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Team:Paris Saclay/Notebook/September/8
From 2014.igem.org
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Revision as of 15:41, 9 September 2014
Contents |
Lab Work
D-Lemon Scent
Checking PCR
Electrophoresis of the PCR made Friday
[photo]
PCR with bacteria
From the petri dish (made here, I select some clone to do a PCR : clones 1-2-3 for cad clones 4-5 for PS clones 6 for LS
| Cycle step | Temperature | Time | Cycle |
|---|---|---|---|
|
Bacteria lysis |
95°C |
5 min |
1 |
| Denaturation | 94°C | 30 s | 25 |
| Annealing | 50°C | 25 s | 25 |
| Extension | 72°C | 1 min | 25 |
| Final extension | 72°C | 10 min | 1 |
| Final extension | 8°C | hold | 1 |
