Team:Paris Saclay/Notebook/September/2
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(Created page with "==B-Contruction of the fusion protein== We can see that the blue colonies of E.coli are not blue in a simple petri dish with ampicillin. It's a fail. So, to figure our problem w...")
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(Created page with "==B-Contruction of the fusion protein== We can see that the blue colonies of E.coli are not blue in a simple petri dish with ampicillin. It's a fail. So, to figure our problem w...")
Newer edit →
Revision as of 15:08, 3 September 2014
B-Contruction of the fusion protein
We can see that the blue colonies of E.coli are not blue in a simple petri dish with ampicillin. It's a fail. So, to figure our problem we decided to do a sequencing of our plasmids.
We chose 6 colonies from an Xgal-IPTG petri dish:
- 1 completely blue
- 2 blue with a white ring around the blue
- 3 white colony
PS: we found that there is 2 types of blue colonies: completely blue and blue with a white ring around the blue)
We first did a liquid culture in 5 mL of LB.