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From 2014.igem.org

Contents 1 Thursday 17th July 1.1 Lab work 1.1.1 1 - Preparation of supercompetent cells 1.1.2 2 - Bacterial Culture 1.1.3 3 - PCR and Electrophoresis

Thursday 17th July

Lab work

1 - Preparation of supercompetent cells

By Mathias & Romain

Strains used: MG1655 (x2), MG1655Z1 (x2).

Protocol

2 - Bacterial Culture

by Fabio and Maher

Strains used: J23119 from the concentrated colony made the 16th July

5uL of antibiotic in each tube.

3 - PCR and Electrophoresis

by Arnaud & Mathias

PCR targeting with oligos IPS70 and IPS71.

1. Molecular weight marker 1kb
2. Empty
3. IPS70 and IPS71 (1)
4. IPS70 and IPS71 (2)

Protocol

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