Team:StanfordBrownSpelman/Amberless Hell Cell

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Stanford–Brown–Spelman iGEM 2014 — Amberless Hell Cell



Figure 1. Figure caption here.

We developed a novel approach for preventing horizontal transfer of engineered genes into the environment by inserting a UAG-leucine tRNA, and using UAG for leucine in all of the inserted, engineered genes. Because these genes will not be read correctly in other organisms (the UAG will be read as stop, so proteins will be truncated), the engineered genes will not have any effect in naturally-occurring bacteria in the environment. Our project will involve synthesizing UAG-leucine coded versions of the Hell Cell genes and inserting them into the amberless E. coli strain, along with a UAG-leucine tRNA [2]. This will create a strain of bacteria that is both resilient and safe for environmental applications, for example as a biosensor added to the BCOAc membrane using the biotin/streptavidin interaction mentioned above.
Approach & Methods
Methods here.


Figure 2. Figure caption here.
More methods here.


Figure 3. Figure caption here.

Results
Results go here.


Figure #. Figure caption here.
More results here.


Figure #. Figure caption here.
More results here.


Figure #. Figure caption here.
More results here.
References
1. Lajoie MJ et al. (2013) Genomically Recoded Organisms Impart New Biological Functions. Science 342: 357-60. PMID: 24136966.

2. Thorbjarnardóttir, S. et al. (1985) Leucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene. J. Bacteriol. 161: 219–22. PMID: 2981802.
Additional Information
Read about how our submitted Amberless Hell Cell idea was used as a government regulatory case study on synthetic biology. We then began a conversation with Dr. Mark Segal at the EPA about the regulation and safety of the use of engineered bacteria in the environment.

Submitted biobricks: We submitted 9 biobricks for this sub-project. Six of these bricks include parts that can enable other teams to use the Amberless chassis as a system for more responsible synthetic biology.
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