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Team:Paris Saclay/Notebook/July/17
From 2014.igem.org
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| - | PCR targeting with oligos IPS70 and IPS71 of | + | PCR targeting with oligos IPS70 and IPS71 of pOSV230 (Apramycine). |
#Molecular weight marker 1kb | #Molecular weight marker 1kb | ||
Revision as of 14:02, 4 August 2014
Contents |
Thursday 17th July
Lab work
1 - Preparation of supercompetent cells
By Mathias & Romain
Strains used: MG1655 (x2), MG1655Z1 (x2).
2 - Bacterial Culture
by Fabio and Maher
2 liquid cultures with 5ml of LB and 5µl of amp (at 9am - 37°C - 150 rpm), both BBa_J23119 from the concentrated colony made the 16th July
3 - PCR and Electrophoresis
by Arnaud & Mathias
PCR targeting with oligos IPS70 and IPS71 of pOSV230 (Apramycine).
- Molecular weight marker 1kb
- Empty
- IPS70 and IPS71 (1)
- IPS70 and IPS71 (2)
Results:
Failure, no PCR products.
Members present:
- Instructors and advisors: Alice and Solenne.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
