Team:StanfordBrownSpelman/Amberless Hell Cell

From 2014.igem.org

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We developed a novel approach for preventing horizontal transfer of engineered genes into the environment by inserting a UAG-leucine tRNA, and using UAG for leucine in all of the inserted, engineered genes. Because these genes will not be read correctly in other organisms (the UAG will be read as stop, so proteins will be truncated), the engineered genes will not have any effect in naturally-occurring bacteria in the environment. Our project will involve synthesizing UAG-leucine coded versions of the Hell Cell genes and inserting them into the amberless <i>E. coli</i> strain, along with a UAG-leucine tRNA. This will create a strain of bacteria that is both resilient and safe for environmental applications, for example as a biosensor added to the BCOAc membrane using the biotin/streptavidin interaction mentioned above.
We developed a novel approach for preventing horizontal transfer of engineered genes into the environment by inserting a UAG-leucine tRNA, and using UAG for leucine in all of the inserted, engineered genes. Because these genes will not be read correctly in other organisms (the UAG will be read as stop, so proteins will be truncated), the engineered genes will not have any effect in naturally-occurring bacteria in the environment. Our project will involve synthesizing UAG-leucine coded versions of the Hell Cell genes and inserting them into the amberless <i>E. coli</i> strain, along with a UAG-leucine tRNA. This will create a strain of bacteria that is both resilient and safe for environmental applications, for example as a biosensor added to the BCOAc membrane using the biotin/streptavidin interaction mentioned above.
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References: <br>
1. Lajoie MJ <i>et al.</i> (2013) Genomically Recoded Organisms Impart New Biological Functions. <i>Science</i> 342: 357-60. PMID: <a href="http://www.ncbi.nlm.nih.gov/pubmed/24136966" target="_blank">24136966</a>.
1. Lajoie MJ <i>et al.</i> (2013) Genomically Recoded Organisms Impart New Biological Functions. <i>Science</i> 342: 357-60. PMID: <a href="http://www.ncbi.nlm.nih.gov/pubmed/24136966" target="_blank">24136966</a>.
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Revision as of 17:35, 10 October 2014

Stanford–Brown–Spelman iGEM 2014 — Amberless Hell Cell

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Results
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Methods & Safety
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Links & References
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