Team:Paris Saclay/Notebook/August/4

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Contents

Monday 4th August

Lab Work

A - The chassis coli Odor free

Striate on Dishes

by Juliette & Romain

We striated MG1655 and MG1655Z1 on LB and Kan dishes, from the striates prepared on LB dishes 1st August

C - Salicylate Inducible Suppressing System

Plasmid DNA Purification

by Eugene and Fabio

  • Ligation of BBa_J61051 and BBa_K228001 made the 30th July

From Liquid Culture made the 1st August

Digestion to check

In progress

Electrophoresis

In progress

D - Lemon scent

Gel electrophoresis of BBa_K762100

by Sean

Samples used were prepared on the 30th July.

Results

From left to right: 5 µl of BBa_K762100 + "old" Phusion enzyme, 5 µl of BBa_K762100 + "new" Phusion enzyme

Electroporation

by Terry

Strain used: DY330 containing plasmid pJBEI6409.

We had to switch the Limonen Synthase cassette with the Apramycin resistance.

The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation.

Polymerase chain reaction

by Sean & Pierre

BBa_K517003

component volume
H2O 35.5μl
Phusion buffer 5X 10μl
dNTPs 2μl
iPS68bis 1μl
iPS69 1μl
DNA 1μl
Phusion enzyme 0.5μl


BBa_K762100

For this BioBrick two tubes were prepared: one with undiluted DNA and one with DNA diluted 10-1.

component volume
H2O 35.5μl
Phusion buffer 5X 10μl
dNTPs 2μl
iPS66 1μl
iPS67 1μl
DNA 1μl
Phusion enzyme 0.5μl

Tubes were placed in PCR machine with the following parameters.

Cycle step Temperature Time Cycle

Initial denaturation

98°C

1 min

1

Denaturation 98°C 15 s 25 - 30
Annealing 52°C 25 s 25 - 30
Extension 72°C 45 s 25-30
Final extension 72°C 10 min 1
Final extension 8°C hold 1

Miscellaneous

By Hoang Vu

Ethics

Well, I just got back from one month of vacation and I'm not really in the mood to do lab works yet. So, I decided to work on ethics first. Synthetic Biology and BioArt are controversial because one is about engineering living organisms, and therefore raises economic, religious, political, moral problems; and the other is about engineering the living organisms too but it's mostly USING the living organisms to do art, to carry a message. This is how I see the essential points of our project ethical problems. But my point of view is not fixed. Maybe I'm wrong, maybe there is something that I have missed. That's why I started with a research on GMOs in general to better understand those issues. - The first trangenic pet were GloFish, "TK-1" 2003 in Taiwan - California is completely against such animals but the funny thing is that California is one of the best supporters of agricultural GMOs. So, there is the question: Why the plants and not the fish? Probably economic benefits and ethical positions are the reasons. Then, is there a hierachy of living organisms? Why? - GMOs under commercial cover raise new problems on environment and commercial rights. - Take some information on the Anti-GMO movement. Members there:

  • Instructors and advisors: Alice, Solenne and Sylvie.
  • Students: Eugene, Fabio, Hoang Vu, Juliette, Pierre, Romain, Sean and Terry.

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