Team:StanfordBrownSpelman/Material Waterproofing

From 2014.igem.org

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<h5 id="int"><center>First approach: Paper wasp protein</h5>
<h5 id="int"><center>First approach: Paper wasp protein</h5>
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<h6>Paper wasps of the genus <i>Polistes<i> are well known for their ability to construct nests out of transformed plant materials with paper-like properties. The most significant property of the wasp-produced paper is that it is hydrophobic and therefore waterproof. Research has identified that a protein found in the salivary glands of paper wasps is responsible for coating, strengthening, and thus waterproofing the cellulose found in plants. We collected <i>Polistes dominula</i>, an invasive European species of paper wasp, and sequence the proteins found in their saliva using a modified peptide mass fingerprinting approach. Our ultimate goal is to transform the gene coding for the wasp waterproofing protein into <i>Saccharomyces cerevisiae<i> so that we can produce an inherently biomimetic solution to shielding lightweight bacterial cellulose (BC) or BCOAc films from water in the environment. This project is particularly exciting because of its potential for discovery; never before have the proteins in wasp saliva been identified or applied as functional biomaterials.
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<h6>Paper wasps of the genus <i>Polistes</i> are well known for their ability to construct nests out of transformed plant materials with paper-like properties. The most significant property of the wasp-produced paper is that it is hydrophobic and therefore waterproof. Research has identified that a protein found in the salivary glands of paper wasps is responsible for coating, strengthening, and thus waterproofing the cellulose found in plants. We collected <i>Polistes dominula</i>, an invasive European species of paper wasp, and sequence the proteins found in their saliva using a modified peptide mass fingerprinting approach. Our ultimate goal is to transform the gene coding for the wasp waterproofing protein into <i>Saccharomyces cerevisiae<i/> so that we can produce an inherently biomimetic solution to shielding lightweight bacterial cellulose (BC) or BCOAc films from water in the environment. This project is particularly exciting because of its potential for discovery; never before have the proteins in wasp saliva been identified or applied as functional biomaterials.
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<h5><center>Second approach: Wax ester biosynthesis</h5>
<h5><center>Second approach: Wax ester biosynthesis</h5>

Revision as of 07:55, 16 October 2014

Stanford–Brown–Spelman iGEM 2014 — Amberless Hell Cell

Methods
Methods here.


Figure 1. Figure caption here.
Results
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References
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Additional Information
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