Team:Paris Bettencourt/Results

From 2014.igem.org

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<br><br><br>
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<div class=project id=project1>
<div class=project id=project1>
-
<p class=text2></p>
+
 
-
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Odor_Library">Smell The Roses</a></h6><br>
+
<p class=text2></br></br></br></br></p><p class=text1><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Old_People_Smell"><span class=hauteur>Teen Spirit</span></a></p>
-
<p class=text1>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut imperdiet diam eget quam imperdiet imperdiet. Mauris dapibus risus felis, sed ornare diam accumsan aliquet. Sed eu turpis porta, porttitor tortor et, condimentum augue. Curabitur a maximus nisi. Vivamus vitae magna ex. Donec congue auctor odio vitae tempus. In a gravida neque, et tristique tortor. Phasellus a odio sit amet enim ornare lobortis. Morbi sodales, diam non rutrum aliquam, ligula mauris consectetur urna, sed interdum quam risus sit amet enim. Aenean euismod enim magna, id pretium eros molestie non. Proin rutrum lobortis leo, sit amet congue erat. Nulla congue pellentesque augue porta dignissim. Pellentesque quis ex sollicitudin, condimentum risus varius, aliquet ipsum. Ut pulvinar aliquet maximus. Praesent imperdiet interdum commodo. </p>
+
 +
<p class=text1>
 +
-Created BioBricks coding for sequences for different enzymes to nullify the bad odor produced by E. coli.</br>
 +
-We produced the smells that compose the main odor categories perceived by humans.</br>
 +
-BioBricks submitted to be BioBrick registry:BBa_K1403003, BBa_K1403006, BBa_K1403009, BBa_K1403012, BBa_K1403017, BBa_K1403019 </br></p>
 +
 
 +
<p class=text1>
 +
<span class=hauteur><a href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Odor_Library">Smell The Roses</a></span></br>-Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source. -Determined the critical smell detection limit of 2-nonenal.</br>
 +
-Analyzed 2-nonenal tolerance and degradation in natural isolates.</br></p>
</div>
</div>
 +
<p>
<div class=project id=project2>
<div class=project id=project2>
-
<p class=text2></p>
 
-
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Old_People_Smell">Teen Spirit</a></h6><br>
 
