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Team:Paris Saclay/Notebook/August/11
From 2014.igem.org
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_clean-up Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_clean-up Protocol] | ||
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| + | ===Digestion=== | ||
| + | |||
| + | ''by Laetitia and Hoang Vu'' | ||
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| + | -Check the replacement of Limonen synthase gene by Apramycin resistant gene in the pJBEI plasmid. | ||
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| + | 3 µL plasmid pJBEI | ||
| + | 0.5 µ Bgl2 | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 10:30, 12 August 2014
Contents |
Monday 11th August
Lab work
D - Lemon scent
PCR Clean-up of BBa_K762100
by Sean
PCR prepared on the 7th August.
Clean-up performed with the following protocol.
NB: in the present case we have 90 µl of PCR result and we use 20 µl of elution buffer.
Digestion
by Laetitia and Hoang Vu
-Check the replacement of Limonen synthase gene by Apramycin resistant gene in the pJBEI plasmid.
3 µL plasmid pJBEI 0.5 µ Bgl2
