventually succumb to the pores created by melittin. </p>
+
-
        </div>
+
</tr>
-
    </div>
 
-
</section>
 
-
</div>
 
-
</body>
 
 +
</table>
</html>
</html>

Revision as of 04:25, 17 October 2014


WELCOME TO iGEM 2014!

Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!


Click here to edit this page!

Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

Team Information

Upload pictures of your team!

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.

You can look at what other teams did to get some inspiration! Here are a few examples:

We provide a server where you can upload images for your wiki!

Add pictures of your team members, advisors, instructors, lab, institution and everything you wish to show!


Click here to upload!

Tips for the Team Page

What should this page contain?

  • Include pictures of your teammates, don’t forget instructors and advisors!
  • You can add a small biography or a few words from each team member, tell us what you like, and what motivates to participate in iGEM.
  • Take team pictures! Show us your school, your lab and little bit of your city.
  • Remember that image galleries can help you showcase many pictures while saving space

Retrieved from "http://2014.igem.org/Team:Stony_Brook/Team"