Team:Paris Bettencourt/Results

From 2014.igem.org

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<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Foot_Odor">Goody Two Shoes</a></h6><br>
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Foot_Odor">Goody Two Shoes</a></h6><br>
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<p class=text1><li>Demonstrated that mutant strains of <i>B. subtilis</i> produce less odor.</li>
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<li>Measured growth and viability of mutants in synthetic sweat media and on socks.</li> </p>
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<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Human_Practice">Human practice</a></h6><br>
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Human_Practice">Human practice</a></h6><br>
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Revision as of 02:28, 18 October 2014

Smell
the roses
Teen
spirit
Something
fishy
Goody
two shoes
Don't
sweat it



Smell The Roses

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut imperdiet diam eget quam imperdiet imperdiet. Mauris dapibus risus felis, sed ornare diam accumsan aliquet. Sed eu turpis porta, porttitor tortor et, condimentum augue. Curabitur a maximus nisi. Vivamus vitae magna ex. Donec congue auctor odio vitae tempus. In a gravida neque, et tristique tortor. Phasellus a odio sit amet enim ornare lobortis. Morbi sodales, diam non rutrum aliquam, ligula mauris consectetur urna, sed interdum quam risus sit amet enim. Aenean euismod enim magna, id pretium eros molestie non. Proin rutrum lobortis leo, sit amet congue erat. Nulla congue pellentesque augue porta dignissim. Pellentesque quis ex sollicitudin, condimentum risus varius, aliquet ipsum. Ut pulvinar aliquet maximus. Praesent imperdiet interdum commodo.

Teen Spirit

  • Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source.
  • Determined the critical smell detection limit of 2-nonenal.
  • Analyzed 2-nonenal tolerance and degradation in natural isolates.

Something Fishy

  • Cloned tmm into E.coli using pSB1C3 and pSEVA315 vectors, creating a new Biobrick: BBa_K1403015.
  • Characterized and quantified the activity of TMM by a colorimetric assay.
  • Confirmed the degradation of trimethylamine by gas chromatography - mass spectrometry (GC/MS).
.

Goody Two Shoes

  • Demonstrated that mutant strains of B. subtilis produce less odor.
  • Measured growth and viability of mutants in synthetic sweat media and on socks.
  • Don't Sweat It

    - Designed a BioBrick of agaA (main gene responsible for body odor) in pSB1C3.
    - Found hotspots for genes ackA and ldh (responsible for body odor) found in human microbiome samples.
    - Design CRISPRs to select for natural odorless strains.
    - Made a DIY formulation of probiotic cream.

    Interlab study

    The aim of the Interlab Study was to measure GFP expression under different Anderson promoters and in different plasmids.
    iGEM Paris Bettencourt team

    • - Successfully transformed E.coli NEB with BBa_I20260 (J23101-B0032-E0040-B0010-B0012) in the pSB3K3 vector - Device 1
    • - Successfully built BBa_J23101 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 2
    • - Successfully built BBa_J23115 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 3
    • - Sequenced and gel verified the three devices
    • - Characterized all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader
    • - Reported the results on the Interlab Study page of the iGEM wiki
    • - Completed the description of the BBa_I20260 - Device 1
    • - Submitted and send parts: BBa_K1403000 - Device 2 and BBa_K1403001 - Device 3 to the Biobricks registry

    Collaborations

    Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut imperdiet diam eget quam imperdiet imperdiet. Mauris dapibus risus felis, sed ornare diam accumsan aliquet. Sed eu turpis porta, porttitor tortor et, condimentum augue. Curabitur a maximus nisi. Vivamus vitae magna ex. Donec congue auctor odio vitae tempus. In a gravida neque, et tristique tortor. Phasellus a odio sit amet enim ornare lobortis. Morbi sodales, diam non rutrum aliquam, ligula mauris consectetur urna, sed interdum quam risus sit amet enim. Aenean euismod enim magna, id pretium eros molestie non. Proin rutrum lobortis leo, sit amet congue erat. Nulla congue pellentesque augue porta dignissim. Pellentesque quis ex sollicitudin, condimentum risus varius, aliquet ipsum. Ut pulvinar aliquet maximus. Praesent imperdiet interdum commodo.

    Centre for Research and Interdisciplinarity (CRI)
    Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
    Paris Descartes University
    24, rue du Faubourg Saint Jacques
    75014 Paris, France
    +33 1 44 41 25 22/25
    paris-bettencourt-igem@googlegroups.com
    Copyright (c) 2014 igem.org. All rights reserved.