Team:Paris Saclay/Notebook/July/17

From 2014.igem.org

(Difference between revisions)
(Thursday 17th July)
(3 - PCR and Electrophoresis)
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
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'''People there:'''
+
'''Members present:'''
* Instructors and advisors: Alice and Solenne.
* Instructors and advisors: Alice and Solenne.
* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]

Revision as of 21:14, 21 July 2014

Contents

Thursday 17th July

Lab work

1 - Preparation of supercompetent cells

By Mathias & Romain

Strains used: MG1655 (x2), MG1655Z1 (x2).

Protocol

2 - Bacterial Culture

by Fabio and Maher

2 liquid cultures with 5ml of LB and 5µl of amp (at 9am - 37°C - 150 rpm), both J23119 from the concentrated colony made the 16th July

3 - PCR and Electrophoresis

Electrophoresis 17.07.2014.jpg

by Arnaud & Mathias

PCR targeting with oligos IPS70 and IPS71 of pOsV230 (Apramycine).

  1. Molecular weight marker 1kb
  2. Empty
  3. IPS70 and IPS71 (1)
  4. IPS70 and IPS71 (2)

Results:

Failure, no PCR products.

Protocol

Members present:

  • Instructors and advisors: Alice and Solenne.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

Back to the calendar