Team:Reading/Protocols

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A note on protocols		Contents
Below are a number of examples of protocols - modified and non-modified - used throughout our project. The protocols we list below may not have been followed exactly in each instance of usage, often due to time constraints - beginning an experiment on one day and concluding it on another.		A Note on Protocols The Goods Example one Example two Acknowledgements References



The Goods Here are all of the protocols. PCR PCR was used as a means of amplifying each one of our biobrick constructs. Each construct, along with its corresponding flanking sequence of 50-100 bases, was amplified out of each transformed pSB1C3 plasmid. Prior to PCR, it was ensured that all DNA obtained from miniprep was of a concentration of at least 1ng/ul per 100bp; this was performed using a desktop Thermo Scientific NanoDrop machine. All PCR tubes were kept on ice prior to usage. 2μl of each of the VFR (forward) and VR (reverse) primers were added to sterile PCR tubes, along with 2μl of each respective transformed plasmid and 14μl mastermix [add reference here?]. Potassium ferricyanide assay Another potential protocol E. coli transformation Another potential protocol Synechocystis transformation Another potential protocol



References Here are the references.



Acknowledgements Everyone we need to thank for help with protocols.