-
<p class=text1>‘<ul>
 
-
<li>Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source.</li>
 
-
<li>Determined the critical smell detection limit of 2-nonenal.</li>
 
-
<li>Analyzed 2-nonenal tolerance and degradation in natural isolates.</li>
 
-
</ul></p>
+
<p class=text1></p>
 +
</div>
</div>
<div class=project id=project3>
<div class=project id=project3>
-
<p class=text2></p>
+
</br></br>
-
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/TMAU">Something Fishy</a></h6><br>
+
<p class=text1>
-
<p class=text1><ul id=alignliste>
+
<span class=hauteur><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Foot_Odor">Goody Two Shoes</a></span><br>-Demonstrated that mutant strains of <i>B. subtilis</i> produce less odor.</br>
-
<li>Cloned <i>tmm</i> into <i>E.coli</i> using pSB1C3 and pSEVA315 vectors, creating a new Biobrick: BBa_K1403015.</li>
+
-Measured growth and viability of mutants in synthetic sweat media and on socks.</br> </p>
-
<li>Characterized and quantified the activity of TMM by a colorimetric assay.</li>
+
<p class=text1>
-
<li>Confirmed the degradation of trimethylamine by gas chromatography - mass spectrometry (GC/MS).
+
<span class=hauteur><a href="https://2014.igem.org/Team:Paris_Bettencourt/Project/TMAU">Something Fishy</a></span></br>
-
</ul>. </p>
+
-Cloned <i>tmm</i> into <i>E.coli</i> using pSB1C3 and pSEVA315 vectors, creating a new Biobrick: BBa_K1403015.</br>
 +
-Characterized and quantified the activity of TMM by a colorimetric assay.</br>
 +
-Confirmed the degradation of trimethylamine by gas chromatography - mass spectrometry (GC/MS).</br>
 +
</p>
</div>
</div>
-
<div class=project id=project4>
+
<div class=project id=project4>
-
<p class=text2></p>
+
<p class=text1></p>
-
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Foot_Odor">Goody Two Shoes</a></h6><br>
+
-
<p class=text1>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut imperdiet diam eget quam imperdiet imperdiet. Mauris dapibus risus felis, sed ornare diam accumsan aliquet. Sed eu turpis porta, porttitor tortor et, condimentum augue. Curabitur a maximus nisi. Vivamus vitae magna ex. Donec congue auctor odio vitae tempus. In a gravida neque, et tristique tortor. Phasellus a odio sit amet enim ornare lobortis. Morbi sodales, diam non rutrum aliquam, ligula mauris consectetur urna, sed interdum quam risus sit amet enim. Aenean euismod enim magna, id pretium eros molestie non. Proin rutrum lobortis leo, sit amet congue erat. Nulla congue pellentesque augue porta dignissim. Pellentesque quis ex sollicitudin, condimentum risus varius, aliquet ipsum. Ut pulvinar aliquet maximus. Praesent imperdiet interdum commodo. </p>
+
</div>
</div>
<div class=project id=project5>
<div class=project id=project5>
-
<p class=text2></p>
+
<p class=text1></p>
-
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Eliminate_Smell">Don't Sweat It</a></h6><br>
+
 
<p class=text1>
<p class=text1>
-
- Designed a <b>BioBrick of <i>agaA</i></b> (main gene responsible for body odor) in pSB1C3.</br>
+
<span class=hauteur><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Eliminate_Smell">Don't Sweat It</a></span><br>
-
- Found <b>hotspots</b> for genes <i>ackA</i> and <i>ldh</i> (responsible for body odor) found in human microbiome samples.<br>
+
-Expressed the odor gene <i>agaA</i> in <i>E. coli</i> (BBa_K1403002).</br>
-
- Design <b>CRISPRs</b> to select for <b>natural odorless strains.</b><br>
+
-Designed and used CRISPRs to enrich for loss-of-function mutants 1000x in a mixed population.</br>
-
- Made a DIY formulation of <b>probiotic cream.</b></p>
+
-Identified potential CRISPR targets as odor genes with variability hotspots in metagenome data.</br>
 +
-Formulated a simple skin cream encorporating live bacteria.</br></p>
</div>
</div>
<div class=project id=project6>
<div class=project id=project6>
-
<p class=text2></p>
+
<div class=project id=project7>
-
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Human_Practice">Human practice</a></h6><br>
+
<p class=text1>
-
<p class=text1>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut imperdiet diam eget quam imperdiet imperdiet. Mauris dapibus risus felis, sed ornare diam accumsan aliquet. Sed eu turpis porta, porttitor tortor et, condimentum augue. Curabitur a maximus nisi. Vivamus vitae magna ex. Donec congue auctor odio vitae tempus. In a gravida neque, et tristique tortor. Phasellus a odio sit amet enim ornare lobortis. Morbi sodales, diam non rutrum aliquam, ligula mauris consectetur urna, sed interdum quam risus sit amet enim. Aenean euismod enim magna, id pretium eros molestie non. Proin rutrum lobortis leo, sit amet congue erat. Nulla congue pellentesque augue porta dignissim. Pellentesque quis ex sollicitudin, condimentum risus varius, aliquet ipsum. Ut pulvinar aliquet maximus. Praesent imperdiet interdum commodo. </p>        </div>
+
<span class=hauteur><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study">Interlab study</a></span><br> -Successfully built the three devices</br>
-
<div class=project id=project7>
+
-Sequenced and gel verified the three devices</li>
-
<p class=text2></p>
+
-Characterised all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader</br>-Reported the results on the <a href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study">Interlab Study</a> page of the iGEM wiki</br>
-
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study">Interlab study</a></h6><br>
+
-Completed the description of the <a href="http://parts.igem.org/Part:BBa_I20260">BBa_I20260</a> (Device 1) </br>
-
<p class=text1>The aim of the Interlab Study was to measure GFP expression under different Anderson promoters and in different plasmids.</br>iGEM Paris Bettencourt team
+
-Submitted and send parts: <a href="http://parts.igem.org/Part:BBa_K1403000">BBa_K1403000</a> (Device 2) and <a href="http://parts.igem.org/Part:BBa_K1403001">BBa_K1403001</a> (Device 3) to the Biobricks registry</br> </p>
-
                      <ul class=liste><li>- Successfully transformed <i>E.coli</i> NEB  with <b>BBa_I20260</b> (J23101-B0032-E0040-B0010-B0012) in the pSB3K3 vector - <b>Device 1</b></li>
+
-
                      <li>- Successfully built <b>BBa_J23101 + BBa_E0240</b> (B0032-E0040-B0015) in pSB1C3 backbone - <b>Device 2</b></li>           
+
-
                      <li>- Successfully built <b>BBa_J23115 + BBa_E0240</b> (B0032-E0040-B0015) in pSB1C3 backbone - <b>Device 3</b></li>
+
                     
