Team:Paris Saclay/Notebook/June/30

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Contents

Monday 30th June

Lab work

1 - Culture or the supercompetents:

Mathias

  • MG1655
  • MG1655Z1

1mL of an overnight culture in 100mL of LB.


2 - Réhydration of selected biobricks:

Juliette

  • BBQ_I9026
  • BBQ_K592025
  • BBQ_K786002
  • BBQ_K786003
  • BBQ_K778002
  • BBQ_K592011 {2 times}
  • BBQ_K592009
  • BBQ_K1033910 {idem}
  • BBQ_J45014
  • BBQ_K517003


3 - Transformations :

Maher & Mathias

We use the DH5α provided by our advisors for the aforementioned Biobricks. Spread on petri dishes with the concentration 10^-1 and 10^-2.


4 - Preparations :

  • Aliquots of antibiotics :

Marie & Mathieu

Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. (Do the same for Kanamycin and Ampicillin)

  • Petri dishes

Romain

Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes.


People there:

  • Instructors : Solenne.
  • Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.

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