Team:Paris Bettencourt/Results

Smell The Roses

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Figure 1.Main idea of 'Teen spirit'

Teen Spirit

‘Old people smell’ is a characteristic smell of people over 40 caused by the appearance of 2-nonenal in sweat. In this project we isolated and characterized natural bacteria that can metabolize 2-nonenal and therefore attenuate its smell. These bacteria could be used in a cosmetic product to reduce 2-nonenal in body odor.

Something Fishy

We created a new Biobrick by cloning tmm gene into a Biobrick vector and expressed it in E. coli. The activity of TMM was demonstrated both with a color test and a gas chromatography-mass spectrometer analysis, showing that trimethylamine is degraded and fish odor reduced.

Goody Two Shoes

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Don't Sweat It

- Designed a BioBrick of agaA (main gene responsible for body odor) in pSB1C3.
- Found hotspots for genes ackA and ldh (responsible for body odor) found in human microbiome samples.
- Design CRISPRs to select for natural odorless strains.
- Made a DIY formulation of probiotic cream.

Human practice

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Interlab study

The aim of the Interlab Study was to measure GFP expression under different Anderson promoters and in different plasmids.
iGEM Paris Bettencourt team

• - Successfully transformed E.coli NEB with BBa_I20260 (J23101-B0032-E0040-B0010-B0012) in the pSB3K3 vector - Device 1
• - Successfully built BBa_J23101 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 2
• - Successfully built BBa_J23115 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 3
• - Sequenced and gel verified the three devices
• - Characterized all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader
• - Reported the results on the Interlab Study page of the iGEM wiki
• - Completed the description of the BBa_I20260 - Device 1
• - Submitted and send parts: BBa_K1403000 - Device 2 and BBa_K1403001 - Device 3 to the Biobricks registry

Collaborations

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