-
                      <li>- Sequenced and gel verified the three devices</li>
+
                  
-
                      <li>- Characterized all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader</li>
+
-
                      <li>- Reported the results on the <a href'="https://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study">Interlab Study</a> page of the iGEM wiki</li>
+
-
                      <li>- Completed the description of the <a href="http://parts.igem.org/Part:BBa_I20260">BBa_I20260</a> - <b>Device 1</b></li>
+
-
                      <li>- Submitted and send parts: <a href="http://parts.igem.org/Part:BBa_K1403000">BBa_K1403000</a> -<b> Device 2</b> and <a href="http://parts.igem.org/Part:BBa_K1403001">BBa_K1403001</a> - <b>Device 3</b> to the Biobricks registry</li>  
+
-
                      </ul>
+
-
                 </p>
+
</div>
</div>
<div class=project id=project8>
<div class=project id=project8>
-
<p class=text2></p>
+
 
-
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Collaboration">Collaborations</a></h6><br>
+
<p class=text1>
-
<p class=text1>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut imperdiet diam eget quam imperdiet imperdiet. Mauris dapibus risus felis, sed ornare diam accumsan aliquet. Sed eu turpis porta, porttitor tortor et, condimentum augue. Curabitur a maximus nisi. Vivamus vitae magna ex. Donec congue auctor odio vitae tempus. In a gravida neque, et tristique tortor. Phasellus a odio sit amet enim ornare lobortis. Morbi sodales, diam non rutrum aliquam, ligula mauris consectetur urna, sed interdum quam risus sit amet enim. Aenean euismod enim magna, id pretium eros molestie non. Proin rutrum lobortis leo, sit amet congue erat. Nulla congue pellentesque augue porta dignissim. Pellentesque quis ex sollicitudin, condimentum risus varius, aliquet ipsum. Ut pulvinar aliquet maximus. Praesent imperdiet interdum commodo. </p>
+
<span class=hauteur><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Collaboration">Collaborations</a></span><br><b>The Smell Game</b>
-
</div>
+
The web platform continues to evolve, mostly thanks to the people who give their samples and play the game. At this time, users can give a sample of his own sweat, play with the samples of others and then obtain a position of their smell.The SmellGame project relies on a web platform available here :
 +
http://smellofus.synbio4all.org.
 +
Join us and play! </p>
 +
 
