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Team:Paris Saclay/Notebook/August/11
From 2014.igem.org
Contents |
Monday 11th August
Lab work
D - Lemon scent
PCR Clean-up of BBa_K762100
by Sean
PCR prepared on the 7th August.
Clean-up performed with the following protocol.
NB: in the present case we have 90 µl of PCR result and we use 20 µl of elution buffer.
Digestion
by Laetitia and Hoang Vu
-Check the replacement of Limonen synthase gene by Apramycin resistant gene in the pJBEI plasmid.
3 µL plasmid pJBEI 0.5 µ Bgl2