 +
<p class=text1>
 +
<span class=hauteur><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Human_Practice">Human practice</a></span><br><b>MOOC</b></br>
 +
-Created a MOOC with 21 lessons to help high school students build a team.</br>
 +
-More than 50 people annotated text in Genius and 15 teachers will use the tool next year.</br>
 +
-20 organizations and iGEM teams around the world have agreed to promote it.</br>
 +
-A student club was created in CRI (Paris) to continue the support for MOOC iGEM HS.</br></br>
 +
<b>Citizen science</b></br>
 +
-More than 600 citizen scientists participated.</br>
 +
-Developing a Smell game for sample analysis</br>
 +
-Living lab event in Cite de Sciences et de l'Industrie.</br>
 +
-Watching a scary movie had little to no affect on body odor intensity.</br>
 +
</br></br>
 +
 
 +
<b>Minder</b></br>Our answer to Tinder is Minder. You can create ideas, rate other users' ideas, view an idea's like/dislike statistics, and find additional details and contact information for ideas you like.
 +
 
 +
 
 +
</p>         </div>
 +
</body>
</body>
</html>
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{{:Team:Paris_Bettencourt/Footer}}
{{:Team:Paris_Bettencourt/Footer}}

Latest revision as of 03:57, 18 October 2014

Smell
the roses
Teen
spirit
Something
fishy
Goody
two shoes
Don't
sweat it





Teen Spirit

-Created BioBricks coding for sequences for different enzymes to nullify the bad odor produced by E. coli.
-We produced the smells that compose the main odor categories perceived by humans.
-BioBricks submitted to be BioBrick registry:BBa_K1403003, BBa_K1403006, BBa_K1403009, BBa_K1403012, BBa_K1403017, BBa_K1403019

Smell The Roses
-Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source. -Determined the critical smell detection limit of 2-nonenal.
-Analyzed 2-nonenal tolerance and degradation in natural isolates.



Goody Two Shoes
-Demonstrated that mutant strains of B. subtilis produce less odor.
-Measured growth and viability of mutants in synthetic sweat media and on socks.

Something Fishy
-Cloned tmm into E.coli using pSB1C3 and pSEVA315 vectors, creating a new Biobrick: BBa_K1403015.
-Characterized and quantified the activity of TMM by a colorimetric assay.
-Confirmed the degradation of trimethylamine by gas chromatography - mass spectrometry (GC/MS).

Don't Sweat It
-Expressed the odor gene agaA in E. coli (BBa_K1403002).
-Designed and used CRISPRs to enrich for loss-of-function mutants 1000x in a mixed population.
-Identified potential CRISPR targets as odor genes with variability hotspots in metagenome data.
-Formulated a simple skin cream encorporating live bacteria.

Interlab study
-Successfully built the three devices
-Sequenced and gel verified the three devices -Characterised all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader
-Reported the results on the Interlab Study page of the iGEM wiki
-Completed the description of the BBa_I20260 (Device 1)
-Submitted and send parts: BBa_K1403000 (Device 2) and BBa_K1403001 (Device 3) to the Biobricks registry

Collaborations
The Smell Game The web platform continues to evolve, mostly thanks to the people who give their samples and play the game. At this time, users can give a sample of his own sweat, play with the samples of others and then obtain a position of their smell.The SmellGame project relies on a web platform available here : http://smellofus.synbio4all.org. Join us and play!

Human practice
MOOC
-Created a MOOC with 21 lessons to help high school students build a team.
-More than 50 people annotated text in Genius and 15 teachers will use the tool next year.
-20 organizations and iGEM teams around the world have agreed to promote it.
-A student club was created in CRI (Paris) to continue the support for MOOC iGEM HS.

Citizen science
-More than 600 citizen scientists participated.
-Developing a Smell game for sample analysis
-Living lab event in Cite de Sciences et de l'Industrie.
-Watching a scary movie had little to no affect on body odor intensity.


Minder
Our answer to Tinder is Minder. You can create ideas, rate other users' ideas, view an idea's like/dislike statistics, and find additional details and contact information for ideas you like.

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
paris-bettencourt-igem@googlegroups.com
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